Influence of transection of the cervical sympathetic trunk on content of NO and expression of NOS in placenta of rats with pregnancy-induced hypertension syndrome

AIM:To observe the influence of transection of the cervical sympathetic track (TCST) on the content of NO and the expression of eNOS mRNA and iNOS mRNA in placenta of the rats with pregnancy-induced hypertension syndrome (PIH).METHODS: Pregnant Wistar rats were randomly divided into 5 groups: control group (C): saline was injected subcutaneously from 14th day to 20th day of gestation;PIH group 1 (H1) and group 2 (H2): L-NAME was respectively injected with 125 mg/kg and 62.5 mg/kg,respectively,then the other procedures were the same as group C;Operation group (O): TCST was operated on 14th day of the gestation,then the other procedures were the same as group H1;sham operation group (S): the cervical sympathetic trunk was only separated and exposed on 14th day of the gestation,then the other procedures were the same as group H1.RESULTS: (1) Except the base value of the BP and protein in urine of the pregnant rats,all the parameters observed in group H1 and H2 were higher than those in group C significantly (P<0.01),and in group H2 were lower than those in group B1 markedly (P<0.01).(2) In comparison with those in group C,the size and body weigh of fetus in group H1,H2 decreased markedly (P<0.01).The above indexes in group H1 were lower than those in group H2 markedly (P<0.01,P<0.05).The changes of the rate of embryo absorption and fetal death,and deformity rate of the fetal rats were contrary to the above indexes.(3) The content of NO and the expression of eNOS mRNA and iNOS mRNA in placenta in group H1 and H2 were lower than those in group C markedly (P<0.01).Those in group H1 were lower than those in group H2 obviously (P<0.01,P<0.05).Those in group O were higher than those in group H1 markedly (P<0.01).CONCLUSION: TCST protects pregnant rats against PIH,and it was related to the mRNA expression of eNOS and iNOS and the content of NO in placenta tissue.     

Exercising the Pregnant Mare from Day 16 to Day 80 of Gestation

Our previous research has demonstrated moderate exercise can be detrimental to early pregnancy in the mare, but little work has examined exercise after pregnancy has been detected. We exercised mares (n = 8) 6 days a week for 45 min from Day 16 until Day 80 of gestation. Color Doppler ultrasonography was used to evaluate embryonic vesicle size, fetal length, and uterine blood flow. Blood was sampled every other day to analyze cortisol and progesterone concentrations. Results indicated that exercising pregnant mares (n = 4) led to greater (P < .01) cortisol concentrations 30 min after the exercise period. No overall treatment effect could be detected in progesterone concentrations; however, following Day 60 of gestation, progesterone concentrations were lower (P < .05) in exercised mares. Additionally, progesterone concentrations peaked earlier in exercised mares at Day 52 of gestation compared to peak levels at Day 68 of gestation for control mares. No significant effects were detected in embryonic vesicle size. Fetal length tended (P = .06) to be longer in the conceptus of exercised mares. Uterine blood flow did not differ between groups but increased as pregnancy progressed (P < .001) in both groups. All mares in this study went on to deliver healthy foals and suffered no difficulties during parturition. These results indicated that moderate exercise was not detrimental to mare pregnancy.     

Relationship of Progesterone,Bovine Pregnancy-Associated Glycoprotein-1 and Nitric Oxide with Late Embryonic and Early Fetal Mortalities in Dairy Cows

The aim of the present study was to determine the relationship of progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 to day 42) and early fetal mortalities (EFM; > day 42 to day 56) in dairy cows. Transrectal ultrasonography (6–8 MHz) was performed in 100 Holstein-Friesian cows at days 28, 42 and 56 after artificial insemination (AI; day 0) to diagnose pregnancy and to monitor the fate of the embryo. After ultrasound scanning of each cow, a milk sample was collected for assessment of P4 by an ELISA test and a blood sample was collected for assessment of bPAG-1, by using a double-antibody radioimmunoassay, and serum NO metabolites (nitrate + nitrite). Based on ultrasonographic examinations and bPAG-1-RIA, 41 of 100 inseminated cows were confirmed pregnant at day 28 after AI. Nine cows suffered of LEM, and 6 cows suffered of EFM and the overall pregnancy loss rate was 36.6% (15/41) between days 28 and 56 of pregnancy. By logistic regression analysis, there were no significant relationships between the level of P4 and bPAG-1 at day 28 after AI and the occurrence of LEM and EFM. Also, there were no significant relationships between the levels of P4 and bPAG-1 at day 42 and the occurrence of EFM. On the other hand, a significant relationship (P<0.05) was found between NO level at day 28 and the occurrence of LEM. In conclusion, measurement of the serum NO concentration at day 28 of pregnancy might help to predict the outcome of pregnancy by day 42 in dairy cows but further studies are needed to confirm this.     

