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91.
Characterization and application of monoclonal antibodies against white spot syndrome virus 总被引:1,自引:1,他引:1
Three hybridoma clones secreting monoclonal antibodies (MAbs) were produced from mouse myeloma and spleen cells immunized with white spot syndrome virus (WSSV) isolated and purified from Penaeus monodon (Fabricius), collected from north-eastern Taiwan. By sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), the protein profile of this isolate contained four major proteins with sizes of approximately 35 (VP35), 28 (VP28), 24 (VP24), and 19 kDa (VP19). Western blot analysis revealed that two MAbs (1D7 and 6E1) recognized epitopes on VP28 and one MAb (3E8) recognized an epitope on VP19. The MAb 6E1 isotyped to the IgG1 class was used in both an indirect immunofluorescence assay (IFA) and in an immunochemical staining protocol for successful identification and localization of WSSV in infected shrimp tissues. Antigenic similarity of isolates from Indonesia and Malaysia to the Taiwan isolate was illustrated by IFA with MAb 6E1. A MAb (2F6) which bound specifically to two shrimp proteins, 75 and 72 kDa, and reacted to the healthy and non-target tissues of WSSV in infected shrimp, such as hepatopancreas, is also described here and shows the necessity for specific identification of antibodies. 相似文献
92.
Sílvio Peixoto Greg Coman Stuart Arnold Peter Crocos & Nigel Preston 《Aquaculture Research》2005,36(7):666-673
Oocyte maturation and gonadosomatic index (GSI) of eyestalk ablated Penaeus monodon females collected from the wild and from two first‐generation domesticated lines were assessed. Frequency and diameter of the different oocytes, and the intensity of oocyte atresia, were compared among groups through histological assessments of the sections of the middle ovarian lobe. Digitized images from ovary sections were used to record the frequency and diameter of different oocyte types. Spawning performance of the three groups were expressed in terms of the percentage of females that spawned at least once (productive females), time from eyestalk ablation to first spawning (latency period) and the number of spawnings per female stocked. Final ovarian maturation was attained in all groups, as indicated by the presence of mature oocytes with cortical rods (cortical oocytes), dark‐green ovarian colour and high GSI values (5.83–6.86%). However, domesticated groups presented significant larger immature oocyte types (previtellogenic and yolky oocytes) and smaller cortical oocytes compared with wild females, indicating a reduced vitellogenic activity during final oocyte maturation. Additionally, the frequency of atresia was comparatively higher for both domesticated groups, which could be related to their inferior spawning performance. The implications of these results on the reproductive potential and development of domesticated P. monodon are discussed. 相似文献
93.
Fusarium incarnatum isolated from black tiger shrimp, Penaeus monodon Fabricius, with black gill disease cultured in Vietnam 总被引:1,自引:0,他引:1
Fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, Penaeus monodon, in every crop during 2000-2002 in Nghe An province, Vietnam. Infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. Detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal DNA, were made on the isolates. An artificial infection of kuruma prawn, Penaeus japonicus, using two selected isolates was also conducted and their pathogenicity determined. 相似文献
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96.
中国对虾幼体发育阶段氨基酸组成的研究 总被引:9,自引:0,他引:9
中国对虾幼体发育阶段氨基酸组成的研究马英杰,张志峰,廖承义,马爱军,孙谧(青岛海洋大学,青岛266003)(黄海水产研究所,青岛266071)关键词中国对虾,幼体发育,氨基酸THESTUDYONTHECOMPOSITIONOFAMINOACIDINL... 相似文献
97.
叙述了中国对虾亲虾(海捕亲虾与池养亲虾)在生殖周期中肝胰腺、中肠的消化酶活性及卵巢、肝胰腺、血淋巴生化成分的研究结果。蛋白酶、脂肪酶在卵巢成熟的Ⅳ期前活性较高,临产卵时(Ⅴ期)活性明显下降,而且海捕亲虾与池养亲虾具有相同的变化趋势;中国对虾卵巢、肝胰腺和血淋巴的蛋白质和脂肪含量随性腺发育有不同的变化规律,其中总蛋自在这几种组织中均随性腺发育含量增加,至Ⅳ期(肝胰腺)和Ⅴ期(卵巢、血淋巴)达到高峰值;卵巢的总脂和甘油三酯从发育Ⅰ期-Ⅴ期随着性腺指数的增长而有规律的上升,但肝胰腺脂肪含量在卵巢发育初期(Ⅰ期-Ⅱ期)未见明显变化,Ⅱ期以后,肝胰腺脂肪含量逐渐下降,当卵巢脂肪含量达到最高值时,肝胰腺脂肪含量正好下降到最低点。同时,还对中国对虾卵巢发育过程中蛋白质和脂肪的积累问题进行了分析和讨论。 相似文献
98.
99.
中国对虾往西北内陆咸水水域移植的生产性试养研究 总被引:8,自引:1,他引:8
本文介绍了通过采用引进河水、压碱降盐等改良水质的技术措施,使中国对虾首次成功地移植到内陆咸水水域。经过1个月的试养,中国对虾平均体长增长3.82 cm,平均体重为1.82 g。 相似文献
100.
Roshan Pais Malathi Shekar Indrani Karunasagar Iddya Karunasagar 《Aquaculture (Amsterdam, Netherlands)》2007,270(1-4):529-534
The giant fresh water prawn Macrobrachium rosenbergii is known to be highly tolerant to white spot syndrome virus (WSSV) infections when compared to the widely cultured marine tiger shrimp Penaeus monodon. At present, the exact mechanism of tolerance by M. rosenbergii to WSSV is not known. In this study, we attempt to study the effect of WSSV injections on the hemagglutination activity of the hemolymph serum of both P. monodon and M. rosenbergii and look for changes if any, on their hemolymph serum protein electrophoretic patterns. Our results show that M. rosenbergii had significantly (p < 0.05) higher hemagglutinating activity against mouse erythrocytes when compared to P. monodon. As the infection progressed to 48 h there was a further increase (p < 0.05) in the hemagglutination activity in M. rosenbergii, while it decreased in P. monodon. 12% SDS-PAGE analysis of the hemolymph serum of M. rosenbergii infected with WSSV did not show any new protein bands, whereas few bands with decreased intensity was observed in moribund P. monodon where the hemagglutinating activity was also observed to be decreased. The results indicate that hemolymph hemagglutinin levels are modulated in crustaceans as a response to viral infections. 相似文献