A cadaver study comparing two approaches for performing maxillary nerve block in dogs

Objective To compare the success by inexperienced anaesthetists of using a modified infraorbital approach to the maxillary nerve with the traditional percutaneous approach. Study design Prospective, randomized, blinded controlled study. Animals Heads from 37 euthanized Beagle and Beagle cross dogs. Methods Four anaesthetists were recruited to perform two different approaches to block the maxillary nerve of the cadavers. The infraorbital (I) approach advanced an intravenous catheter along the infraorbital canal. Earlier measurements from scans of similar heads were used to assess suitable catheter size. The percutaneous (P) approach introduced a needle percutaneously just below the ventral border of the zygomatic arch. The side of the head where the technique was to be performed was randomized. A total volume of 0.5 mL methylene blue was injected in each approach. After completion of injections, head dissections were performed by an investigator unaware of the approach used and staining of the maxillary and pterygopalatine nerves was evaluated. Chi squared analysis examined the relationship between the methods (p < 0.05). Complications related to the techniques, such as intravascular/intraneural injection and location of the dye, were evaluated macroscopically. Results Maxillary nerve staining >6 mm was found in 64.9% (I) versus 21.6% (P) attempts; staining <6 mm was found in 27% (I) versus 21.6% (P); and no nerve staining 8.1% (I) versus 56.8% (M). Pterygopalatine nerve staining was found in 70% (I) versus 21% (P). The infraorbital approach demonstrated significantly higher maxillary and pterygopalatine nerve staining compared to the percutaneous approach (p = 0.001 for both nerves). No evidence of intravascular/intraneural injections was found. Conclusion and clinical relevance The infraorbital approach was more successful than the percutaneous approach when performed by inexperienced anaesthetists. No macroscopic complications were observed.     

马铃薯皮中黄酮类物质测定及含量

以马铃薯皮为试验材料,研究了黄酮类化合物提取方法,获得了提取马铃薯皮中黄酮类化合物的最佳条件,测量了马铃薯皮中体现抗氧化性的黄酮类化合物的含量。试验中,对马铃薯皮中黄酮类化合物进行了提取、分离,以1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)的清除率作为马铃薯皮中提取的黄酮类化合物含量及抗氧化活性的评价指标。通过单因素试验,确定提取溶剂、温度、时间、料液比等对提取结果的影响程度。并在单因素试验的基础上设计4因素3水平的正交试验,从而确定马铃薯皮中黄酮类化合物的最佳提取条件。结果表明,马铃薯皮中黄酮类抗氧化物的提取最佳条件为50℃的温度下用80%丙酮溶液按照料液比1:10的比例提取,提取时间为3 h。在此条件下测得DPPH的清除率为84.62%。在最佳提取条件下提取马铃薯皮中黄酮类化合物,并测得每克烘干后的马铃薯皮中含有约10.95 mg的黄酮类物质,与其它类果实果皮中具有相似的抗氧化活性的黄酮类物质的含量相比明显较高。     

间作植物根系对Pb斑块胁迫适应机制

为研究间作体系植物根系受Pb胁迫的形态学适应机制,利用根箱试验设计Pb斑块胁迫下玉米、小花南芥单作和玉米与小花南芥间作处理。结果表明:在Pb斑块(0、1000 mg·kg~(-1))处理根箱条件下,间作小花南芥根生物量的相对关系指数显著增加,小花南芥根趋向有Pb斑块生长,根系表面积显著增加,间作模式极显著影响小花南芥根系形态;间作玉米根生物量的相对关系指数显著降低,玉米根趋向无Pb斑块生长,Pb处理极显著影响玉米根系形态。径级在1.5 mmd3≤2.5 mm根表面积显著影响间作小花南芥与玉米对Pb的响应。研究表明,根间相互作用激发小花南芥根系趋向Pb斑块,玉米根系趋向无Pb斑块,揭示了超富集植物与作物间作体系促进两种植物根系趋向性适应的重要原因。     

基于大食物观的多元化食物供给体系构建研究

构建多元化食物供给体系能够稳定和扩大食物供给,满足人们多样化食物需求的要求,促进农业食物系统转型。本文运用大食物观的思路,通过对相关政策、文献和数据的系统梳理,分析我国在构建多元化食物供给体系上的主要做法,探讨其不足,提出未来构建多元化食物供给体系的对策建议。研究表明,我国在提高国内粮食产量、保障重要农产品的供给、参与农产品国际贸易、树立大农业观和大食物观方面取得了很大的成绩,但同时也存在农产品供给与消费需求不匹配、食物消费多元化不足、国内农产品生产区域布局不平衡、国外农产品进口过度集中、农产品加工多样化程度不高等制约多元化食物供给的障碍。为了更好地构建多元化食物供给体系,应以大食物观来统筹粮食和重要农产品生产,丰富优化食物生产和消费结构,拓宽扩大食物来源,合理布局农产品生产区域,拓宽农业食物系统全产业链,统筹利用好国内和国际两个市场两种资源。     

探讨林地生态保护的作用及途径

生态保护工程是国家的绿化工程建设的重点,也是森林保护的重点内容,随着当前的 国家绿化建设发展加快,国家的绿化工程不断推动建设,林地的保护工作受到的重视程度加深, 部门的负责人员需要通过一定的措施,正视林地的生态建设过程中面临的问题,通过不断的探 索,寻求有效的途径,为生态保护工作的顺利进行提供重要的途径内容。     

