不同类群绞股蓝的形态学研究

收集全国 6个省、市的绞股蓝 [Gynostemma pentaphyllum( Thumb.) Makino]类群样品 1 2个 ,进行形态学和花粉粒比较研究。结果表明 :不同产地的绞股蓝类群 ,其叶的小叶数、小叶的形状及大小存在变异。其主要的花粉形态有二类 :一类体型较小 ,表面雕纹呈条纹状 ;另一类体型较大 ,表面雕纹呈拟网纹状。     

穿龙薯蓣的药用价值及搭配栽培植物的研究

本课题组针对吉林西部生态环境特点,进行了穿龙薯蓣驯化栽培并获成功,现经试验筛选又确定了穿龙薯蓣与蒙桑、沙枣的搭配栽培种植模式。为此本文介绍了穿龙薯蓣的药用价值及蒙桑、沙枣的综合开发利用价值,此项研究前景非常广阔。     

冬季自然降温对三种蓝莓抗寒生理指标的影响

\t\t\t\t\t目的\t\t\t\t\t为了研究桃树花芽越冬过程中细胞内含物变化与花芽休眠的关系。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t以‘中华寿桃’（Prunus persica L. var. densa Makino cv. Zhonghuashoutao）为试材,分析比较越冬过程中8个时期花芽休眠状态和细胞内含物变化。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t‘中华寿桃’花芽在内休眠期间SOD活性呈现逐渐上升,随着内休眠解除,活性降低;POD活性在内休眠阶段出现先升高后降低的变化,内休眠解除过程中先降低后升高的变化;内休眠维持和解除过程中,CAT活性逐渐降低,而Vc出现几次较大的波动;内休眠阶段H₂O₂含量和\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tPurpose\t\t\t\t\tOur aim was to study the relationship between the changes of cell inclusions and flower buds dormancy during the winter.\t\t\t\t\t\t\t\t\t\t\t\t\tMethod\t\t\t\t\tDormancy status and the changes of cell inclusions were analyzed and compared in flower buds of Prunus persica L. var. densa Makino cv. Zhonghuashoutao at eight different periods during the winter dormancy.\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tResults\t\t\t\t\tThe results indicated that SOD activity gradually increased during the period of endodormancy, and decreased with endodormancy release. POD activity increased at first and then decreased in the stage of endodormancy, and firstly descended and then increased in the progress of endodormancy release. CAT activity gradually decreased and Vc content appeared several large fluctuations in the process of endodormancy maintenance and release. The variation tendency of H₂O₂ content and \t\t\t\t\t\t\t\t\t\t\t\t\t相似文献   

Measurement of Some Physiological and Biochemical Indexes of the Antisense Fragment of CYP86MF Gene Transgenic Male Sterile Plantlets in Chinese Cabbage-Pak-Choi

In order to study the mechanism of the transgenic male sterility induced by the antisense fragment of gene CYP86MF, some physiological and biochemical indexes were compared between the transgenic plantlets of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) and their controls.Results showed that there was significant difference between content of the endogenous hormones in leaves and floral buds. GA3 contents in leaves of the transgenic plantlets of B. campestris L. ssp. chinensis Makino cv.Shanghai-qing and cv. Youqing were reduced by 31.72 and 46.88% respectively as compared with those of their controls, and GA3 contents in floral buds of them were reduced by 62.92 and 80.57 % respectively. In addition, ZT contents in leaves of cv. Shanghai-qing and cv. Youqing were reduced by 11.81 and 181.20%respectively as compared with those of their controls and those in floral buds were reduced by 105.94 and 128.75% respectively. But, ABA content in transgenic plantlets was significantly higher than that in nontransgenic plantlets. However, differences among respiratory rate and cyanide-resistant respiratory rate, the photosynthesic rate and the content of total flavones in floral buds were not significant. In this study, the results indicated that the gene CYP86MF might be one of the key functional genes to control fertility of Chinese cabbage.     

