荧光成像技术在植物病害检测的应用研究进展

植物病害阻碍植物正常生长,对蔬菜产品品质安全造成威胁,病害检测可以有效控制植物病害发展,是提高果蔬品质安全和可持续生产能力的一个重要途径。所有的绿色植物在受到紫外光或可见光激发时,都会发出可见光波段的荧光。基于植物荧光的成像技术,是利用计算机模拟人眼的视觉功能,在不破坏植株外观且不影响其生长的情况下,获取植物的荧光图像,并通过图像处理算法,对植物的健康状况做出判断。本文对荧光成像技术及其在植物病害检测中的研究进行了综述,介绍了荧光成像的原理,分别从叶绿素荧光和其他色素荧光两方面总结了国内外研究现状。与可见光成像、高光谱成像、多光谱成像以及热成像技术相比,该技术具有可获取植物内部信息、受温湿度影响小、不局限于晴天采集、价格合理等优点。     

基于无人机影像匹配点云的苗圃单木冠层三维分割

近年来较多的树冠提取算法以激光雷达数据为基础,然而激光点云数据量大、冗余多而且采集成本高。本文基于无人机影像匹配点云提取单木树冠轮廓,研究一种成本可控、能够补充甚至部分替代激光雷达的小范围森林制图方案。以福建省三明市某林场内苗圃地作为研究对象,在稠密的无人机影像匹配点云中截取2个25 m×25 m的样地作为测试样本。预处理后,首先构建植被冠层高度模型,以局部最大值法探测树冠位置并标记为种子点;从这些种子点形成的初始区域开始生长,迭代计算直到全部的影像匹配点云归并完毕;最后,将算法提取的树冠轮廓导入Arc GIS中获取树冠轮廓矢量边界,并与手绘参考树冠叠加,利用F测度实现精度的评定。依此方案,在2个林分范围内的树冠提取F测度均达到了89%以上,单木冠幅提取的误差在0.14 m以内。结果表明,该方案简单有效、精度可靠,适用于小范围、高精度的植被制图。     

骨干淤地坝等高度及无约束分期加高的研究

以总工程费现值最小为目标函数,对等高度和无约束分期加高的优化计算方法和分析结果表明,采用分期加高较一次建成的总工程费现值可节省1/3～1/2;以等库容分期加高的优化计算较为简单,且与等高度分期加高的优化计算结果相近;无约束分期加高变量较多,需采用非线性规划方法进行优化,计算过程较复杂,其计算结果最优;在分期加高中,使各次新增库容逐渐增大,有利于降低总工程费用现值.     

施用钢渣对土壤和水稻植株中硅、铁、锰的影响

盆栽试验结果表明:施用钢渣降低了土壤水溶态硅、DTPA-Fe、DTPA-Mn的含量,其效果随钢渣用量的增加而更为显著。但在钢渣用量一定时,却以中等粒度(60目)处理降低程度最大。施用钢渣还可以提高水稻植株体内硅的含量,降低水稻植株体内铁和锰的含量,其效果随钢渣用量增加或粒度变细而更为明显。     

土壤矿物钾的释放及其在植物营养中的意义

本文对土壤钾的形态、矿物钾释放的影响因素及钾的固定、土壤含钾矿物与土壤供钾能力间关系、矿物钾的植物有效性进行了综述 ,表明土壤钾素从有效性的角度进行划分时 ,不能仅把速效性钾和1mol/L热硝酸提取的缓效性钾列为作物吸钾的主要来源 ,而忽视矿物钾的作用 .     

Species composition of arbuscular mycorrhizal fungi in two mountain meadows with differing management types and levels of plant biodiversity

Species composition of arbuscular mycorrhizal fungi (AMF) was analysed in two differently managed mountain grasslands in Thuringia (Germany). Arbuscular mycorrhizal fungi were studied in the roots of 18 dominant plant species from a total of 56 (32%). Additionally, spores of AMF were isolated from soil samples. Arbuscular mycorrhizal fungi species composition was analysed based on 96 sequences of the internal transcribed spacer of the nuclear ribosomal DNA, 72 originated from mycorrhizal roots, and 24 originated from AMF spores. Phylogenetic analyses revealed a total of 19 AMF species representing all genera of the Glomeromycota except Scutellospora and Pacispora. Despite a different farming intensity, resulting in remarkable differences concerning their plant species diversity (27 against 43 plant species), the diversity of AMF was found to be similar with 11 species on the intensively farmed meadow and ten species on the extensively farmed one. Nevertheless, species composition between both sites was clearly different. It thus seems likely that the AMF species composition, but not necessarily the species number, is related to above ground plant biodiversity in the system under study.     