蒙古族草原游牧文化(Ⅵ)——蒙古族的游牧生活与文体文化

通过查阅资料和调查总结了蒙古族的游牧生活与文体文化。蒙古族牧民乌琦大皮极为适应高原日温差大的自然环境,耐贮藏富营养的肉、奶、面食品和蒙古包最适合游牧生活方式。总之,蒙古族牧民的衣、食、住、行都与环境相适应。蒙古族牧民能歌善舞,创造了称为"草原文化三宝"的长调、马头琴、呼麦。其中,蒙古族长调民歌被联合国教科文组织列为"人类口头和非物质遗产代表作"。中国第1个文化遗产日颁布的第一批非物质文化遗产名录中,就有蒙古族长调民歌、呼麦、格斯尔、乌力格尔、安代舞、达斡尔族鲁日格勒舞、蒙古族马头琴音乐、成吉思汗祭典、鄂尔多斯婚礼、祭敖包、那达慕等17项。蒙古族的竞技体育活动包括骑马、摔跤、射箭、赛跑、游泳、抛套索"等,这些活动也与狩猎、游牧活动密切相关。蒙古族牧民创造的鹿棋、蒙古象棋、嘎拉哈、古尔等游戏丰富了中华牧业文化遗产宝库。     

鲈鱼与养生

在广泛文献检索基础上,对鲈鱼的种属、营养成分、养生价值、代表性膳食及食用注意事项进行了概述,以期为全民养生保健提供科学资料。     

Changes in fetal mannose and other carbohydrates induced by a maternal insulin infusion in pregnant sheep

Background

The importance of non-glucose carbohydrates, especially mannose and inositol, for normal development is increasingly recognized. Whether pregnancies complicated by abnormal glucose transfer to the fetus also affect the regulation of non-glucose carbohydrates is unknown. In pregnant sheep, maternal insulin infusions were used to reduce glucose supply to the fetus for both short (2-wk) and long (8-wk) durations to test the hypothesis that a maternal insulin infusion would suppress fetal mannose and inositol concentrations. We also used direct fetal insulin infusions (1-wk hyperinsulinemic-isoglycemic clamp) to determine the relative importance of fetal glucose and insulin for regulating non-glucose carbohydrates.

Results

A maternal insulin infusion resulted in lower maternal (50%, P < 0.01) and fetal (35-45%, P < 0.01) mannose concentrations, which were highly correlated (r² = 0.69, P < 0.01). A fetal insulin infusion resulted in a 50% reduction of fetal mannose (P < 0.05). Neither maternal nor fetal plasma inositol changed with exogenous insulin infusions. Additionally, maternal insulin infusion resulted in lower fetal sorbitol and fructose (P < 0.01).

Conclusions

Chronically decreased glucose supply to the fetus as well as fetal hyperinsulinemia both reduce fetal non-glucose carbohydrates. Given the role of these carbohydrates in protein glycosylation and lipid production, more research on their metabolism in pregnancies complicated by abnormal glucose metabolism is clearly warranted.     

繁殖障碍母牛催奶试验

通过对21头产后9个月未孕母牛进行催奶处理,结果表明:母牛平均产奶量由处理前的(9.66±2.58)kg/d增加到处理后的(12.0±3.7)kg/d,平均每头增加2.43 kg/d,检查(P<0.05)差异显著;处理后奶牛的受胎率为71％。     

干扰素-τ(IFN-τ)的分子结构和妊娠识别机理

在反刍动物的整个妊娠失败中，早期胚胎死亡占有很大的比例，它在很大程度上是由母体妊娠识别失败造成的IFN-τ（干扰素-τ）是由反刍动物孕体附植时发出的特有的妊娠识别信号，它是建立母体妊娠识别的一种主要因子，本文阐述了IFN-τ的分子结构及其最新研究进展，即IFN-τ和PGF2α，UCRP，OTR，GM-CSF的互作机理。     

利用硫酸铜液诊断母牛早期妊娠技术初探

采用3%和5%硫酸铜液对57头黑白花母牛进行早期妊娠诊断,然后通过直肠检查技术进行确诊。结果表明,3%硫酸铜液乳检的总阳性率为87.14%,5%硫酸铜液乳检的总阳性率为84.80%,两组均以配种后31~50d中午乳样检出准确率最高,平均阳性率为90.35%。5%硫酸铜液乳检的阳性符合率较3%硫酸铜液稍低,即造成误诊空怀率稍高,实际应用中以3%硫酸铜液进行母牛早期妊娠诊断,其准确率较高。     

不同采食量对妊娠期和泌乳期新西兰母兔水平衡影响的研究

本研究为对母兔妊娠期和哺乳期两个生理阶段水平衡的测定。选用的16只经产妊娠期新西兰母兔,随机分成四组,每组4只。试兔采食同一营养密度日粮。对照组为充分饲养组,日采食量为260克;其余三组的采食量各为对照组的90、80和70％(即各为230克、210克和190克)。结果表明:处于同一生理阶段试兔的水平衡不受饲料采食量多少的影响。母兔妊娠期每公斤体重每天的水平衡为18.9克;泌乳期为42.0克,差异极显著(P<0.01)。可见兔的生理状态是影响兔水平衡的重要因素。测得母兔妊娠期饮水占总摄水量的68.9％,泌乳期饮水占总摄水量的84.7％。母兔妊娠期每公斤体重饮水需112.7克,泌乳期需167.0克,饮水与饲料水之比各为1.49∶1和2.11∶1。由此表明:加强繁殖母兔的饲养管理不应忽视对其供给充足、洁净的饮水。