Pisolithus tinctorius fails to improve plantation performance of inoculated conifers in southwestern Oregon

Bare-root stock of Douglas-fir, lodgepole pine, white fir, and grand fir, inoculated with Pisolithus tinctorius and handled by standard nursery, cold storage and planting practices performed no better than stock which was not intentionally inoculated but which had formed mycorrhizae with indigenous, nursery fungi on a variety of different outplanting sites in southwestern Oregon. Climate, planting sites and nursery practices in the Pacific Northwest differ drastically from those in the southeastern United States, where P. tinctorius has increased plantation survival and growth. Before P. tinctorius can be recommended for nursery inoculation in the Pacific Northwest, its reaction to each phase of the nursery and planting process needs to be carefully analyzed to determine where the problems lie.This article was written and prepared by US Government employees on official time, and is therefore in the public domain.     

新银合欢施石灰与根瘤菌接种效应的研究

新银合欢(Leucaena leucocephala cv.Salvador)又叫萨尔瓦多银合欢,是热带低地湿润地区树种,在适生条件下非常速生,是优良的多用途树种之一。 在自然条件下,新银合欢在中性和碱性土壤上生长良好,而适于该树生长的热带、亚热带气候区,土壤酸化现象日趋严重。许多国家试用施石灰、施肥、根瘤菌接种以及选种等     

酒糟厌氧消化产甲烷特性与微生物菌群结构分析

酒糟是酿酒生产过程中的副产物,以酒糟为研究对象,分别考察不同底物总固体（Total solid, TS）质量分数（0.5%、1%、1.5%、2%）、接种比（接种污泥与酒糟的挥发性固体质量比）（0.25、0.5、0.75、1、1.5）和温度（25、37、50℃）条件下酒糟的厌氧消化产甲烷特性。结果表明,随着TS浓度的增加,产甲烷量逐渐增加, 2%TS条件下可获得最大的累计产沼气量（532.8mL/g）和产甲烷量（294.7mL/g）。接种比是影响厌氧消化系统的重要因素,随着接种比的增大,系统累计产沼气和产甲烷量呈先增加后减少的趋势,在接种比为0.25和0.5的条件下,系统发生崩溃,并未产甲烷,当接种比为1时,获得最大的累计产沼气和甲烷量。当TS质量分数为2%、接种比为1.5、发酵温度为50℃的条件下,获得最大的累计产沼气量为559.4mL/g,相较37℃条件下提高了10.2%,而获得的累计产甲烷量为284.0mL/g,相较37℃并未显著提升（P>0.05）。利用修正的Gompertz模型进行产气动力学分析后,发现TS质量分数越高、接种比越大、温度越高,产甲烷迟滞期越短。同时对不同温度反应体系中的微生物群落进行了分析,发现Bacteroidetes和 Firmicutes为优势菌门,随着温度的升高,产甲烷菌逐渐由氢营养型代替乙酸营养型。因此, TS浓度、接种比和温度是厌氧发酵重要因素,初步确定2%TS、接种比为1的中温酒糟厌氧发酵产甲烷性能相对较好。     

循环流水水产养殖系统接种商品硝化菌的试验研究

与接种成熟滤料等方法相比,利用商品硝化菌接种生物滤器来源广泛,操作简便。本文使用一种商业上已取得一定成功的商品硝化菌液接种循环流水水产养殖系统试验模型的生物滤器。试验开始时定期接人20mL菌液(细菌含量1.5×10~10CFU·mL~(-1))以处理400g饲料产生的4.27mg·L~(-1)氨氮(TAN),12d后TAN浓度低于0.05mg·L~(-1),20d后NO_2~- -N浓度降至同样水平。在为期30d的检验期中,系统内养殖2.15kg·m~(-3)淡水白鲳,日喂饲率2％。期间养殖池TAN最大值0.465mg-L~(-1),最小值0.393mg·L~(-1),平均值0.427±0.019mg·L~(-1);NO_2~- -N最大值0.062mg·L~(-1),最小值0.038mg·L~(-1),平均值0.052±0.007mg·L~(-1);EC呈线性上升状态,每周增加32.8 μs·cm~(-1);而pH呈线性下降,每周降低0.24。系统结束时生物滤器含细菌8.65×10~6CFU·mL~(-1),水质除TP超标外,其余指标均符合我国淡水水产养殖水质标准,在2.1％～1.4％的日喂饲率下淡水白鲳日增重为1.91g,饲料系数1.164。     

Fungal inoculation induces agarwood in young Aquilaria malaccensis trees in the nursery

Fungi are often used to induce agarwood inAquilariatrees. This study was conducted to evaluate the effect of several fungi on agarwood formation over time in youngAquilaria malaccensis （Lam.） trees. Typical changes in the length and light intensity of the resulting discoloration were observed after three and six month periods following inoculation. Wood samples were observed microscopically and classified into several light intensity groups. The discoloration length was meas-ured longitudinally. The duration after inoculation affected the mean of discoloration length： the 6-month old sample （1.70 cm） had a wider discoloration zone when compared to the 3-month old sample （1.17 cm）. When measuring the discoloration intensity, a positive relationship with time was perceived. Digital images, captured using a camera-equipped microscope, revealed that wood samples collected after six months appeared to be 1.8-times darker than after three months. We concluded that time, not the species of any of the tested fungi, had significant effect on discoloration length and intensity. Gas chromatography/mass spec-trometry （GCMS） analysis of the 6-month old sample yielded some important agarwood compounds such as benzylacetone, anisylacetone, guaiene and palustrol. This demonstrates that the tested fungi have the ability to induce agarwood formation in nurseryA. malaccensis trees.     

Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings

Nine isonitrogenous (35% crude protein approximately) and isocaloric (18.37 kJ g^?1) experimental diets (RLL20–BCFL40) were formulated with either raw or treated (inoculated with fish intestinal bacteria) Leucaena leucocephala leaf meal at 20%, 30% and 40% levels replacing other ingredients partially from a fish meal based reference diet (RD). Two specific strains of fish intestinal bacteria, Bacillus subtilis (isolated from Cyprinus carpio) and B. circulans (isolated from Oreochromis mossambicus) having extracellular cellulolytic and amylolytic activities, were used to inoculate Leucaena leaf meal for 15 days at 37°C. The crude fibre, cellulose and hemicellulose contents and the antinutritional factors, tannin, phytic acid and mimosine in the leaf meal decreased due to inoculation. However, free amino acids and fatty acids increased in the treated leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a RD. Both the inclusion level and type of Leucaena leaf meal in diets significantly affected the growth performance of rohu. Fish fed diets containing inoculated Leucaena leaf meal performed better in comparison with those with the RD. On the basis of growth response, feed conversion ratio, protein efficiency ratio and apparent net protein utilization, diet formulated with 30%Leucaena leaf meal inoculated with B. circulans resulted in the best performance of rohu fingerlings followed by diet with 40%B. subtilis inoculated Leucaena leaf meal. The apparent protein digestibility (APD) was better in fish fed diets containing B. circulans inoculated leaf meal. An increasing level of raw Leucaena leaf meal was associated with a decrease in the carcass protein content of rohu fingerlings. The activity of α‐amylase increased with the increasing level of treated leaf meal in diets. Cellulase activity increased with increasing level of inclusion of raw leaf meal, and was comparatively lower in fish fed diets with treated leaf meal. Activities of protease and lipase were higher in fish fed the RD. The results showed that it is possible to incorporate Leucaena leaf meal inoculated with enzyme‐producing fish intestinal bacteria in carp diets up to 40% level of inclusion.     

播后镇压和冬前灌溉对土壤条件和冬小麦生育特性的影响

为揭示冬灌和镇压的作用,以冬小麦品种石新828和石麦12号为材料,研究了冬灌和镇压措施对麦田土壤水热条件和冬小麦生育特性的影响。结果表明,与不冬灌相比,冬灌条件下,冬季和春季土壤含水量提高,土壤温度较稳定。与不镇压相比,镇压处理的冬前和起身期土壤含水量及冬前夜间土壤温度提高。冬灌处理冬小麦在越冬后各时期的总茎数、LAI、干物质积累量有所降低,开花期叶片光合速率显著下降,灌浆后期叶片SPAD值下降也较快,每穗总小穗数、结实小穗数、穗粒数和产量均减少。镇压使小麦开花前干物质积累量减少,但对成熟期干物质积累量影响不显著,因为开花后光合速率提高,叶片SPAD值下降较缓慢,开花后干物质积累较多。镇压处理成熟期的单位面积穗数降低,但由于穗粒数和千粒重提高,镇压与不镇压的产量差异不显著。从本研究结果看,冬灌是一项防冻保苗的有效措施,与播后镇压配合可减缓冬季低温和温度剧烈变化对小麦的不利影响。     

矮种椰子育苗方法研究

以‘文椰2号’、‘文椰3号’和‘文椰4号’成熟种果为材料,研究种果长宽比值、预处理方法对种果萌发率、萌发时间、幼苗生长的影响。结果表明:不同长宽比值对种果发芽率没有显著影响,但对萌发时间影响显著,对幼苗生长指标也有显著影响。不同预处理对部分品种的萌发时间和萌发率存在显著影响,部分生长指标与对照相比得到显著提高。     

Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): Presence of viral DNA and differentiation among populations

Plants collected in thirteen wild populations of Capsicum annuum from Northwest Mexico were tested for resistance to the pepper huasteco begomovirus (formerly subgroup III) (PHV) that is transmitted by the white fly Bemisia tabaci Genadius. Plants were inoculated using both grafting and biolistic methods. Presence of viral DNA was detected by dot blot hybridization and densitometry. Populations varied in their resistance to PHV. Plants of only two of the populations either did not develop disease symptoms or showed very light symptoms after inoculation. In some cases, symptoms appeared several days after inoculation. In plants of these populations viral DNA was detected by dot-blot hybridization assays but they appear to be a good source of resistance (symptomless) for use in breeding programmes. This revised version was published online in August 2006 with corrections to the Cover Date.