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[目的]研究花叶玉簪的组培技术。[方法]以花叶玉簪为研究对象,采用浓度75%的酒精进行消毒,并以MS为基本培养基,添加不同浓度植物生长调节剂6-BA和NAA进行增殖培养和生根培养,观察生长状况。[结果]MS+4.0mg/L6-BA+0.1mg/LNAA增殖效果最好,苗生长健壮。最佳生根培养基为:1/2MS+0.1mg/LNAA+0.3%活性炭;20d后平均根长可达3cm,且根系比较粗壮,移栽后易成活。幼苗移栽时以蛭石:珍珠岩(5:1,W/W)、泥炭土:珍珠岩:蛭石(3:1:1,W/W/W)或腐叶土:蛭石(3:1,W/W)的成活率较高,小苗生长健壮。[结论]采用组培方法繁殖花叶玉簪,虽然具有繁殖系数高,时间短等优点。 相似文献
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G. RAGHAVAN 《Aquaculture Nutrition》2011,17(3):327-331
Filtration rate and ingestion rate of different stages of Paphia malabarica larvae (D‐shape, (80 μm), Umbo (120 μm) and veliger (180 μm)) were determined in relation to feeding on various micro algae. The micro algae tested were Nannochloropsis salina, Isochrysis aff. galbana, Dicrateria inornata, Tetraselmis gracilis and Chaetoceros calcitrans at 5 or 10 × 103 cells mL?1. Both filtration and ingestion rate of micro algae tested were increased with increasing larval size; however, at all larval stages, C. calcitrans resulted in lower filtration and ingestion rate. Of the algal diets tested, P. malabarica larvae showed greatest filtration rate and ingestion rate with N. salina. Maximum filtration rate for N. salina was 15.7, 26.3 and 33.9 μL h?1 and highest ingest rate was 15, 92 and 177 cells larva?1 h?1 in D shape larvae, umbo and pediveliger, respectively. Filtration rate and ingestion rate of N. salina were always higher than those other algal species tested because of its small cell size (2 μm). 相似文献
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波叶红果树种子萌发特性 总被引:2,自引:1,他引:1
以波叶红果树Stranvaesia davidiana var.undulata种子为试验材料,通过设置不同的果实储藏方式、不同的萌发温度和光照条件,对其种子萌发特性进行研究。结果表明:用室温干藏,低温(4℃)干藏,自然低温湿砂层积处理波叶红果树果实后,种子发芽率达极显著差异(P〈0.01),以自然低温湿砂层积处理种子发芽率最高,达41.3%;在10,15,20,25,30,15/10,25/15,30/20℃等8个不同温度条件下,种子发芽率达显著(P〈0.05)和极显著差异(P〈0.01),在25℃条件下种子发芽率最高,达45.3%;在全黑暗、光照12h(10301x)和光照24h(10301x)等3个条件下,种子发芽率无显著差异(P〈0.05),说明波叶红果树种子为光不敏感种子或光中性种子。因此,波叶红果树果实经自然低温湿砂层积处理后,在25℃条件下,有利于提高种子发芽率,光照不是其种子萌发的必需条件。 相似文献
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Biochemical compositions were determined for eggs, D-shaped larvae, umbo larvae and pediveliger of the short neck clam Paphia malabarica Chemnitz. Spawned eggs were composed of 63.2% protein, 25.4% lipid and 11.4% carbohydrate. After 48 h of embryogenesis, 2.6% of the protein, 11.8% of the lipid and 3.2% of carbohydrate mass had been lost, providing 20.5%, 75.4% and 4.1% of the total energy expenditure of 0.2147 mJ embryo−1 . During 48 h of metamorphosis, lipid was utilized first, followed by a heavy consumption of protein; protein, lipid and carbohydrate lost 23.8%, 50.2% and 32.5% of their mass respectively. Protein and lipid supplied a comparable amount of energy for metamorphosis, 34.2% and 55.2%, respectively, whereas, carbohydrate contributed only 10.6% to the 2.733 mJ larva−l metamorphic energy expenditure. 相似文献
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巴菲蛤壳脂溶性成分的气相色谱-质谱联用分析 总被引:1,自引:0,他引:1
[目的]为寻找巴菲蛤壳脂溶性成分的新用途提供理论支持。[方法]以氯仿为溶剂回流提取巴菲蛤壳中的脂溶性成分,利用气相色谱-质谱联用仪对提得物质进行分离和鉴定。采用色谱峰面积归一化法确定脂肪酸各组成成分的相对含量。[结果]从巴菲蛤壳的脂溶性成分中分离鉴定出了44种成分,包括5种羧酸类化合物,占总量的16.44%,其中含量最高的是戊酸(8.43%)和4-氧基苯甲酸(3.59%);28种烃类化合物,占总量的40.87%,其中含量最高的是稠环芳烃萘烷(8.43%)、脂肪烃二叔十二烷基二十一烷(3.53%)、环烃环己烷(3.46%)和烯烃5-二十烯(0.45%);还有4种含溴化合物、2种硅氧烷类化合物、2种酮类化合物、2种含硫化合物和1种含硼化合物。[结论]烃类化合物可确定为巴菲蛤壳脂溶性成分中的主要成分。 相似文献
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东山湾巴非蛤养殖区海水、沉积物重金属的含量分布与潜在生态危害评价 总被引:4,自引:1,他引:4
2003年5月、8月和10月对东山湾巴非蛤养殖区环境状况进行调查,根据调查资料,分析了巴非蛤养殖区海水、表层沉积物中Cu、Pb、Cd、Zn的含量分布,并进行生态环境质量评价及潜在生态危害评价.结果表明:东山湾巴非蛤养殖区海水中Cu、Pb、Cd、Zn的平均含量分别为1.75 μg.L-1,1.69 μg.L-1,0.02 μg.L-1,2.84 μg.L-1,其季节变化从高到低依次为10月>8月>5月;沉积物中Cu、Pb、Cd、Zn的平均含量分别为14.0 mg.kg-1、34.8 mg.kg-1、0.031 mg.kg-1、97.5 mg.kg-1.生态质量评价结果表明,养殖区海水重金属的含量符合第二类海水水质标准要求,处于清洁水平.养殖区沉积物重金属的含量符合第一类海洋沉积物质量标准,属于较清洁水平.生态危害评价表明,Pb和Cd的潜在生态危害系数大于Cu和Zn,但整个养殖区沉积物中重金属的潜在危害属于轻微,养殖环境良好. 相似文献
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