全文获取类型
收费全文 | 420篇 |
免费 | 16篇 |
国内免费 | 18篇 |
专业分类
林业 | 5篇 |
农学 | 2篇 |
8篇 | |
综合类 | 41篇 |
农作物 | 1篇 |
水产渔业 | 33篇 |
畜牧兽医 | 358篇 |
园艺 | 6篇 |
出版年
2023年 | 5篇 |
2022年 | 4篇 |
2021年 | 4篇 |
2020年 | 9篇 |
2019年 | 16篇 |
2018年 | 10篇 |
2017年 | 17篇 |
2016年 | 17篇 |
2015年 | 12篇 |
2014年 | 26篇 |
2013年 | 38篇 |
2012年 | 12篇 |
2011年 | 27篇 |
2010年 | 32篇 |
2009年 | 43篇 |
2008年 | 29篇 |
2007年 | 22篇 |
2006年 | 24篇 |
2005年 | 18篇 |
2004年 | 13篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 9篇 |
2000年 | 15篇 |
1999年 | 8篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 1篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1956年 | 1篇 |
排序方式: 共有454条查询结果,搜索用时 500 毫秒
41.
Bauer NB O'Neill E Sheahan BJ Cassidy J McAllister H 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(3):168-172
A 3-year-old Wirehaired Fox Terrier was presented to the University Veterinary Hospital, University College Dublin, for evaluation of chronic cough of 8-months duration. Bronchoscopy showed a severely dilated collapsed left principal bronchus filled with highly viscous white mucus. Cytologically, globular lipid-like material and round concentrically laminated crystalline structures were evident within the proteinaceous mucus. These findings resembled the calcospherites and granular caseous debris often observed in human tuberculous patients. A Ziehl-Neelsen-stained cytocentrifuged preparation of material obtained by bronchoalveolar lavage revealed a few acid-fast rods within macrophages, suggestive of tuberculosis. At necropsy, granulomas with caseous necrosis were present in the lung parenchyma, bronchial and mediastinal lymph nodes, liver, pancreas, and mesentery. Granulomas were adherent to both kidney capsules and to the diaphragm. Histologically, there was evidence of mild calcification within caseous granulomas, which was confirmed by von Kossa's stain. Using Ziehl-Neelsen stain, acid-fast rods were identified within granulomas; bacterial culture was positive for Mycobacterium bovis. The cytologic findings in this case have not been reported previously in dogs and demonstrate a possible correlation between tuberculosis and calcospherite-like bodies with caseous, globular material in bronchial mucus, similar to that described in human patients. 相似文献
42.
The effect of a water-soluble fraction (WSF) of a non-pathogenic strain of Mycobacterium phlei was studied in bovine subclinical mastitis (SCM) by measuring the myeloperoxidase and acid phosphatase enzyme levels in the milk leukocytes. Forty-five cows were divided into three equal groups. Group I, consisting of 15 healthy cows, served as the control, whereas groups II and III each contained 15 cows with subclinical mastitis on the basis of a positive reaction in the California mastitis test (CMT). The cows in group II received 100 microg of WSF in 5 ml sterile phosphate-buffered saline, pH 7.4 (PBS) once only, while those in group III received 5 ml sterile PBS daily for 7 days, both treatments being given by the intramammary route. Observations were made up to 30 days after treatment (AT). The CMT of the healthy milk was negative (0), whereas it ranged between 1 and 2 points in SCM. The somatic cell count (SCC) increased significantly (p < 0.05) on day 3, then fell steeply from day 7 up to day 30 AT in the cows in group II. A steady decrease in the total bacterial count (TBC) was observed in the group treated with WSF but the bacterial counts remained high in the groups treated with PBS. The mean acid phosphatase level was enhanced by 119% on day 3 AT in group II but only by 18.7% in the cows in group III. The mean myeloperoxidase level was enhanced by 100% in the cows in group II but only by 18% in those in group III on day 3 AT. This significant reduction in the bacterial load in infected cows caused by intramammary infusion of WSF may be due to activation of the microbicidal activity of the neutrophils, but this requires confirmation. 相似文献
43.