恩施山区绞股蓝资源及野转家规范化种植技术

绞股蓝是葫芦科食药两用植物。该文总结了恩施自治州野生绞股蓝植物资源和野转家规范化种植技术，以期为绞股蓝植物资源的开发利用提供参考。     

Physiological Roles of Betacyanin in a Halophyte,Suaeda japonica Makino

Abstract: Halophytes can be used as a crop on salinized agricultural land. Suaeda japonica Makino is an annual highly salt-tolerant plant, that inhabits salt marshes in the Ariake Sea, Japan. Accompanying growth, leaves of S. japonica change from green to red with accumulation of a red pigment, betacyanin. To elucidate the physiological roles of betacyanin in S. japonica, we tested the antioxidant capacity of purified betacyanin and leaf extracts containing different levels of betacyanin under oxidative-stress conditions. The assay with 1,1-diphenyl-2-picrylhydrazyl (DPPH) showed that the purified betacyanin had antioxidant activity. H₂O₂-induced protein oxidation of the leaf extracts was prevented by the addition of betacyanin. The antioxidant enzyme activity decreased in the red leaves, but the content of malondialdehyde (MDA), an oxidative stress marker in the red leaves was nearly the same as that in the green leaves. Betacyanin synthesis was induced under photoinhibition-inducible conditions of low temperature and high-intensity light. These results indicate that betacyanin serves as an antioxidant in S. japonica and that the leaf reddening of this species is a key adaptive strategy for coping with the harsh environmental conditions in salt marshes of the Ariake Sea.     

白菜β-1,3 - 葡聚糖酶基因cDNA的克隆及序列分析

 以白菜抗霜霉病品种‘雪克青’cDNA 为模板, 采用RT2PCR 技术, 获得了1032 bp 的β- 1,3- 葡聚糖酶基因的cDNA 序列。序列分析表明, 克隆的β- 1,3 - 葡聚糖酶基因cDNA 序列编码343 个氨基酸, 与芜菁bg1 基因具有98 %的同源性, 与拟南芥bg2 基因具有84%的同源性。在GenBank 中登录号为AY395720 。     

不结球白菜不同品种或品系对POL胞质雄性不育恢保能力的研究

１９９３ ～１９９７ 年通过对不结球白菜ＰＯＬ胞质雄性不育系与不同品种或品系测交Ｆ１ 及后代兄妹交、回交的世代遗传分析,研究其对雄性不育的恢保能力。试验结果表明,６４ 份不结球白菜品种或品系对该不育系雄性不育性的恢保能力不同,可分为完全保持系（４ 份） 、完全恢复系（２ 份） 、半恢复半保持系（７ 份） 、高保持系（１７ 份） 、高恢复系（１ 份） 和不稳定保持系（３３ 份）６ 种类型。半恢复半保持测交Ｆ１ 的兄妹交及回交可育株∶不育株呈１∶１ 分离,可育株自交呈３∶１ 或全恢复可育性。控制该胞质雄性不育的细胞核基因由１ 对主效基因和若干微效基因所组成,恢复可育基因为显性,该不育性对温度敏感。     

大百合中铜和锰的原子吸收光谱直接测定方法

[目的]建立简便、快速、精确的大百合中高含量铜、锰的测定方法。[方法]采用次灵敏线（铜327．4nm、锰280．1nm）确定大百合中高含量铜、锰的分析条件,建立大百合中高含量铜、锰的次灵敏线原子吸收光谱测定方法,并考察酸及酸含量、共存离子等对测定结果的影响。[结果]含量低于10％的盐酸、硝酸、王水、高氯酸、硫酸对5mg／L铜和锰的测定结果无影响;2000mg／L K＋、Na＋,1000mg／LFe3＋、Zn2＋、mg2＋、Ca2＋100mg／LAl3＋、Si4＋及50mg／LPb2＋、Cd2＋、Cr6＋、Ni2＋均不干扰铜、锰的测定;铜、锰的线性jia范围分别为0-25、0—12mg／L,相对标准偏差分别为0．42％和0．66％,加标回收率分别为96％～103％和97％-102％。[结论]该研究建立的大百合中铜、锰含量的测定方法简便、快速、实用性强。     

有性多倍化白菜四倍体雄性不育花药发育的解剖学研究

[目的]对有性多倍化雄性不育系花药的败育时期和方式进行研究,为雄性不育基础研究提供理论依据。[方法]以同源四倍体白菜为母本,以秋水仙素诱导筛选出含2n配子较高的二倍体为父本杂交获得四倍体不育系及其保持系为材料,采用常规石蜡切片法对其花药进行解剖学研究,观察其花药的败育时期。[结果]退化的雄蕊可分为4种类型,均败育于孢原细胞分化期,始终处在孢原细胞期,无绒毡层与花粉母细胞的分化,不形成药室,属孢子体败育型。[结论]有性多倍化白菜四倍体雄性不育的4种类型都是孢子体败育型。