Synthesis and Anthelmintic Activity of 7-Hydroxy- 5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thiocarboxanilides

7-Hydroxy-5-oxo-5H-thieno[3,2-b]pyran-6-carboxanilides and -6-thio-carboxanilides represent a novel series of anthelmintic compounds, with broad-spectrum activity against important parasitic nematodes in sheep and dogs. In particular, an improved efficacy against Trichostrongylus colubriformis in sheep over the related 3-carbamoyl-4-hydroxycoumarins has been noted. New synthetic routes to the key intermediate, 7-hydroxythieno[3,2-b]pyran-5-one, have been developed.     

基于IPV6协议重构校园网的研究

为适应我国对第二代教育与科研网的建设,对互联网的IPV6协议进行了深入探讨,并在分析校园网的实际需求基础上提出了相应的解决方案,为校园网的重构提出了指导性的方法。     

贵州优良草种苇状羊茅规范化栽培施肥试验

2003~2004年采用二次通用旋转组合设计研究了氮、磷、钾不同施用水平对苇状羊茅鲜草产量的影响,获得优化回归模型为y=3426．86 623．07x1 220．75x3;主效应分析表明,增施氮、钾肥的增产效果十分明显,而增施磷肥的作用很小。经模拟寻优,获得年产鲜草4 864kg／667m2的施肥方案为每667m2施纯N 50kg、纯K2O 55kg。     

典型旱作农田土壤氧化亚氮排放的氨氧化微生物相对贡献

氨氧化过程对氧化亚氮（N2O）排放具有重要贡献。在不同土壤类型和农田管理下,氨氧化微生物类群对N2O排放的相对贡献组成规律还缺乏系统的研究。本研究选取典型农田耕层土壤（潮土、黑土、砖红壤）,以及有机肥改良的砖红壤剖面土壤,采用选择性抑制法（乙炔和辛炔）研究氨氧化细菌（AOB）、氨氧化古菌和全程硝化菌（AOA+comammox）以及异养硝化菌对土壤硝化潜势、净硝化速率及N2O排放的相对贡献。结果表明,在耕层土壤中,潮土、黑土和砖红壤的pH分别是8.0、6.7和5.7,硝化潜势分别是N 32.5、6.6和4.8 mg?kg-1?d-1,净硝化速率分别是N 7.1、3.0和0.5 mg?kg-1?d-1,7天N2O累积排放量分别是N 38.0、35.4和8.7 μg?kg-1。AOB主导耕层土壤的硝化潜势,对硝化潜势的贡献分别是82%、58%和100%。对于净硝化速率,在潮土和砖红壤中,AOB和AOA+comammox贡献相当（均在30%~40%）,而黑土中由AOB主导（72%）。AOB主导耕层土壤的N2O排放,对N2O排放的贡献分别是72%、92%和58%。在改良的砖红壤剖面土壤中,在0~20 cm、20~40 cm和40~60 cm,pH分别是7.0、5.5和4.9,硝化潜势分别是N 6.6、2.0和1.1 mg?kg-1?d-1,净硝化速率分别是N 4.1、0.9和0.2 mg?kg-1?d-1,N2O排放分别是N 16.3、6.5和2.8 mg?kg-1?d-1。随土壤由深层至表层,硝化潜势、净硝化速率及N2O排放显著提高。AOA+comammox主导表层硝化潜势及净硝化速率的提高（分别贡献63%和54%）,AOB主导N2O排放的增加（贡献54%）。本研究为制定与土壤氨氧化特性及土壤性质相匹配的N2O减排措施提供了新的科学依据。     

GM1 gangliosidosis in a Japanese domestic cat: a new variant identified in Hokkaido,Japan

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.     

ABA， H2O2与NO对木枣果实成熟生理的影响

在木枣白熟期分别用ABA、H2O2与NO供体硝普纳（SNP）喷洒果实,对其调控木枣果实成熟的生理基础进行了研究。结果显示用不同浓度的ABA,H2O2与SNP处理,枣果实中丙二醛（MDA）的含量明显升高,其中100 mg?L-1浓度时MDA含量为最高;Ca、Vc和β-胡萝卜素含量呈现先降低后升高再下降的趋势,其中75 mg?L-1浓度时Vc和β-胡萝卜素含量最高,50 mg?L-1时Ca含量最高;CAT和APX活性的变化呈现先升高后降低的趋势,75 mg?L-1时CAT和APX活性均最大;DNA含量的变化呈现先缓慢降低后升高再下降的趋势,ABA比H2O2、SNP处理对DNA含量影响更明显,100 mg?L-1时DNA含量最高。结论：ABA、H2O2与SNP对木枣果实发育成熟的影响效果较为明显,其生理效果在多方面表现基本一致,有加快木枣果实成熟衰老的作用。     

花桥板栗2号与邵阳它栗的比较研究

摘 要：本文以湖南省的花桥板栗2号和邵阳它栗为研究对象,从品种原产地的自然条件、品种特征特性等方面进行对比研究,认为花桥板栗2号和邵阳它栗具有一定的相似性但特性且各有优劣,宜扬长避短,实现板栗产业化生产。     

鸭亲本间遗传距离与杂交后代杂种优势关系的研究

运用随机扩增多态DNA方法,对樱桃谷鸭、麻鸭及其杂交一代和用F1作为母本再与父本番鸭属间杂交培育的三元杂交半番鸭(F2)基因组DNA进行了遗传分析。结果表明,RAPD可用于有效标记鸭品种之间遗传亲缘关系和杂种优势预测;在所使用的20种随机引物中,5个鸭种共产生760条带,其中多态性带568条,占总条带数的 74．7％,说明鸭群体具有较为丰富的遗传多样性;杂交一代多态性位点比例为67．9％,遗传相似度为0．822 7,三元杂交二代多态性位点比例为83．1％,遗传相似度为0．826 4。说明三元杂交子二代的杂种优势可以用杂种优势理论中的显形假说来解释。     

贮梨冷库生产金针菇时的CO2浓度变化及调控研究

针对闲置贮梨冷库进行金针菇生产易造成库内CO2过度积累而影响金针菇商品性能问题,采用观测和模拟试验,对贮梨冷库生产金针菇时的CO2浓度变化及排除方法进行了研究.结果表明:金针菇菌丝体和子实体发育过程中不断产生CO2,且所产生的CO2浓度随时间延长而增高;在冷库近底层增加新风口,与库门构成平抽气流的改建设计,能有效提高CO2的排除效果,机械通风并辅以化学吸收剂Ca(OH)2,可有效保障库内CO2浓度与金针菇子实体生长发育的不同阶段相适应.