M.J.B.M. Weljtens P.G.H. Bijker J. Van der Plas H.A.P. Urlings M.H. Biesheuvel 《The Veterinary quarterly》2013,33(4):138-143
Summary Numerous epidemiological reports implicate foods of animal origin as vehicles of human campylobacteriosis. Pigs are probably an important reservoir of campylobacter and a potential source of human infection. In order to improve our knowledge of the epidemiology of campylobacter in pigs, the prevalence of campylobacter and its contamination of feed were monitored in eight pig farms. Faeces samples of pigs aged 11 and 22 weeks, and samples of rectal, ileal and gastric content at a slaughterhouse were collected for bacteriological examination. On 5 farms, subsequent groups of pigs housed in the same stalls was sampled, too. A selection of the campylobacter isolates was characterized with a genetic typing method (RFLP). More than 85% of the sampled porkers were shown to be intestinal carriers of campylobacter at all stages of fattening. Subsequent groups of pigs housed in the same stalls were all carriers, too. The level of campylobacters in the faeces tended to decrease as the pigs got older. There was no difference in the frequency and level of infection with campylobacter between porkers on different farms. The feeding system (wet feed versus dry pellets) did not seem to influence the prevalence of campylobacter although wet feed gave lower counts of Enterobacteriaceae in the faeces. RFLP‐typing showed a high diversity of campylobacter strains at each sampling on the farm. Similarities were seen between strains isolated during two subsequent samplings of the same group of pigs, but not between strains isolated on the same farm from subsequent groups of pigs housed in the same stall. This suggests that the piglets were already infected at a young age on the breeding farm. 相似文献
44.
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease or paratuberculosis, a chronic enteritis of ruminants. While Johne's disease is primarily expressed in the gastrointestinal tract, isolation of MAP from extra-intestinal tissues indicates that microbial dissemination via the haematogenous route may occur during the infection. This study examined the movement of peripheral blood mononuclear cells (PBMCs) infected with MAP and the dissemination of MAP following mycobacteraemia induced by IV inoculation over a time frame of 3 days. 相似文献
45.
MC Barron RP Pech J Whitford IJ Yockney GW de Lisle G Nugent 《New Zealand veterinary journal》2013,61(5):209-217
Abstract AIM: To determine, for a variety of environmental conditions, how long Mycobacterium bovis might remain viable inside the carcass of a brushtail possum (Trichosurus vulpecula) that died of bovine tuberculosis (Tb), and to measure the rate of contact between free-ranging possums and possum carcasses. METHODS: Lesions of M. bovis were simulated by inoculating excised spleens weighing 0.5–1 g with 0.2 mL liquid culture containing approximately 5 x 107 cfu M. bovis/mL. Simulated lesions were inserted into possum carcasses (n=48) at the peripheral lymph nodes. Carcasses were placed in the field at two sites (a tussock grassland and a podocarp-broadleaved forest site) and in two seasons (summer and winter) for up to 62 days. Survival rates of M. bovis were estimated by sampling the simulated lesions over time, and culturing the recovered lesion to determine if any viable M. bovis bacteria were present. The time taken for a free-ranging possum to first encounter a dead possum in its home range was estimated by live-trapping possums and fitting them with proximity loggers (n=13). A ‘contact’ was recorded if these possums came within 40–50 cm of proximity loggers fitted to possum carcasses. RESULTS: There were strong seasonal and site effects in the survival rate of M. bovis in possum carcasses. In the grassland habitat, no viable bacilli were cultured from any carcass after 3 days in summer, whereas in winter all samples were culture-positive for the first 20 days, and some were still positive after 27 days. The survival rates for forest habitat were intermediate between the results for grassland, and there were no culture-positive carcasses after 9 days in summer or 27 days in winter. In summer, infected carcasses (n=6) were first encountered by possums a mean 1.9 (range 0.4–6.7) days after placement. CONCLUSIONS: Possum carcasses were contacted by free-ranging possums within the period that viable M. bovis were shown to survive in a carcass. The risk of such infection is likely to be most significant in winter or in areas with microhabitats where the survival of M. bovis is high. However, the generally low survival rate of M. bovis in possum carcasses and the low frequency of possum-to-carcass contacts indicate this route of transmission alone could not maintain Tb in a possum population. 相似文献
46.
R.N. Chesterton 《New Zealand veterinary journal》2013,61(4):133-134
Extract Lameness is a significant problem in dairy herds in New Zealand. It affects production and causes cows to lose weight. If antibiotics are used, milk has to be withheld from the vat. Cows may go into extended anoestrus and some which fail to conceive have to be culled. Even in its mildest form lameness is an inconvenience to the normal management of the herd. 相似文献
47.
