不同施氮量对水稻氮素吸收与分配的影响

运用15N示踪法研究了不同施氮量对两个品种水稻（4007和武运粳15）干物质积累量与其对15N吸收及分配的影响。结果表明,当施氮量超过N 150 kg/hm2时, 两个品种水稻子粒产量均不再显著增加。4007在4个施氮量下(N 100,150,200和 250 kg/hm2)分别比无氮区增产22.3%,36.9%,43.2%和38.1%;武运粳15分别增产10.6%,18.8%,27.1%和21.5%。同一施氮量下,4007子粒中15N累积量显著高于武运粳15,但茎叶和根中没有差异。增加施氮量降低了15N在水稻子粒中的分配比例,但提高了茎叶中15N的分配比例。15N在根中的分配比例不受施氮量和品种的影响。研究结果还表明,同一施氮量下,4007对肥料氮的总体利用率要比武运粳15高3～6个百分点。     

潮土小麦和玉米Olsen-P农学阈值及其差异分析

【目的】磷农学阈值是指导不同作物磷肥用量并获取最佳经济产量的重要依据,然而,不同地区不同的耕作制度、土壤类型、作物种类、pH、温湿度条件下,作物的磷农学阈值不同。明确小麦–玉米轮作体系下,典型潮土区小麦和玉米的磷农学阈值,并分析其差异。【方法】本研究基于“国家潮土土壤肥力与肥料效益长期监测站”25年的定位试验,选取氮、钾肥施用充足和磷肥用量不同的NK (不施磷肥)、NPK (施用氮磷钾化肥)、NPKM (氮磷钾化肥和有机肥配施)、1.5NPKM (高量氮磷钾化肥配施有机肥)、NPKS (氮磷钾化肥与玉米秸秆还田配施) 5个处理的试验数据,使用米切里西指数模型 (Mitscherlich exponential model) 拟合小麦和玉米的Olsen-P农学阈值,并通过对比不同土壤磷水平下两种作物的磷吸收利用特性,分析其阈值不同的原因。【结果】获得最大相对产量的95%时,潮土区小麦Olsen-P农学阈值为13.1 mg/kg,玉米Olsen-P农学阈值为7.5 mg/kg。玉米Olsen-P农学阈值低于小麦主要原因:1) 土壤磷水平较低时,小麦对磷缺乏更为敏感,而玉米可保持相对较强的吸磷能力,25年不施磷处理玉米吸磷量是小麦的1.4倍;2) 土壤Olsen-P含量达到玉米阈值,而未能达到小麦阈值时,可保障玉米籽粒、茎秆及小麦籽粒正常生长对磷的需求,但小麦茎秆磷浓度仅能达到相对最大磷浓度的68.9%,严重影响了小麦的正常生长和获取较高产量的能力;土壤Olsen-P含量提高到小麦阈值后,小麦茎秆磷浓度提高到相对最大磷浓度的80.5%以上,进而可保障小麦获得较高的产量;3) 土壤磷素养分充足时,小麦对磷的吸收量大于玉米,且主要是由于小麦茎秆磷浓度和吸磷量随土壤Olsen-P含量的增加而大幅度增加。【结论】小麦和玉米作为典型潮土区两种重要的粮食作物,Olsen-P农学阈值分别为13.1和7.5 mg/kg。由于两种作物的生理特性不同,小麦对磷素的吸收利用率较低,茎秆需要较高的土壤磷浓度维持正常生长,产量形成对磷养分需求更大。因此,小麦–玉米轮作体系下,小麦的磷农学阈值更高,小麦季所需土壤磷供应量大于玉米季。为增强磷肥利用效率,减少磷肥投入量和土壤中磷素的过量累积,玉米季磷肥使用量应适当小于小麦季。当土壤Olsen-P水平高于作物磷农学阈值后,减少或短时间停止施用磷肥并不会对作物产量有明显影响。     

