Metabolism, uptake and translocation of 14C-paraquat in resistant and susceptible biotypes of Crassocephalum crepidioides (Benth.) S. Moore

The metabolism, uptake and translocation of paraquat in resistant (R) and susceptible (S) biotypes of Crassocephalum crepidioides (Benth.) S. Moore (redflower ragleaf) at the 10-leaf stage was investigated. A study on the properties of leaf surface was carried out to examine the relationship between leaf surface characters and paraquat absorption. The extractable paraquat was not metabolized by the leaf tissue of either the resistant or susceptible biotypes. Differential metabolism, therefore, does not appear to play a role in the mechanism of resistance. Both biotypes did not show any significant difference in the amount of cuticle and trichome densities. Furthermore, both biotypes are identical in the structure of stomata, trichomes and epicuticular wax. The results of the leaf surface studies are in agreement with the findings of the uptake study. Both biotypes demonstrated no significant difference in absorption between the resistant and susceptible biotypes. However, 10% of the absorbed ¹⁴C-paraquat into the S biotype was translocated basipetally, but not in the R biotype. The results of this study suggest that in C. crepidioides , differential translocation may contribute to the mechanism of resistance at the 10-leaf stage.     

局部灌水方式对冬小麦产量与水分利用的影响

以根系对土壤水分的吸收利用规律、土壤养分在土层中的分布规律和农田土壤蒸发特征为基础,研究局部不同节水高产灌水方式,是解决水肥异位问题和实现“浇作物”的新尝试。2000～2002年连续两年的试验结果表明,测坑内灌溉5水的处理与筒栽灌溉7水的处理有最高的产量和千粒重,适宜的灌水量是30mm。提高作物产量和水分利用效率的灌溉方式应该是灌水集中在作物主要根系分布层,实施高频局部灌溉。     

Ameliorative effects of inoculation with the plant growth-promoting rhizobacterium Pseudomonas sp. DW1 on growth of eggplant (Solanum melongena L.) seedlings under salt stress

The objective of this work was to evaluate the effect of inoculation with the plant growth-promoting rhizobacterium Pseudomonas sp. DW1 on eggplant (Solanum melongena L.) growth, mineral uptake and activities of the antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of plant leaves under salinity stress. The study was conducted in pot experiments using eggplant (S. melongena L., cv. Yinjia) and a coastal soil. The NaCl concentration of the coastal soil was 0.57 g (kg soil)⁻¹. Four NaCl levels were tested: 0.57, 1.0, 2.0, and 3.0 g NaCl (kg soil)⁻¹, by adding NaCl to soil, respectively. Pseudomonas-inoculated seeds had an increase in the germination percentage over its non-inoculated seeds under salinity. Salinity negatively affected growth of eggplant; however, plants inoculated with Pseudomonas sp. DW1 grew to a significantly greater extent than plants that were not treated with this bacterium. Salinity significantly decreased K⁺ concentration, increased Na⁺ concentration, and did not significantly decrease Ca²⁺ content in shoots of eggplants. Inoculating with Pseudomonas sp. DW1 increased shoot Ca²⁺ of eggplant compared to the non-inoculating eggplant plants under salinity. Inoculating treatments with Pseudomonas sp. DW1 had no effect on shoot Na⁺ concentration in 0.57 and 1 g (kg soil)⁻¹ NaCl, but there were significant decreases in inoculated treatments than in non-inoculated ones at 2 and 3 g (kg soil)⁻¹ NaCl. Salinity decreased SOD activities and increased POD activities, and inoculated Pseudomonas sp. DW1 had an increase effect on SOD activity in the leaves of eggplants. Alteration of mineral uptake and increase in the antioxidant enzyme activities may be two mechanisms for the alleviation of salt stress. Based on the results of the experiment reported herein, the use of the plant growth-promoting rhizobacterium treatment may provide a means of facilitating plant growth under salt stress.     

基于无人机遥感影像的冬小麦氮素监测

精准氮素管理是一项提高作物氮肥利用效率的有效策略,利用无人机遥感技术精确估测小麦氮素状况是必要的。试验在山东省乐陵市科技小院实验基地进行,利用八旋翼无人机搭载Mini-MCA多光谱相机于2016年获取冬小麦4个关键生育时期(返青期、拔节期、孕穗期、扬花期)冠层多光谱数据,同步获取地上部植株样品并测定其生物量、吸氮量、氮营养指数,及成熟期籽粒产量,根据各关键生育期与全生育期分别构建植被指数与农学参数回归分析模型,评估基于无人机遥感影像的冬小麦氮素营养诊断潜力。结果表明:基于无人机遥感影像能够较好地估测冬小麦氮素指标(R2为0.45~0.96),决定系数随着生育期推移而逐渐增大。拔节期、孕穗期和扬花期估产效果接近且具有很好的估测能力,扬花期DATT幂函数模型对小麦氮营养指数的解释能力最强(R2=0.95)。因此,以多旋翼无人机为平台同步搭载多光谱相机对冬小麦有较好的氮素诊断潜力,可利用估测结果指导精准氮肥管理。     

High potassium to magnesium ratio affected the growth and magnesium uptake of three tomato (Solanum lycopersicum L.) cultivars