D.T. Beakenridge B.V.Sc. 《New Zealand veterinary journal》2013,61(6):166-167
Extract Mange in pigs caused by Sarcoptes scabiei var. suis is reported to be common in New Zealand, but, to the writer's knowledge, no investigation has been made of the number of infested farms in any area. 相似文献
48.
The effect of fertility control on the transmission of bovine tuberculosis in wild brushtail possums
AIM: To determine the effect of fertility control on the rate of transmission of bovine tuberculosis (Tb), caused by Mycobacterium bovis, in brushtail possums (Trichosurus vulpecula). METHODS: At two study sites with a history of Tb infection in the resident possum population, a sample of adult male and female possums (n=50), determined by palpation to be Tb-free, was surgically sterilised by gonadectomy. A sample of untreated Tb-free male and female possums (n=54) served as controls. Each study site was trapped every 2 months over a 3-year period, and the Tb status of the individuals in the trial assessed. At the conclusion of the trial, all remaining experimental individuals were killed, necropsied and examined for characteristic lesions typical of Tb. The rate of transmission of Tb infection was estimated using the incidence of tuberculous cases in the experimental animals and comparing it between sites, sex and sterilisation treatment. RESULTS: Sterilisation by gonadectomy resulted in a reduction in the rate of transmission of Tb in male possums by 53%, but a corresponding increase in sterilised females for reasons that are still unclear. However, this interaction was statistically weak (p=0.10). When the sexes were combined, there was no overall effect of sterilisation on the rate of transmission of Tb (p=0.43). Sterility treatment notwithstanding, there was a highly significant difference in the rate of transmission between the sexes (p=0.01), being almost one order of magnitude higher in untreated males compared with untreated females. CONCLUSIONS: Although lacking strong statistical support, these results suggest that fertility control that targets endocrine control of fertility may provide some additional benefits for disease control over that achieved by reductions in population density, by reducing the rate of transmission of M. bovis in male possums. However, additional studies are needed to confirm the validity of these results. The large difference in the rate of transmission of M. bovis in untreated males compared with untreated females suggests that transmission of Tb among males may be an important driver of the dynamics of Tb infection in possums. 相似文献
49.
Nagaraja R. Thirumalapura Willard Feria Eric Hue Corey Zellers Deepanker Tewari 《Journal of veterinary diagnostic investigation》2021,33(2):375
Johne’s disease (paratuberculosis) is an economically important disease of cattle worldwide. The disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), a fastidious gram-positive bacterium. PCR is increasingly used in diagnostic laboratories for the detection of MAP in fecal samples given the rapid test turnaround time and sensitivity and specificity comparable to fecal culture. However, efficient extraction of DNA for sensitive detection of MAP by PCR is affected by the complex lipid-rich cell wall of MAP and the presence of PCR inhibitors in feces. We evaluated a high-throughput nucleic acid extraction method (MagMAX core nucleic acid purification kit with mechanical lysis module) in conjunction with an hspX gene PCR for the detection of MAP from bovine fecal samples, which resulted in correct identification of all negative (13 of 13) and positive (35 of 35) proficiency test samples obtained from the National Veterinary Services Laboratories. In addition, all 6 negative and 50 of 51 positive diagnostic specimens tested were categorized correctly. 相似文献
50.
This case report describes tuberculosis (TB) due to infection with Mycobacterium bovis (M. bovis) in alpaca (Lama pacos) on a farm in Ireland. Two severely debilitated alpaca were presented to the University Veterinary Hospital, University College Dublin in November 2004. Bloods were taken, and haematology and biochemistry results were indicative of chronic infection. Radiological examination showed evidence of diffuse granulomatous pneumonia suggestive of tuberculosis. On necropsy there were granulomatous lesions present throughout many body organs including lung, liver, kidney, intestine as well on peritoneum and mesentery. Culture of acid-fast bacilli from lesions led to a diagnosis of tuberculosis due to M. bovis. The use of intradermal skin testing proved inefficient and unreliable for ante mortem diagnosis of tuberculosis in alpaca. Infection due to M. bovis should be considered among the differential diagnoses of debilitating diseases in alpaca, particularly those farmed in areas known to be traditional black spots for tuberculosis in cattle. 相似文献