菜用大豆和普通大豆叶片内源激素活性对钾素营养响应的差异研究

以菜用大豆品系121、中科毛豆1号和普通大豆东生7号为材料,采用酶联免疫吸附法,在正常施用氮肥和磷肥的基础上,施用不同的钾肥(K0:不施钾肥;K1:K_2SO_4120 kg·hm~(-2);K2:K_2SO_4120 kg·hm~(-2)并在花期和荚期喷施1%K_2SO_4叶面肥),研究了菜用大豆与普通大豆籽粒形成期叶片中内源激素的活性变化,结果表明:开花后28~56 d菜用大豆和普通大豆叶片中内源激素IAA、GA、ZR和ABA的变化动态差异明显,其中GA和ZR的变化动态趋势相反。施用钾肥提高菜用大豆叶片中IAA、GA和ZR的含量(K2K1K0),降低ABA的含量(K0K1K2),普通大豆叶片中IAA、GA和ZR的含量对钾肥施用的响应与菜用大豆不同(K1K2K0),但是钾肥施用对菜用大豆和普通大豆叶片中ABA含量的影响一致(K0K1K2)。施钾提高了菜用大豆和普通大豆籽粒形成期叶片中IAA/ABA,GA/ABA和ZR/ABA的比值,可能是提高大豆产量和改善品质的部分生理机制。     

青海河卡牧场硒的季节变化及盈缺分析

试验研究了青海共和盆地河卡牧场夏、冬两季牧草草地系统(水、土壤、牧草、绵羊)中硒的含量变化.结果表明,河卡牧场夏季土壤和牧草严重缺硒,绵羊血浆中硒浓度低于临界水平,夏冬两季绵羊被毛中硒含量低于毛硒含量临界值.     

洋山海域大型底栖动物年际生态变化的初步研究

依据2003～2005年洋山海域大型底栖动物的监测结果,运用y、C、d、H’和J等参数,结合聚类分析探讨了该海域大型底栖动物的种类数、生物量、栖息密度和多样性指数的年间变化情况。结果表明,2003～2005年底栖动物出现种类数依次为2003年（21种）〉2004年（17种）〉2005年（16种）,种类数趋于减少;生物量呈现2003年（4．26g／m2）〉2005年（2．18g／m2）〉2004年（0．62g／m2）,栖息密度2003年（31．10ind／m2）〉2004年（12．50ind／m2）〉2005年（11．00ind／m2）,Shannon-Wiener多样性指数（H）、均匀度指数（J）、丰富度指数（d）逐渐降低,单纯度指数（C）相应上升。通过对各次监测结果的聚类分析,将聚类结果分为两类：2003年2、5和8月为一类,2004～2005年6次监测为一类,2003年与2004～2005年的大型底栖动物生态变化明显,洋山深水港工程建设对洋山海域大型底栖动物造成了影响。     

建党以来农业税制的历史变迁

中国共产党自诞生起就是农民利益的忠实代表,在制定经济政策的过程中,总是尽可能地把农民阶级的利益最大化,这从中国共产党根据不同的历史条件下,制定、调整、取消农业税制的过程中可窥一般。本文仅对中国共产党在局部执政时及全面执政后所制定的农业税制的变革及实施情况作以梳理,并期对资政存史有所裨益。     

葡萄叶片光合速率日间降低内外因调控的研究

土壤水分充足时,葡萄日间光合速率的下降主要归因于CO2叶肉阻力的升高;光照、叶温和大气湿度可以影响气孔对CO2的阻力,但主要通过影响叶肉阻力来影响光合速率。‘巨峰’和‘长相思’葡萄具有不同的光合作用光响应曲线及其特征参数。     

实验性鸡马立克氏病羽髓病变研究

通过光镜1电镜等方法对鸡马立克氏病羽髓组织进行了形态学研究。结果显示，羽髓远端坏死，羽髓中有多量肿瘤细胞浸润，肿瘤细胞呈弥漫性或局部性浸润，电镜观察，肿瘤细胞核异型性明显，核膜凹陷，核内出现假核内包含物，在一些羽髓中，巨噬细胞吞噬肿瘤细胞较多见，而且观察到一些肿瘤细胞发生调亡，结果表明，鸡马立克氏病羽髓病变十分明显，羽髓的形态学变化可用于鸡马立克氏病的诊断。     

Effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia

AIM: To investigate the effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells (PASMCs) from rats exposed to chronic hypoxia. METHODS: (1) Wistar rats were randomly divided into control group (group A) and chronic hypoxia group (group B). Then group B received hypoxia 8 hours per day for 4 consecutive weeks. (2) Single PASMC was obtained via acute enzyme separation method. (3) Conventional whole-cell patch clamp technique was used to record resting membrane potential (Em) and ion currents of voltage-gated potassium channel. The changes of ion currents of voltage-gated potassium channel before and after applying cGMP (1 mmol/L), an agonist of protein kinase G (PKG), and cGMP plus H-8 (1 mmol/L), an inhibitor of PKG were compared between two groups. RESULTS: The Em of group B were significantly lower than that of group A. The ion currents of voltage-gated potassium channel in group A and group B were all significantly inhibited by cGMP [control group: from (118.0±5.0) pA/pF to (89.9±16.5) pA/pF, n=6, P＜0.05;chronic hypoxia group: from (81.0±5.0) pA/pF to (56.8±9.1) pA/pF, n=6, P＜0.05]and these effects were reversed by H-8 [control group: from (119.2±10.3) pA/pF to (117.8±9.1) pA/pF, n=6, P＞0.05;chronic hypoxia group: from (96.8±6.2)　pA/pF to (98.0±2.2)　pA/pF, n=6, P＞0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.     

基于MCI的新疆近60 a干旱时空特征分析

利用新疆99个气象站点1961-2020年逐日气象数据,根据最新修订的《气象干旱等级》国标,计算各站点1961-2020年逐日气象干旱综合指数序列(MCI).利用相关分析、线性趋势、突变检验、EOF等方法,分析了近60a新疆干旱强度的时空变化特征.结果 表明:(1)新疆MCI监测结果与降水距平百分率、干旱灾情记录具有较...     

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.