JZ_1型多功能电子时控巢础埋线机

本机采用电子时控电路在巢框线上直通8A 左右电流,使框线埋入巢础中。每框埋线约需2秒钟,埋1万框线耗电0.44度,快速、省电,且质量优越。同时还可用作废脾快速卸框和提供2～13V 直流电源蓄电池充电等.整机体积700×380×90mm,造价低,操作简便。     

BA-1对猪血发酵的研究

以BA-1为发酵菌种,鲜猪血为原料,采用固体、通风发酵法生产发酵血粉。结果表明,产品含鱼体必需的10种氨基酸,蛋白质含量为36.77％,水溶性物质总量和水溶性蛋白质含量分别比发酵前提高48.3％和170.15％,游离氨基酸总量为普通血粉的14.06倍。其中,必需氨基酸为11.52倍。鲤对发酵血粉的消化率为86.32％。表明BA-1作为血粉发酵菌种,效果显著。     

甘蓝两种SRK短截蛋白的体外表达及其与THL1作用检测

 为探明S–位点受体激酶（SRK）上与类硫氧还蛋白1（THL1）作用的氨基酸区域以及两者间作用方式,依据SRK_E1上功能域分布构建了两种SRK_E1短截体原核表达质粒pGEX-SRK_E1A和pGEX-SRK_E1B,分别在大肠杆菌BL21中获得了可溶性表达。通过体外孵育检测蛋白质相互作用的方法对SRK_E1A、SRK_E1B与THL1相互作用进行了检测,结果表明SRK_E1A和SRK_E1B均可与THL1结合,明确了THL1与SRK相互作用并不依赖于SRK激酶活性。两类SRK等位序列间比对结果表明Cys在两类SRK间的保守性存在明显差异,推测两类SRK材料亲和性的差异可能与Cys保守性不同有关。     

Hybridization among Epimedium (Berberidaceae) species native to China

The hybridization of fourteen plant populations belonging to seven Epimedium species native to China was studied by self-cross, infra-population cross, inter-population cross within species, and interspecific cross. Self-pollination studies on nine populations indicated high incompatibility; capsule-set rates were higher than zero in only three (4.61–6.76%). Interspecific cross-pollinations demonstrated high crossabilities in most cross combination (15.38–92.44% capsule-set rate), and the F₁ seeds possessed high germination rates (>20%). The F₁ hybrids of three interspecific cross combinations were raised to maturity. The morphology, karyomorphology of somatic cells, and pollen mother cell (PMC) meiosis of these F₁ plants revealed that they were all highly fertile (>76.10% in pollen viability), that there were few structural differences in chromosomes among species, and that most PMCs had 6 bivalents at MI. Abnormal chromosomal behaviors occurred in a minority, including chromosome bridges, unequal segregation of chromosome number, lagging chromosomes, and micronuclei. A series of experimental crosses provided strong evidence for an outbreeding system and a weak internal barrier to hybridizations in the taxa studied.     

Protective effect of recombinant SCR15-18 domain of human complement receptor type 1 on intestinal ischemia and reperfusion injury

AIM: To investigate the protective effect of recombinant SCR15-18 domain of human complement receptor type 1 (CR1-SCR-15-18) on intestinal ischemia and reperfusion in a rat model. METHODS: Sprague-Dawley rats were randomly divided into 3 groups: sham operation(SO) group, ischemia and reperfusion (I/R) group and CR1-SCR15-18 treatment group. The superior mesenteric artery of the rats was clamped for 30 min followed by 60 min of reperfusion. PBS alone or CR1-SCR15-18 protein (30 mg/kg) in PBS was intravenously administered 5 min before reperfusion. Intestinal vascular permeability, myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. The histopathological changes of intestinal mucosa were examined by HE staining and complement 3 was detected by immunohistochemical analysis. RESULTS: Compared with SO group, the vascular permeability, the activity of MPO and the content of MDA in I/R group were significantly increased, and the activity of SOD was decreased. HE staining demonstrated that I/R induced severe intestinal histological damages and the increased amount of complement 3 and its derivates were deposited in the necrosis area. Compared with I/R group, the vascular permeability, the activity of MPO and the content of MDA were decreased and the activity of SOD was significantly increased in CR1-SCR15-18 treatment group. CR1-SCR15-18 also significantly attenuated intestinal histological injury, and reduced the deposition of complement 3 and its derivates in the necrosis zone. CONCLUSION: sCR1-SCR15-18 protein exerts a protective effect against intestinal I/R injury in rats, possibly by inhibiting the activation of complement.     

Effects of Rho kinase on p-Smad1 nuclear translocation in rat pulmonary artery smooth muscle cells

AIM: To explore the mechanism of bone morphogenetic protein (BMP) and Rho kinase signal pathways on the proliferation of pulmonary artery smooth muscle cells. METHODS: Pulmonary smooth muscle cells were isolated from the rat distal pulmonary artery and cultured. BMP and Rho kinase pathways were activated by BMP-2 and platelet-derived growth factor BB(PDGF-BB),respectively. Rho kinase inhibitor Y-27632 and MEK inhibitor U0126 were also used. Immunofluorescent staining was applied to observe p-Smad1 distribution across the nucleus, and the cells with positive p-Smad1 nuclear accumulation were counted and the nuclear translocation rate was calculated. The total p-Smad1 and its distribution across the nucleus were quantitatively determined by Western blotting. The cell proliferation was analyzed by CCK-8 assay. RESULTS: Exposure to BMP-2 significantly increased both the total amount of p-Smad1 and its nuclear accumulation in pulmonary smooth muscle cells. Pretreatment with PDGF-BB significantly decreased the nuclear accumulation of p-Smad1 induced by BMP-2 without decrease of total p-Smad1. However, pretreatment with Y-27632 or U0126 reversed the inhibitory effect of PDGF-BB on p-Smad1 nuclear accumulation. BMP-2 significantly inhibited the cell proliferation, but PDGF-BB blocked the effect of BMP-2 and significantly increased the cell proliferation. After pretreated with Y-27632 or U0126, the PDGF-BB-activated cell proliferation was suppressed.CONCLUSION: PDGF-BB-activated Rho kinase inhibits BMP-2-induced p-Smad1 nuclear translocation via MEK/ERK1/2, and increases pulmonary artery smooth muscle cell proliferation.     

茄子新品种哈农杂茄1号的选育

哈农杂茄1号是以T142为母本,以T72为父本杂交配制而成的茄子一代杂种。早熟,植株长势强,叶绿色,茎紫色;果实长棒形,长25～28 cm,横径4.5～5.0 cm,果皮黑亮,绿萼,色泽好,果肉白色细嫩,单株结果10～12个,单果质量130～140 g,生育期110～115 d（天）;高抗黄萎病;耐弱光,耐贮运;平均每667 m2产量5 000 kg以上,适合在各紫长茄产区种植。     

基于Hydrus-1D模型的冬小麦根系层水分渗漏分析

利用Hydrus-1D模型模拟了河北省石津灌区冬小麦水分渗漏情况。结果表明,研究区40％的灌溉降雨量和38％的总供水量流出了200cm边界层,通过设置12种方案得到初始饱和度、2次春灌水量是影响灌区渗漏的主要因素,而二水时间间隔和第一水灌水日期影响相对较小。通过设置426种方案,分别得到了不同初始饱和度时,2次春灌灌水...     

Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice

Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Akt1 KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery.