甘蓝型油菜两种纯合中芥酸基因型的分离和鉴定

本研究从中国高芥酸甘蓝型油菜品种华油8号与加拿大无芥酸品种Altex杂交的后代中,分离和鉴定出了两种不同的纯合中芥酸基因型,它们在一个基因座位上具有纯合的有芥酸基因E.在另一座位上具有纯合的无芥酸基因c,其基因型组成可分别用E_AE_Ae_Ce_C和e_Ae_AE_CE_C表示。二者的芥酸含量均为27%左右,基本上等于双亲的平均值。这两种基因型的杂交后代和回交后代的分离结果表明,其遗传行为与无芥酸和高芥酸杂交后代的结果相同,这再次证实了芥酸的遗传假说,同时也证实了这两种基因型的遗传组成。文后讨论了这两种基因型的可能用途。     

大银鱼卵细胞粘丝扫描电镜观察

用扫描电镜观察了大银鱼的第Ⅲ时相，第Ⅳ时相，第Ⅴ时相卵母细胞和受精卵的卵细胞粘丝的外部形态特征和它们的粘性，并讨论了卵细胞粘丝的功能。     

Anthelmintic Actions of the Cyclic Depsipeptide PF1022A and its Electrophysiological Effects on Muscle Cells of Ascaris suum

The cyclic depsipeptide PF1022A, given orally to mice, showed very good anthelmintic activity against Heligmosomoides polygyrus and Heterakis spumosa at 50 mg kg⁻¹. In vitro, PF1022A was very active against Trichinella spiralis and had good activity against Nippostrongylus brasiliensis at 1 μg ml⁻¹. An 18-membered enniatin analogue, JES 1798, showed good activity only against N. brasiliensis at 10 μg ml⁻¹. The optical antipode of PF1022A had poor activity even at 100 μg ml⁻¹. The effects of PF1022A on the membrane potential and input conductance of somatic muscle of Ascaris suum were examined using a two-microelectrode current-clamp technique. PF1022A did not antagonize the effects of the selective nicotinic agonist levamisole. PF1022A and an analogue, JES 1798, but not the PF1022A antipode, produced a small time-dependent increase in input conductance associated with no potential change. The increase in input conductance did not occur in the Cl⁻-free bathing solution, suggesting that the increase in input conductance was mediated by Cl⁻ ions. The addition of high concentrations of Ca²⁺ to the preparation after the addition of PF1022A did not lead to production of Ca²⁺-activated Cl⁻ channels, suggesting that its mode of action was not that of a Ca²⁺ ionophore. The mechanism by which the cyclic depsipeptide might increase the Cl⁻ conductance is discussed.     

甘蓝夜蛾人工饲料的研究

文章报道了以甘蓝叶、白菜叶、灰菜叶、大豆粉、菜豆粉、玉米粉、麦麸为主要原料配制的5种人工饲料对甘蓝夜蛾的饲养效果。结果表明,以甘蓝叶和大豆粉为主要原料配制的配方—人工饲料对甘蓝夜蛾饲养效果最好,甘蓝夜蛾不仅能正常生长发育,而且在产卵量、蛹重、幼虫存活率方面均优于天然饲料,用该人工饲料连续饲养4代甘蓝夜蛾,未发现饲养后代生命力减退现象,表明该人工饲料可用来室内大量连续饲养甘蓝夜蛾。     

南瓜疫病菌(Phytophthora capsici)毒素对寄主叶片超微结构的影响

文章对南瓜疫病菌(Phytophothora capsici)进行了毒素提取,并用该毒素处理南瓜幼苗,然后观察南瓜叶片超微结构变化。Phytophothora capsici毒素处理后,随着其浓度的增加和处理时间的延长,细胞质壁分离严重,质膜断裂;叶绿体膨胀严重,膜消失,基粒片层紊乱;线粒体膜、脊被破坏;核膜不均一。     

Study on Soil Improvement Measure of Plant Landscape Construction in Saline and Alkaline Area in Tianjin

Tianjin is located in North China, which is semi- arid climate, next to east of Bohai, but being influ- enced little by internal bay. The urban district and strand area locates in a crossing area between the al- luvial plain of Hai River and the land form…     

番茄斑枯病研究概述

文章对番茄斑枯病的研究动态和进展进行了综述,包括番茄斑枯病的病症与识别、生物学信息、流行规律、防治方法、番茄苗期抗性鉴定方法及寄主抗性遗传规律等内容。     

由地铁隧道工程引起地表横向沉降的预测分析

应用Peck法、Logan-Poulous法、随机介质理论等方法对由地铁隧道工程引起的地表横向沉降进行预测,并对上述方法的实质加以研究。用STS沉降预测系统对实测数据进行分析和计算,并通过实测曲线和预测曲线的比对分析,验证上述方法的预测精确性。     

大豆对SMV株系SC-11的抗性遗传及抗病基因的等位性研究

利用对我国北方大豆产区流行株系SC-11表现抗病的材料科丰1号、邳县茶豆、大白麻、齐黄22、诱变30、PI96983、Kwanggyo和感病材料南农1138-2、南农18-6配制的抗感和抗抗杂交组合,研究了对SC-11的抗性遗传以及不同材料的抗病基因间的等位性关系.结果表明,科丰1号、邳县茶豆、大白麻、PI96983、Kwanggyo、齐黄22、诱变30与感病品种杂交的F1表现抗病,F2呈3抗:1感分离比例,F2:3家系呈1抗:2分离:1感病的分离比率,证明它们对SC-11的抗性由单显性基因控制.PI96983×Kwanggyo和齐黄22×诱变30杂交后的F2群体未发现抗感分离,表明PI96983与Kwanggyo以及齐黄22与诱变30对SC-11的抗性基因是等位或紧密连锁的.大白麻与科丰1号、邳县茶豆、诱变30,科丰1号与Kwanggyo,PI96983与邳县茶豆杂交的F2呈15抗:1感的比例分离,初步表明大白麻与科丰1号、邳县茶豆、诱变30,科丰1号与Kwanggyo,PI96983与邳县茶豆所携带的抗SC-11的基因是不等位的,而且2个抗性基因独立遗传.     

皮南牛培育及新品种审定工作进展

1986年以来,以南阳牛为母本,引进意大利皮埃蒙特牛为父本,通过杂交创新、横交固定、世代选育培育皮南牛(外血75%)。采用常规育种与全基因组选择选育相结合,实现了精准选育,提高了选择效率。培育的皮南牛屠宰率、净肉率、眼肌面积大等优点,已基本具备新品种申报条件。