Use efficiency of nitrogen from biodigested plant material by ryegrass

Apparent nitrogen‐use efficiency of the applied mineral N (NUE_min) in effluent from biodigested plant material (BE; C : N_org ratio 14:1; mineral N–to–total N ratio 0.5:1) and a nitrate‐based inorganic fertilizer (IF), both applied at two rates was investigated in a six‐month pot experiment with Italian ryegrass (Lolium multiflorum Lam.). Dry‐matter (DM) production was 7% lower and total amount of N in aboveground biomass was 8% lower in BE than in IF at 40 d after sowing (DAS), equal at 81 DAS, and higher in BE than in IF at 136 and 172 DAS. NUE_min calculated on the basis of accumulated N in aboveground biomass of ryegrass in fertilized treatments compared to a control without N application was significantly lower in BE than in IF up to the third cut (136 DAS). Total NUE_min, total N recovery, and amount of foliage DM were similar for both fertilizers at the end of the experiment. Root biomass, total DM produced including roots and stubble, the fraction of root N to total plant N, and soil mineral N at 172 DAS were higher for BE than for IF. Mineral N applied with biogas‐reactor effluent was almost as effective as the nitrate‐based mineral fertilizer used for comparison. Within the six‐month experimental period net N mineralization, estimated at 12% of organic N in effluent, was not substantial. Hence, the organic compounds in the effluent were relatively recalcitrant.     

福建省主要蔬菜氮磷钾营养特性及其施肥指标体系研究Ⅱ.主要蔬菜氮磷钾施肥效应及其土壤养分丰缺指标

采用田间试验研究福建主要蔬菜氮磷钾施肥效应,建立菜田速效氮磷钾丰缺指标.根据148个试验的空白区产量水平,将土壤肥力分为“高”、“中”和“低”3个等级.结果表明,土壤肥力对蔬菜产量贡献率为(50.9士12.8)％,随土壤肥力等级的降低而明显下降;空白区产量与平衡施肥产量之间存在显著的线性关系.肥效试验结果表明,叶菜类、...     

类人源猪H3N2亚型流感病毒HA,NA遗传进化分析

2007-2008年从吉林省某猪场采集疑似流感发病猪的鼻咽拭子,经病毒分离鉴定获得3株H3N2亚型流感病毒,分别命名为A/swine/Jilin/5/2007（Sw/Jilin/5/07）、A/swine/Jilin/19/2007（Sw/Jilin/19/07）、A/swine/Jilin/37/2008（Sw/Jilin/37/08）。HA进化树分析结果表明：3株H3N2亚型流感病毒属于近代人源病毒谱系;但是在NA进化树中,Sw/Jilin/37/08株与早期人源和近代人源关系密切,暗示它可能是早期人源和近代人源H3N2亚型猪流感病毒之间的过渡毒株,进一步证实猪在流感病毒种间传播过程中充当＂中间宿主＂作用。     

长期不同施肥处理对棕壤氮储量的影响

为揭示施肥对棕壤氮素状况的影响,利用29年长期肥料定位试验,研究了不同施肥处理条件下土壤全氮在0―60 cm土层的分布特征,并在此基础上计算0―60cm土层氮库的储量变化。结果表明,不同施肥处理土壤全氮含量、C/N比值均随土层深度增加而降低,其影响主要表现在表层;而对0―60cm 土层全氮储量有显著性影响（P0.05）。长期不同施肥处理后土壤全氮及其储量变化趋势是:高量有机肥区＞低量有机肥区＞化肥区＞无肥区＞试验前（1979年）,特别是高量有机肥和化肥配合施用效果最显著;单施化肥处理土壤全氮含量和储量虽有缓慢提高,但差异不显著。说明土壤氮素含量的提高与施肥措施密切相关,有机肥和化肥配合施用能提高土壤全氮含量和储量,是维持土壤肥力的最优施肥方式。     

Influence of water regime on growth,yield, and nitrogen uptake of rice

Abstract

Greenhouse and field experiments were conducted to study the effects of water regime on growth of rice. The greenhouse experiment investigated the effects of two water regimes‐continuous flooding and flooding with soil drying between crops for 2 to 3 weeks on the growth of rice during six cropping (for six week each) on seven soils varying widely in total N contents (0.07 to 0.35%). The results averaged for the 7 soils indicated that the drymatter production or N uptake of rice was not affected by the water regimes during the six croppings.