Molecular characterization and PCR detection of a nitrogen-fixing Pseudomonas strain promoting rice growth

Nitrogen-fixing plant growth-promoting rhizobacteria (PGPR) from the genus Pseudomonas have received little attention so far. In the present study, a nitrogen-fixing phytohormone-producing bacterial isolate from kallar grass (strain K1) was identified as Pseudomonas sp. by rrs (16S ribosomal RNA gene) sequence analysis. rrs identity level was high with an uncharacterized marine bacterium (99%), Pseudomonas sp. PCP2 (98%), uncultured bacteria (98%), and Pseudomonas alcaligenes (97%). Partial nifH gene amplified from strain K1 showed 93% and 91% sequence similarities to those of Azotobacter chroococcum and Pseudomonas stutzeri, respectively. The effect of Pseudomonas strain K1 on rice varieties Super Basmati and Basmati 385 was compared with those of three non-Pseudomonas nitrogen-fixing PGPR (Azospirillum brasilense strain Wb3, Azospirillum lipoferum strain N4 and Zoogloea strain Ky1) used as single-strain inoculants. Pseudomonas sp. K1 was detected in the rhizosphere of inoculated plants by enrichment culture in nitrogen-free growth medium, which was followed by observation under the microscope as well as by PCR using a rrs-specific primer. For both rice varieties, an increase in shoot biomass and/or grain yield over that of noninoculated control plants was recorded in each inoculated treatment. The effect of Pseudomonas strain K1 on grain yield was comparable to those of A. brasilense Wb3 and Zoogloea sp. Ky1 for both rice varieties. These results show that nitrogen-fixing pseudomonads deserve attention as potential PGPR inoculants for rice.     

氮磷钾互作效应对甘草黄酮含量影响的初步研究

研究结果表明,宁夏红寺堡扬黄新灌区沙壤灰钙土氮、磷、钾对甘草总黄酮含量影响大小为:施磷(负效应)大于施氮(正效应)大于施钾(负效应);因子互作效应对甘草总黄酮含量影响大小顺序为P×K>N×P>N×K;红寺堡扬黄新灌区沙壤灰钙土人工栽培甘草总黄酮含量≥5.0%以上的农艺措施为:施纯N 119.6~138.8kghm-2、施纯P2O5153.2~178.5 kghm-2、施纯K2O 90.9~105.6 kghm-2。     

Correlation between earthworms and plant litter decomposition in a tropical wet forest of Puerto Rico

Earthworms are recognized to play an important role in the decomposition of organic materials. To test the use of earthworms as an indicator of plant litter decomposition, we examined the abundance and biomass of earthworms in relation to plant litter decomposition in a tropical wet forest of Puerto Rico. We collected earthworms at 0–0.1 m and 0.1–0.25 m soil depths from upland and riparian sites that represent the natural variation in soils and decomposition rates within the forest. Earthworms were hand-sorted and weighed for both fresh and dry biomass. Earthworms were dominated by the exotic endogeic species Pontoscolex corethrurus Müller; they were more abundant, and had higher biomasses in the upland than in riparian sites of the forest. Plant leaf litter decomposed faster in the upland than riparian sites. We found that earthworm abundance in the upper 0.1 m of the soil profile positively correlated with decomposition rate of plant leaf litter. Ground litter removal had no effect on the abundance or biomass of endogeic earthworms. Our data suggest that earthworms can be used to predict decomposition rates of plant litter in the tropical wet forest, and that the decomposition of aboveground plant litter has little influence on the abundance and biomass of endogeic earthworms.     

近150年北京春季物候对气候变化的响应

对北京春季6种物候的计算得出其代表性指标一山桃始花期，并分析物候对气温变化的响应模式。根据气温变化态势，将历史时期气温和物候资料划分为4个时间段，并采用u检验论证时间段间具有显著性差异，分析物候期对气温变化的响应模式和机制，得出：物候期的提前与推迟对温度的增高与降低的响应是非线性的，在同等增、降温幅度下，因降温而导致的物候期推迟幅度较因增温而导致物候期提前幅度小；平均气温增高1℃，北京春季物候期提前2．8—3．6d。并估算了未来北京春季物候变化趋势。