Potassium(K) and magnesium(Mg) levels and their balances are two factors affecting the growth of plant. However, the responses of different crop cultivars to K/Mg ratios are less clear. This study was aimed at assessing the different responses of tomato(Solanum Lycopersicum L.) cultivars to the different K/Mg supply ratios. Three tomato cultivars(Zhongza 9(ZZ), Gailiangmaofen(MF), and Jinpengchaoguan(JP)) were grown in pots with three different K~+/Mg~(2+) ratios(4:0, 4:1 and 8:1, represented by K/Mg_(4:0), K/Mg_(4:1), and K/Mg_(8:1), respectively). Compared with K/Mg_(4:1) treatment, the leaf chlorophyll content, net photosynthetic rate, and total biomass of tomato seedlings under K/Mg_(4:0) treatments were decreased by 69.7, 89.1, and 53.1%, respectively. The Mg deficiency symptoms were observed when the Mg content in shoot became lower than 4 mg g~(–1) DW. Compared with K/Mg_(4:1) treatment, total biomass of tomato seedlings of K/Mg_(8:1) treatment was decreased by 21.6%; the shoot and root Mg contents were decreased by 10.4 and 21.8%, respectively; and Mg uptake of tomato was reduced by 34.1%. There were significant differences in biomass and Mg uptake for the three cultivars between the different K~+/Mg~(2+) treatments. The Mg uptake of the three different cultivars ranked as ZZJPMF under Mg deficiency and high K condition. In conclusion, the growth and Mg uptake and allocation of tomato were influenced significantly by imbalance K and Mg supply. JP and ZZ were the cultivars with the highest efficiency in Mg uptake.     

Soil Colloidal P Release Potentials under Various Polyacrylamide Addition Levels

Anionic polyacrylamide (PAM) can prevent soil erosion, but its effect on fine particulate phosphorus (P), such as colloidal P, has not been thoroughly examined. The effects of PAM on the release potentials of water‐dispersible colloids (WDC) and total P, molybdenum‐reactive P (MRP), and molybdenum‐unreactive P (MUP) in the colloidal and truly dissolved phases (i.e., TP_coll, MRP_coll, MUP_coll, TP_truly, MRP_truly, and MUP_truly) from six soils across South China were tested in this study. The results showed that the release potentials of TP_coll in the control treatments were 6·9–46·1 mg kg⁻¹ and generally highest in sandy loam soil. Following low (12·5 kg ha⁻¹), middle (25 kg ha⁻¹), and high (50 kg ha⁻¹) levels of PAM application, the release potential of TP_coll decreased by 41·7, 63·2, and 77·4% compared to the control group, respectively. Additionally, PAM may trigger MRP_coll and TP_truly releases in sandy loam and/or silt soils, and for most soils, MRP_truly and MUP_truly showed the highest release potentials at middle or high PAM levels. A significant PAM application level by soil site interaction for the release potentials of WDC and colloidal P was observed. Multiple linear regression showed that the PAM rate combined with soil sand content can successfully predict the release potentials of WDC (R² = 0·552, p  < 0·001) and TP_coll (R² = 0·738, p  < 0·001). Our results suggest that PAM can effectively reduce the loss of soil colloids and colloidal P, while its effects are related to both application level and soil texture. Copyright © 2017 John Wiley & Sons, Ltd.     

Phosphorus availability of sewage sludge‐based fertilizers determined by the diffusive gradients in thin films (DGT) technique

The plant‐availability of phosphorus (P) in fertilizers and soil can strongly influence the yield of agricultural crops. However, there are no methods to efficiently and satisfactorily analyze the plant‐availability of P in sewage sludge‐based P fertilizers except by undertaking time‐consuming and complex pot or field experiments. We employed the diffusive gradients in thin films (DGT) technique to quantify the plant P availability of various types of P fertilizers with a novel focus on sewage sludge‐based P fertilizers. Mixtures of fertilizer and soil were incubated for 3 weeks at 60% water holding capacity. DGT devices were deployed at the beginning of the incubation and again after 1, 2, and 3 weeks. Two weeks of incubation were sufficient for the formation of plant‐available P in the fertilizer/soil mixtures. In a pot experiment, the DGT technique predicted maize (Zea mays L.) biomass yield and P uptake significantly more accurately than standard chemical extraction tests for P fertilizers (e.g ., water, citric acid, and neutral ammonium citrate). Therefore, the DGT technique can be recommended as a reliable and robust method to screen the performance of different types of sewage sludge‐based P fertilizers for maize cultivation minimizing the need for time‐consuming and costly pot or field experiments.     

Identification of mutations in the para sodium channel of Bemisia tabaci from Crete, associated with resistance to pyrethroids

We investigated the mechanisms of resistance to α-cypermethrin in a Q biotype, highly resistant Bemisia tabaci strain (GRMAL-RP) isolated from Crete. Cytochrome P450-dependent monoxygenase activity with the substrate ethoxycoumarin, and carboxylesterase activity with the substrates α-naphthyl-acetate, β-naphthyl-acetate, and para-nitrophenol acetate were substantially elevated in the GRMAL-RP, compared to the susceptible SUD-S strain, while glutathione-S-transferase activity with the substrate 1-chloro-2,4-dinitrobenzene was not different. The metabolic inhibitors piperonyl butoxide and S,S,S-tributyl phosphorotrithioate synergised cypermethrin toxicity in the GRMAL-RP strain, however, mortality was still lower than that of the susceptible strain, indicating the presence of an additional resistance mechanism. Analysis of the sequence of the IIS4-IIS6 region of the para sodium channel gene of the GRMAL-RP strain revealed two amino acid replacements compared to that of the SUD-S susceptible strain. One is the leucine to isoleucine substitution at position 925 (L925I) previously implicated in B. tabaci pyrethroid resistance and the other is a novel kdr resistant mutation for B. tabaci, a threonine to valine substitution at position 929 (T929V). Genotype analysis showed that the L925I and T929V were present in all GRMAL-RP males tested, at an approximately 1:1 frequency, but never in combination in the same haplotype.