The field experiment conducted during the dry season for two consecutive years (1976 and 1977) on a near neutral clay soil studied the effects of three water regimes (continuous flooding alternate flooding and soil drying every 2 weeks, and continuous flooding with 2 weeks mid season soil drying after 6 weeks of transplanting) and three levels of fertilizer N (0, 100 and 200 kg N/ha as urea) on grain yield and N uptake of rice. The results confirmed the absence of any significant reduction in grain yield or N uptake as a result of any of the soil drying treatments during the growing season on the unfertilized plots carrying a rice crop. On the plots fertilized with 100 or 200 kg N/na, alternate flooding and drying resulted in a significant depression in both grain yield and N uptake. Soil analysis supported heavy losses of N in the fertilized plots that underwent alternate flooding and soil drying apparently by nitrification and denitrification reactions.

The results of this study suggest that alternate flooding and drying of soils in the presence of established rice plants itiay not cause a significant loss of nitrogen in unfertilized plots although in plots fertilized with high rates of N the losses may be large as indicated by the performance of rice crop.     

Mixed-species legume fallows affect faunal abundance and richness and N cycling compared to single species in maize-fallow rotations

Rotation of nitrogen-fixing woody legumes with maize has been widely promoted to reduce the loss of soil organic matter and decline in soil biological fertility in maize cropping systems in Africa. The objective of this study was to determine the effect of maize-fallow rotations with pure stands, two-species legume mixtures and mixed vegetation fallows on the richness and abundance of soil macrofauna and mineral nitrogen (N) dynamics. Pure stands of sesbania (Sesbania sesban), pigeon pea (Cajanus cajan), tephrosia (Tephrosia vogelii), 1:1 mixtures of sesbania + pigeon pea and sesbania + tephrosia, and a mixed vegetation fallow were compared with a continuously cropped monoculture maize receiving the recommended fertilizer rate, which was used as the control. The legume mixtures did not differ from the respective pure stands in leaf, litter and recycled biomass, soil Ca, Mg and K. Sesbania + pigeon pea mixtures consistently increased richness in soil macrofauna, and abundance of earthworms and millipedes compared with the maize monoculture (control). The nitrate-N, ammonium-N and total mineral N concentration of the till layer soil (upper 20 cm) of pure stands and mixed-species legume plots were comparable with the control plots. Sesbania + pigeon pea mixtures also gave higher maize grain yield compared with the pure stands of legume species and mixed vegetation fallows. It is concluded that maize-legume rotations increase soil macrofaunal richness and abundance compared with continuously cropped maize, and that further research is needed to better understand the interaction effect of macrofauna and mixtures of organic resources from legumes on soil microbial communities and nutrient fluxes in such agro-ecosystems.     

Litter decomposition and faunal activity in Mediterranean forest soils: effects of N content and the moss layer

