Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province

Using double-stranded RNA(dsRNA) technology and sequence-independent amplification(SIA), the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV) Shanxi isolate(ReMV-SX) was sequenced. Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV). The full-length of the obtained ReMV-SX sequence(GenBank accession no. JX575184) was 6 395 nt, containing four open reading frames(ORFs). The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0% homologous to ReMV in Tobamovirus subgroup I, while only 49.8%-58.9% homologous to the isolates in subgroups II and III of the same genus. Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship. The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.     

谈实现城市景观设计完整性的三步骤

结合实例阐述城市景观设计完整性的三大步骤,即环境分析、造景目的和实现技法,并指出景观设计完整性对景观建设的积极意义。     

Rare β-Resorcylic Acid Derivatives from a Halophyte-Associated Fungus Colletotrichum gloeosporioides JS0419 and Their Antifungal Activities

Six new β-resorcylic acid derivatives (1–5 and 7) were isolated from a halophyte-associated fungus, Colletotrichum gloeosporioides JS0419, together with four previously reported β-resorcylic acid lactones (RALs). The relative and absolute stereochemistry of 1 was completely established by a combination of spectroscopic data and chemical reactions. The structures of the isolated compounds were elucidated by analysis of HRMS and NMR data. Notably, compounds 1–3 had a β-resorcylic acid harboring a long unesterified aliphatic side chain, whereas the long aliphatic chains were esterified to form macrolactones in 4–9. Among the isolated compounds, monocillin I and radicicol showed potent antifungal activities against Cryptococcus neoformans, comparable to clinically available antifungal agents and radicicol showed weak antifungal activity against Candida albicans. These findings provide insight into the chemical diversity of fungal RAL-type compounds and their pharmacological potential.     

水分胁迫下不同抗旱类型大豆叶片气孔特性变化研究

采用火棉胶印膜法,研究了不同程度干旱胁迫对不同抗旱类型大豆叶片气孔特性的影响.结果表明:经水分胁迫的大豆叶片气孔密度增加,气孔开口大小和单位叶面积气孔相对面积减小.但不同抗旱类型大豆品种间表现不同,气孔密度变化幅度大小顺序依次为:弱抗品种(A3)＞中抗品种(A2)＞抗旱品种(A1),调节气孔开张能力大小顺序依次为:抗旱品种(A1)＞中抗品种(A2)＞弱抗品种(A3).     

木豆在干热河谷退化山地的生态适应性研究

2000年木豆(Cajanus cajan(L.)Millspaugh)引入云南省元谋干热河谷地区,观测其物候特征、生长适应性、抗旱适应性、抗光热适应性等植物学指标,以了解其对干热退化山地的整体生态适应性.结果表明,木豆能适应干热河谷的退化山地生长环境,其生长势优于原产地,表现出明显的速生性;木豆的抗光热能力较强,能适应于热河谷的不同光照及高温环境,但温度高于37℃,则光合作用停止;木豆的抗旱能力较强,能够通过不同的生理适应性度过旱季,完成其生长周期.木豆适应于干热河谷的生态环境,利用价值较大,是干热河谷退化山地治理与开发的优选灌木.     

几种生长调节剂对四翅滨藜全光喷雾扦插效果的影响

四翅滨藜全光照喷雾嫩枝扦插试验结果表明:采用NAA、ABT 2号生根粉、IBA等3种外源植物生长调节剂处理插条2 h,对插条具有良好的促根作用,其中100 mg/L NAA为最好,生根率可达到93.6%;100 mg/LABT2号生根粉和100 mg/L IBA次之,生根率分别可以达到92.6%,92.3%.     

强化栽培条件下干湿灌溉对水稻生长的影响

在中国水稻研究所试验场研究了强化栽培条件下干湿灌溉对水稻生长的影响.结果表明:在强化栽培条件下采取干湿灌溉处理,在返青期、分蘖期、孕穗期和抽穗开花期比对照分别节水1.4%、12.4%、9.0%和11.2%;水分生产效率达0.93 kg/m3,比CK处理提高34.2%;干4～5 d之后,再复水4～5 d,土壤氧化还原电位循环变化,有利于提高土壤氧化还原电位,改善土壤的通气状况,提高根系活力,促进大穗,穗粒致明显增加,单产平均提高6.9%.     

基于生态系统视角的乡村旅游空间地域性探讨

尽管人们对乡村旅游作了大量的研究,但对基本概念仍未取得一致。文章在对国内外研究情况综述的基础上,从生态系统的新视角对乡村旅游空间地域性进行了探讨,认为这对相关概念的辨析、指导乡村旅游发展规划等有积极的意义。     

黄河干流梯级水库补偿调节方案风险分析

本文以黄河干流梯级水库补偿调节为例,系统分析了补偿调节方案的主要风险因素,提出了多因素组合风险估计的蒙特卡罗(Monte-Carlo)随机模拟技术,并根据风险指标对补偿调节方案的风险做出评价。结果表明,研究建立的补偿调节方案风险模型是合理可行的。     

乌鲁木齐都市圈投资环境分析

随着我国同中亚五国经济合作的不断升温,加上乌鲁木齐都市圈优越的自然条件和独特的区位优势,其面临着经济发展前所未有的机遇。因此,如何尽快改善该区的投资环境,吸引更多资金,是一个亟待解决的课题。本文从地理、经济、政治、社会等方面全面分析了整个都市圈的投资环境,在分析其优势和劣势的同时,提出了可行性的建议和对策,目的在于发挥优势,减小劣势,提高综合投资环境水平,促进经济的快速发展。