Composts Vary in Their Effect on Soil P Pools and P Uptake by Wheat

With diminishing world reserves of phosphorus (P) deposits and rising fertilizer prices, it is important to find alternate sources of P for crops. The aim of this research was to evaluate the effect of four different composts C1 (animal manure and straw), C2 (garden waste), C3 (wood chips and bark), and C4 (kitchen waste) on soil P pools and P uptake by wheat on 14, 28, and 72 days after compost application. The composts were applied as a 2.5 cm thick layer on the soil surface. During sampling, only the soil underlying compost was sampled. Soil pH and total organic carbon were not affected by the amendments. Soil respiration was significantly higher in compost-amended soils compared with the unamended soil except with C4 on day 72. Addition of composts increased plant growth, and P uptake being highest on day 72 with C1 and C4. With little effect on available P concentration on day 14, there was a conversion of organic P into inorganic P in the compost treatments suggesting net mineralization of organic P on day 28. On day 72, the concentrations of the less labile P forms were higher in the compost treatments compared with the unamended suggesting precipitation and fixation as well as synthesis of organic P. This study showed that mulching with composts having high available and total P concentrations can provide plants with P and also increase soil P concentrations which could reduce the fertilizer requirement for the following crop.     

Acidulated activation of phosphate rock enhances release,lateral transport and uptake of phosphorus and trace metals upon direct-soil application

Phosphate rock (PR) was activated via acidulation with HCl, EDTA, and oxalic acid to enhance its reactivity. The release, lateral transport, and uptake of phosphorus (P) along with trace metals from pristine and activated PRs were investigated in a soil micro-block system over a period of 27 days, using wheat (Triticum aestivum L.) plants. Significantly (p < 0.05) higher amounts of available soil P, Fe, Mn, and Zn were released from all the PRs after application to soil within first 9 days of seedling transplantation, while the release of other trace metals (Cd, Co, Cr, Cu, Ni, and Pb) was minimal (<1.2 mg kg^?1). On cumulative basis, APR-O (oxalic acid activated PR) was the most efficient amendment releasing 164% more available P, followed by APR-E (EDTA activated PR) releasing 130% more available P, compared to the pristine PR. Similar results were also observed in the release of available Fe, Mn, Zn, and other trace metals. The highest diffusive mass fluxes for available P, Mn, Fe, and Zn in soil were observed after 3 days of seedling transplantation, which reduced subsequently. The uptake of P, Fe, Mn, and Zn by wheat plants was increased by 394%, 715%, 92%, and 91%, respectively, in APR-O application compared to the pristine PR, while it was increased by 280%, 188%, 16%, and 27%, respectively, in APR-E application compared to the pristine PR. Subsequently, APR-O and APR-E amendments resulted in enhanced shoot lengths, root lengths, shoot dry matter, and root dry matter contents of wheat plants. Hence, it was concluded that activation of PR with oxalic acid and EDTA prior to direct soil application may enhance the reactivity of PR and could serve as a cost-effect fertilization strategy for higher wheat crop production.     

旱地高产小麦品种籽粒锌含量差异与氮磷钾吸收利用的关系

【目的】明确旱地条件下高产小麦品种籽粒锌含量差异与氮磷钾吸收利用的关系,为品种选育和科学施肥提供依据。【方法】于2013—2016年连续三年在黄土高原旱地进行田间试验,试验采用裂区设计,主处理为不施肥(CK)和施肥(NP),副处理为来自我国主要麦区的123个品种。施肥处理为N 150 kg/hm^2 (尿素,含N46%)、P_2O_5 100 kg/hm^2 (过磷酸钙,含P_2O_5 16%)。成熟期在每个品种中间2行随机抽取30穗小麦,连根拔起后,从根茎结合处剪断弃去根系,分为茎、叶、颖壳(含穗轴)和籽粒,称风干重。分析了样品中氮、磷、钾、锌含量,计算了养分的吸收量及转移量。【结果】施肥条件下高产小麦品种籽粒锌含量存在显著差异,高锌品种比低锌品种显著高54%。高锌品种的籽粒氮、磷含量分别比低锌品种显著高9%、7%,钾含量无显著差异,施肥使两组品种的氮含量显著提高,磷钾含量降低。高产高锌品种具有更高的籽粒和地上部氮、磷吸收能力,钾吸收能力与低锌品种相比无显著差异,施肥可使高锌品种的氮磷钾吸收量增幅高于低锌品种;两组品种间的氮、磷转移能力无显著差异,而高锌品种的钾转移能力较低,且两组品种的氮磷钾转移能力因施肥降低幅度一致。【结论】旱地条件下土壤养分供应充足时,高产高锌小麦品种的氮磷吸收能力强,钾转移能力弱,籽粒氮磷含量高,与低锌品种相比钾含量无显著差异。通过品种选育可同时提高旱地高产小麦籽粒锌和蛋白质含量,并提高磷含量。考虑到磷含量高时会降低籽粒锌的生物有效性,生产中通过施肥措施,适当调控磷肥,增加氮肥,在提高小麦籽粒氮锌含量的同时提高籽粒锌的生物有效性。     

Effects of nitrogen feeding for extraradical mycelium of Rhizophagus irregularis maize symbiosis incorporated with phosphorus availability

In terrestrial ecosystems, plants are frequently in symbiosis with arbuscular mycorrhizal fungi (AMF) with mineral nutrients and photosynthesis carbon exchanges in between. This research sought to identify the effects of phosphorus (P) levels on the nitrogen (N) uptake via extraradical mycelium (ERM) and the mycorrhizal growth response (MGR) of maize plants within the AMF symbiosis. Pots were separated into root compartments and hyphae compartments (HCs) with two layers of a 30‐μm mesh membrane and an air gap in between, where only hyphae could pass through, to avoid both N diffusion and root growth effects. Maize plants were inoculated with Rhizophagus irregularis with different N fertilization in HCs under two different P fertilization levels. Our results indicated that a strong increase in MGR with low‐P fertilization. The same tendency was not observed with high‐P fertilization, although both had a large increase in P concentration as a potential source of growth in shoot tissue of mycorrhizal plants. Substantial effects (10.5% more N) were observed in the case of high‐P availability for the host plants from ERM fed with N, whereas under low‐P conditions ERM may prioritize P uptake rather than N uptake. The AM fungi increase the uptake of N and P, which are most limiting in the soil with fewer forces from soil resources. In addition, there was still more P accumulated than N due to the high N for ERM with high‐P supply. Low N in HCs corresponded with a lower colonization rate in roots but with high hyphae density in HCs; this result suggest that N and P availability might change the ratio of extraradical to intraradical hyphae length.     

冬油菜与杂草对长期不同施肥的差异性适应

研究长期水旱轮作条件下,施肥模式对田间冬油菜、杂草、土壤养分及冬油菜–杂草空间分布的影响,为长江流域冬油菜轻简化生产提供依据。2011年在湖北省粮油主产区——江汉平原布置水稻–冬油菜轮作肥效定位试验。2016年冬油菜收获期,调查农田杂草群落多样性和分析油菜–杂草–土壤养分三者状况。结果表明,长期土壤养分亏缺会显著影响油菜–杂草的生长和空间分布、形成不同的杂草优势群落。平衡施肥(NPK处理)有助于增加冬油菜根茎粗、有效分枝数、角果数和最终产量,降低杂草总生物量,提高杂草群落多样性Shannon-Wiener指数。与NPK处理相比,–N、–P和–K均会不同程度地降低籽粒、茎秆、角壳和杂草的养分吸收量,尤以–P处理降幅最为明显。与土壤养分含量的初始值相比,长期不施用化肥会引起土壤氮磷钾有效含量下降,尤其对于土壤钾,现有平衡施肥措施不足以弥补农田钾素亏缺,应重视秸秆还田的补钾效果。总之,长期科学的农田养分管理与调控措施,不仅可以降低杂草对冬油菜的危害、增强生物多样性和形成良性的油菜-杂草竞争环境,也有利于减少化学药剂使用、推动冬油菜的轻简化生产。     