Accumulation of soil carbon is mainly controlled by the balance between litter production and litter decomposition. Usually In Mediterranean forests there are contrasting conditions in the distribution of faunal activity and the moss layer that may have different effects on litter decomposition. Decomposition and faunal activity were studied by exposing litter of contrasting quality (Pinus halepensis Mill. and Quercus ilex L.) for 3.5 yr in three Mediterranean pine forests of the eastern Iberian Peninsula. The effects of mosses on decomposition and on faunal activity were studied by exposing P. halepensis litter either on moss patches or directly on the forest floor. Faecal pellet production was used as an indication of faunal activity. Water availability or soil characteristics seem to limit faunal activities in the drier sites. Faecal pellets were not found during the first stages of decomposition and in all sites they appeared when about a 30% of the initial litter had decomposed. Under wet conditions faecal pellet production was very high and a mass balance suggested that soil faunal activity may result in a net flow of organic matter from the lower organic horizons to the surface Oi horizon. Mosses slightly increased mass loss of pine litter probably as a consequence of high potentially mineralizable nitrogen in the Oa horizon of moss patches and also, perhaps, as a consequence of the higher moisture content measured in the Oi horizon needles sampled among the mosses. In contrast, moss patches reduced faunal activity. The effect of litter quality on mass loss was not always significant, suggesting an interaction between litter quality and site conditions. During the first stages of decomposition there was N immobilisation in P. halepensis litter (poorer in N) and N release from Q. ilex litter (richer in N). In conclusion, in these forests soil microclimate and/or N availability appear to be more important controlling litter decomposition than the distribution of faunal activity.     

光强、温度和N/P比对坛紫菜叶状体单性生殖发生的影响

为探究培养条件对坛紫菜叶状体生长和单性生殖发生的影响,研究了不同光强、温度和N/P比下坛紫菜叶状体的生长及单性生殖发生的特点。结果显示,当光强为10～40μmol/(m2·s)时,藻体生长缓慢、成熟晚、单性生殖发生晚;光强增至60～80μmol/(m2·s)时,藻体生长加快,成熟和单性生殖发生同时提前。温度为17～20°C时,藻体生长慢、成熟晚、单性生殖发生推迟;23°C时藻体生长最快;而29°C组的藻体虽然生长受到抑制,但藻体成熟和单性生殖发生得到促进。N/P比为16∶1处理组的叶状体比其他处理组(1∶1、4∶1、32∶1和64∶1)均生长变快,N/P比为64∶1时叶状体生长最慢,但易成熟、易发生单性生殖。因此,在高光强、高温和高N/P比下,藻体成熟和单性生殖发生均提早,说明这些因素对坛紫菜叶状体单性生殖的发生有重要影响。     

水库微囊藻毒素3种异构体的年变化过程研究

应用高效液相色谱,对华北地区某水库5个采样点水体中微囊藻毒素（MC-LR、RR和YR）3种异构体进行了为期1a的监测,研究3种异构体随时间的变化规律及相互关系。结果表明,水体中3种异构体（-LR、YR和RR）含量分别为0.941±1.338、0.365±0.647和（2.893±5.387）μg·L-1;3种异构体出现时间不一样,LR异构体出现时间最早,在4月下旬即可检测到,而RR和YR异构体则需到5月中旬才能检测到;3种异构体峰值出现时间也不一致,LR和YR异构体峰值出现在8—9月,而RR异构体出现时间较晚,到10—11月才达到全年最高值;LR和RR异构体的含量比较高,是主要毒素,5—11月,其比值由2.20降到0.04,这与水体中的N/P比值有关,它随N/P比值的减少而减少。     

应用~(15)N研究小麦花生两熟制氮肥分配方式对小麦、花生产量及N肥利用率的影响

在小麦花生两熟制条件下 ,全年氮肥两作 3次施用 (小麦基肥、追肥和花生基肥 )氮素利用率为 3 2 52 % ,一作两次施用 (小麦基肥和追肥 )氮素利用率为 3 7 0 1 % ,但前者土壤残留多 ,损失少 ,氮肥回收率为 69 2 4% ,较后者高 1 2 0 3个百分点。且前者有利于小麦、花生产量的形成 ,是小麦花生两熟制适宜的施肥方式。小麦当茬氮肥利用率为 1 6 80 %～ 3 5 63 %。夏直播花生当茬及前茬小麦基肥和追肥 (拔节期 )的氮肥利用率分别为 2 3 70 %、6 57%～ 7 70 %和 1 0 0 3 %～ 1 2 73 % ;施肥时间和施肥量对氮肥利用率影响较大