雄安新区上游农业种植结构及需水时空演变

本文利用作物模型模拟小麦、玉米灌溉需水量,结合蒸发皿法估算蔬菜、果树等其他作物需水量,回溯雄安新区上游1986-2015年农业种植结构及农业需水的时空演变趋势,摸清不同作物的需水量比例及时间变化,并推算了消除降水年际波动的1970-2015年农作物灌溉需水量,探讨单纯人类活动下的农业需水量变化趋势。结果表明,1986-2015年,研究区作物播种总面积总体呈上升趋势,耕地面积多年平均84.9万hm²,有效灌溉面积平均71.3万hm²,占总耕地面积的84%。其中小麦播种面积稍有下降,玉米、蔬菜播种面积显著增加,果树种植比例在山区增加、平原区减少。研究区多年平均灌溉需水量22.52×10⁸ m³,小麦、玉米、蔬菜、果树和其他作物分别占灌溉需水总量的58.6%、12.6%、5.8%、16.3%和6.7%,受播种面积增加影响,1970-2015年,蔬菜和果树需水显著上升。从空间上来看,灌溉需水总量在上游山区上升显著,而在平原区表现为下降;排除降水的年际波动后,研究区作物需水自1970年以来一直呈上升趋势,进入20世纪80年代中期,虽然整体上升减缓,但随农业播种总面积增加和蔬菜、水果需水增加影响,需水量整体呈缓慢上升趋势。因此,控制上游农业用水,种植低耗水作物、减少耗水作物的种植面积,是恢复雄安新区清水产流的关键。     

土地利用对水体营养物影响的研究进展

日益严重的水质污染威胁到人类健康与生存,水体富营养化是其中一大治理难题。流域土地利用深刻影响水体中的营养物,定量刻画两者关系,可有效指导土地利用优化以改善水体质量。在国内外研究的基础上,本文梳理了样地实验、统计分析、经验模型和机理模型等4类研究土地利用和水体营养物关系的方法,并分析了不同方法应用的优缺点。以往研究多侧重土地利用数量结构,对强度和空间分布的量化及其与水体营养物关系的模型构建等研究尚显不足,多从单一或部分信息进行松散研究,导致不同研究中土地利用对水体营养物的解释能力差异显著。本文着重综述土地利用类型、强度差异和空间分布等3个方面对水体营养物影响的研究进展,并阐述关于两者定量关系尺度效应的争议和不确定性。目前主要存在问题包括现有研究土地利用和水体营养物的关系手段不足、土地利用多组分信息和水体营养物关系规律不清和尺度效应不确定性等3方面。因此,本文以水体污染物的产生、迁移、转化等的生态水文过程为线索,提出土地利用多信息空间综合表达和量化研究框架作为核心突破口。应用该框架,未来围绕融合过程机理与经验统计的模型开发、富营养化过程关联的土地利用多信息综合量化和多尺度结构的构建等3个方面开展研究,旨在为丰富土地利用对水体营养物影响研究提供一定的参考价值。     

基于ASI法的滨海滩涂地水稻测土推荐磷、钾施用量的验证

运用土壤养分状况系统研究法(ASI)进行土壤测定和水稻肥料推荐,通过田间小区试验对滨海滩涂地水稻推荐磷、钾施用量进行验证。结果表明,在其它营养元素的施用量均在最佳推荐量条件下,ASI法测土推荐的磷(P2O5)、钾(K2O)最佳施用量(90 kg/hm2、105 kg/hm2)处理的水稻有效分蘖数、穗实粒数、千粒重及产量均是最佳的;且施磷能够显著增加水稻的有效分蘖数,施钾能够显著增加水稻穗粒数和千粒重,最终达到增产的目的。因此,运用ASI法对滨海滩涂地水稻进行测土推荐磷、钾肥的施用量是基本合理的。     

The effect of approach direction and pace on flight distance of beef breeding cows

The aim of this study is to determine the effect of approach direction and pace on flight distance of beef breeding cows. Five Japanese Black breeding cows were used as experimental cattle. Three approach directions were tested; that were the approach toward the head of the test cow from the front direction (FRONT), the approach toward to the foreleg of the test cow from the right or left (SIDE) and the approach toward to hind‐leg of the test cow diagonally from the right or left rear (BACK). Three levels of approach pace were also tested; that were the approach at a pace of 7 km/h, the approach at a pace of 4 km/h, and the approach at a pace of 1 km/h. The shortest distance between observer and cow's at the moment of the cow's withdrawal was defined as the flight distance. The flight distance in FRONT was significantly shorter than that in SIDE. The effect of the approach pace was significant. The faster an observer approached to cow, the greater the flight distance shown by the cow. The manner of approach to cattle markedly affects the flight distance. Therefore, the manner of approach to cattle must be standardized to measure flight distance for sharing results obtained by several researchers under various conditions.     

超高效液相色谱-串联四级杆质谱法测定水产品中硝基呋喃类代谢物的方法改进

以GB/T 20752-2006中的实验方法为基础,对超高效液相色谱-串联质谱法(UPLCMS/MS)检测水产品中呋喃它酮(AMOZ)、呋喃西林(SEM)、呋喃妥因(AHD)和呋喃唑酮(AOZ)4种硝基呋喃代谢物的样品前处理方法进行了改进。试样用0. 2 mol/L盐酸溶液溶解后,经2-硝基苯甲醛衍生化,加入0. 1 mol/L磷酸氢二钾,混匀后用1 mol/L氢氧化钠溶液调节pH约为7. 4。经乙酸乙酯直接振荡离心提取上清液,氮气吹干经超高效液相色谱串联质谱,采用正离子多反应监测(MRM)模式检测,同位素内标法定量。结果表明,四种代谢物回收率控制在90%～120%之间,RSD10%。实际样品分析结果显示,新的样品前处理方法简单、分析时间短、结果可靠,适合水产品中硝基呋喃代谢物残留的测定。