小黑杨APETALA1基因的克隆与花芽发育中的表达模式

APETALA1(AP1)基因既是花分生组织特征基因又是花器官形成发育基因,在花发育过程中起着关键作用。根据Genbank已知的AP1同源基因序列设计合成一对简并引物,以小黑杨(Popu-lus simonii×P.nigra)cDNA为模板,采用PCR方法分离克隆得到一个AP1同源基因全长,推测是小黑杨AP1基因,命名为XxAP1(GenBank accessionNo.XM₀02316040.1)。XxAP1编码249个氨基酸,开放阅读框长度747bp,蛋白质分子量28.67kD,等电点9.45。同源性分析表明,它们的核苷酸序列与其他木本植物的AP1同源基因的一致性达70%以上,推测它们具有相似功能。试验分析表明,XxAP1第1至第60个氨基酸为MADS盒结构域,说明XxAP1蛋白以序列特异方式与DNA结合,具有转录调节因子功能,第75至174个为K盒结构域,它负责蛋白质与蛋白质间的相互作用,其大多数氨基酸具有亲水性,使XxAP1蛋白具有较好的水溶性,促进蛋白的流动性有利于基因的转录。采用半定量RT-PCR技术检测XxAP1在雄花芽发育过程中的表达模式,结果显...     

不同种植密度对高粱生长、产量及养分吸收的影响

为了明确密度与高粱[Sorghum bicolor (L.) Moench]农艺和经济性状以及养分吸收的关系,以‘晋杂23号’高粱为试验作物,采用大田试验方法,试验设4.5万株/hm2、7.5万株/hm2、10.5万株/hm2和13.5万株/hm2 4个种植密度,研究了不同种植密度对高粱生长、产量、产量构成因素以及养分吸收的影响。结果表明,随着密度增加,高粱株高显著增高,茎粗显著变细,单株叶面积和单株干重显著下降。在4.5万株/hm2~7.5万株/hm2范围内,籽粒产量随着密度增加呈显著性增加。密度在4.5万株/hm2~10.5万株/hm2时,单位面积穗数随密度增加呈显著性增加。随着密度增加,穗粒数显著性增加,而千粒重影响不显著。除磷以外,密度对高粱氮和钾吸收总量均无显著影响。密度对籽粒氮吸收量没有显著影响;在10.5万株/hm2~13.5万株/hm2范围内,磷和钾吸收量明显下降。与氮和磷不同,钾主要分配在秸秆中,只有少量钾转运到籽粒中。相关分析表明,种植密度与株高、生物产量、籽粒产量和单位面积穗数呈显著性正相关,而与茎粗、单株叶面积、单株干重、经济系数、穗粒数和千粒重呈显著性负相关。本研究表明,种植密度与高粱主要农艺和经济性状以及养分吸收息息相关,在高粱高产高效栽培中起着非常重要的作用。     

滴灌水肥一体化下施氮量对小麦氮素吸收及土壤硝态氮含量的影响

为探明华北地区山前平原水肥一体化条件下小麦合理的氮肥运筹。于2013-2015年2个小麦生长季,设置4个滴灌施氮量(N0-不施氮、N1-120 kg/hm~2、N2-240 kg/hm~2、N3-360 kg/hm~2)处理,研究滴灌水肥一体化下施氮量对小麦氮素吸收积累和土壤硝态氮含量的影响。结果表明:施氮量N1、N2和N3处理的小麦干质量及产量较处理N0显著增加,N1、N2和N3处理间无显著差异;施氮量对小麦茎秆的氮含量影响较大,但对籽粒氮含量的影响差异不显著;处理N3的小麦总吸氮量分别显著高于处理N0、N1和N2,但处理N1和N2之间无显著差异;氮肥收获指数以N2处理最高,氮肥当季回收利用率、氮肥农学效率、氮肥生产效率和氮肥利用效率均表现出随施氮量增加而降低的趋势;施氮量超过240 kg/hm~2,土壤硝态氮含量增加,且随种植年限的延长更加明显。采用一元二次方程拟合,获得小麦最高产量的施氮量为238.46~250.78 kg/hm~2,经济施氮量为174.28~207.18 kg/hm~2。综合考虑经济效益和生态效益,该条件下小麦滴灌经济施氮量以174~207 kg/hm~2为宜。