2910年国内外蜂胶研宄概况（一）

对2010年国内外蜂胶的研究概况进行了综述,对公开发表的227篇中英文文献及11篇硕士学位论文的研究领域、地域分布、刊物分布等情况进行了统计分析和评价,并重点介绍了蜂胶化学成分、药理活性、提取工艺、加工工艺、质量控制以及在农业、畜牧业等方面的研究与应用新进展。     

Case–control risk factor study of methicillin-resistant Staphylococcus pseudintermedius (MRSP) infection in dogs and cats in Germany

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a highly drug-resistant small animal veterinary pathogen. Although often isolated from outpatients in veterinary clinics, there is concern that MRSP follows a veterinary-hospital-associated epidemiology. This study's objective was to identify risk factors for MRSP infections in dogs and cats in Germany. Clinical isolates of MRSP cases (n = 150) and methicillin-susceptible S. pseudintermedius (MSSP) controls (n = 133) and their corresponding host signalment and medical data covering the six months prior to staphylococcal isolation were analysed by multivariable logistic regression. The identity of all MRSP isolates was confirmed through demonstration of S. intermedius-group specific nuc and mecA. In the final model, cats (compared to dogs, OR 18.5, 95% CI 1.8–188.0, P = 0.01), animals that had been hospitalised (OR 104.4, 95% CI 21.3–511.6, P < 0.001), or visited veterinary clinics more frequently (>10 visits OR 7.3, 95% CI 1.0–52.6, P = 0.049) and those that had received topical ear medication (OR 5.1, 95% CI 1.8–14.9, P = 0.003) or glucocorticoids (OR 22.5, 95% CI 7.0–72.6, P < 0.001) were at higher risk of MRSP infection, whereas S. pseudintermedius isolates from ears were more likely to belong to the MSSP-group (OR 0.09, 95% CI 0.03–0.34, P < 0.001). These results indicate an association of MRSP infection with veterinary clinic/hospital settings and possibly with chronic skin disease. There was an unexpected lack of association between MRSP and antimicrobial therapy; this requires further investigation but may indicate that MRSP is well adapted to canine skin with little need for selective pressure.     

浅谈高尔夫球场建造的成本控制

高尔夫球场建造项目经济利润是高尔夫施工单位存在和长期发展的基础,也是施工单位追求的最终目标.高尔夫施工单位项目利润主要来源于建造工程成本的控制.随着市场经济的深入发展,高尔夫项目建造竞争越来越激烈,施工单位的利润空间越来越低.通过有效措施来控制高尔夫球场建造的成本,成为施工单位获得最大盈利的重点.本研究通过阐述高尔夫球...     

家畜性别控制技术的研究进展

在畜牧生产中,人为控制出生家畜的性别可以降低生产成本,提高经济效益。随着科技的进步和研究的不断深入,家畜性别控制技术也在不断的完善。作者综述了X、Y精子的分离和胚胎性别鉴定这两项家畜性控技术的发展历程和研究现状,并对其今后的研究方向及应用前景进行了展望。     

副猪嗜血杆菌病研究进展

副猪嗜血杆菌(HPS)是猪上呼吸道的一种常见病原菌.在一定的条件下,可引起猪的格拉瑟病(Glasser's disease),常与猪流感病毒并发,引起断奶前后仔猪以多发性浆膜炎、关节炎、脑膜炎等为特征的临床症状,给养猪业带来很大的损失.为了更加全面的了解该病原菌,论文就近年来副猪嗜血杆菌病的病原学、流行病学、致病机理、...     

An Outbreak of Respiratory Disease Complex in Sheep in Central Ethiopia

An investigation was carried out into an outbreak of respiratory disease complex (RDC) in 3641 Menz and Awassi×Menz cross sheep in Central Ethiopia between 1998 and 1999 by clinical, serological, microbiological, post-mortem and histopathological examinations. The monthly incidence of RDC varied from 2.8% to 4.0% and the prevalence was as high as 17%. The case fatality rate was 18%, despite culling of sick sheep. Over 76% of the morbidity occurred in adults, followed by 19% among weaners. Similarly, 62% of the mortality was in adults. However, 27% of the mortality occurred in lambs despite the low morbidity in the group. Significant breed and age differences were seen in the morbidity and mortality rates (p<0.05). Clinical signs, gross and microscopic lesions and serological and bacteriological examinations showed an interplay of several causes of the RDC, including pestes des petits ruminants (PPR) (72.3%, serologically confirmed), lung worms, maedi-visna, bacterial bronchopneumonia (staphylococcal and streptococcal), enzootic pneumonia and some fungal infections. Cold temperatures, which may be as low as –8.5°C at night, are major predisposing factors along with managemental stresses. Vaccination of animals with a homologous PPR vaccine appeared to decrease dramatically the occurrence of the disease, showing that PPR played an important role in the outbreak. Several of the pathogens do not appear to be individually capable of causing the respiratory disease. Appropriate strategies for the prevention of RDC are suggested.     

Epidemiology and control of Menangle virus in pigs

OBJECTIVE: To describe the epidemiology and eradication of Menangle virus infection in pigs. DESIGN: Field observations and interventions, structured and unstructured serological surveys, prospective and cross-sectional serological studies and laboratory investigations. PROCEDURE: Serum samples were collected from pigs at a 2600-sow intensive piggery in New South Wales that experienced an outbreak of reproductive disease in 1997. Serum samples were also collected from piggeries that received pigs from or supplied pigs to the affected piggery and from other piggeries in Australia. Serum and tissue samples were collected from pigs at piggeries experiencing reproductive disease in New South Wales. Sera and faeces were collected from grey-headed flying foxes (Pteropus poliocephalus) in the region of the affected piggery. Serum samples were tested for neutralising antibodies against Menangle virus. Virus isolation was attempted from faeces. RESULTS: Following the outbreak of reproductive disease, sera from 96% of adult pigs at the affected piggery, including sows that produced affected litters, contained neutralising antibodies against Menangle virus. Neutralising antibodies were also detected in sera from 88% of finisher pigs at two piggeries receiving weaned pigs from the affected piggery. No evidence of Menangle virus infection was found in other piggeries in Australia. In cross-sectional studies at the affected piggery, colostral antibodies were undetectable in most pigs by 14 to 15 weeks of age. By slaughter age or entry to the breeding herd, 95% of pigs developed high antibody titres (> or = 128) against Menangle virus in the virus neutralisation test. Menangle virus was eradicated from the affected piggery following a program of serological testing and segregation. Neutralising antibodies against Menangle virus were also detected in P poliocephalus from two colonies in the vicinity of the affected piggery. Two piggery workers were infected with Menangle virus. There was no evidence of infection in cattle, sheep, birds, rodents, feral cats and a dog at the affected piggery. CONCLUSIONS: Serological evidence of infection with Menangle virus was detected in pigs at a piggery that had experienced reproductive disease, in pigs at two associated piggeries and in fruit bats in the region of the piggery. Two humans were infected. The mode of transmission between pigs is unknown, but spread by faecal or urinary excretion is postulated. This virus can be eradicated by the segregation of pigs into discrete age groups.     

种畜禽场疫病防控与净化现状分析

伴随着我国畜牧业的快速发展,疫病防控难度日益增加,成为制约畜牧业健康发展的重要因素。改变传统疫病防控模式、实施疫病净化是综合提升养殖场生物安全水平和动物抗病能力的有效手段,是推动养殖业持续向好发展的重要基础。本文就种畜禽场疫病净化现状进行分析并提出建议。