2型猪链球菌的血清学鉴定

本试验首次证实了２型猪链球菌在我国的存在，采用玻片凝集、琼脂扩散及毛细管沉淀等３项试验，对１９９０年以来广东某猪场断奶仔猪暴发流行的败血症、脑膜炎及关节炎病猪中分离的１５株链球菌进行了血清学鉴定。结果表明：１５株链球菌均为２型猪链球菌；用高压法提取抗原进行沉淀反应，其结果比酸热法提取抗原具有简便、敏感、特异性强的特点。     

家蚕第2隐性赤蚁的遗传学研究——Ⅱ.ch-2基因的连锁分析

Ch-2基因是继ch之后新发现的一种隐性赤蚁ch-2基因与pM(2)、Ze(3)、L(4)、Pe(5)、E~(EL)(6)、q(7)、st(8)、I(9)、w-2(10)、ms(12)、cf(13)、U(14)、Se(15)、cts(16)、B_m(17)、nb(19)、rb(21)、or(22)、sp(23)、Nd(25)、及Y_m等各标志基因都是独立遗传.Ch-2与mln杂交F_2代分离+ch-2+min:ch-2+min:ch-2mln:ch-2mln=523:284:231:0≈2:1:1:0;ch-2与elp杂交F_2代分离+ch-2+elf:ch-2+elp:+ch-2elp:ch-2elp=219:97:68:0≈2:1:1:0,充分说明ch-2与mln、elp是连锁遗传的,即ch-2基因位于第18连锁群.     

陆地棉核雄性不育材料在山东不同地区的育性转变及应用研究

对在山东的济南、平度、莱西、烟台种植的几种陆地棉（Ｇｏｓｓｙｐｉｕｍ　ｈｉｒｓｕｔｕｍＬ．）核雄性不育材料的育性表现进行了研究,结果表明：洞Ａ不育系的衍生材料ＭＢ、ＭＡ在不同气候条件下有育性的变化。在济南高度不育,可作为不育系。在其他几个地区表现不同程度的可育。其中ＭＢ在平度有相当好的育性,能正常结铃。而双隐性核不育材料５９Ａ、６２Ａ未能观察到育性变化。本文就核不育材料在棉花杂交制种中的应用前景及需深入研究的问题作了探讨。     

包心菜无菌苗子叶及叶片原生质体高频分裂和高频植株再生

以包心菜子叶和叶片原生质体为材料。经不同液体培养基(Bp1,Bp2,B1)浅层培养,再生细胞高频分裂并形成愈伤组织。愈伤组织经扩增后转移到分化培养基上诱导植株分化,从这两种原生质体中获得了再生植株。在原生质体培养过程中,原生质体及细胞团的褐化程度与培养基中的有机成分的多少有关。原生质体的分化频率与培养基中植物激素种类关系甚大。在植株分化时,谷氨酰胺和腺嘌呤对植株分化有很大促进作用。另外,原生质体的来源及原生质体培养基对原生质体植株分化能力均有不同的影响。     

THE CLIMATE CHANGE AND FORESTRY OF HEILONGJI ANG PROVINCE IN 1990''''S

INTRODUCTIONIntheworldoftoday,theglobalclimatechangeanditsinfluenceonecologyhavebe-comeaveryimportantproblem,towhichmanyscientists,governnentleadersandordinarypcoplepaycloseattentionI1-'].Inl979,theWorldClimateResearchProgram(WCP)waslaiddowninthefirstworldclimatemeeting.lnl99(),thesecondworldclimatemeetingwasconvcl1edinGencva,andalltl1eexpertsagreedthattheglobalwarmlngwillbeextremelyseriousdisasterthananynatUralcalamityever.Attl1eMectingof"WorldEnvironmentandDevelopment,"holdinBrazil…     

Dynamics ofRhizoctonia solani (black scurf) in successive potato crops

The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3.     

Seasonal pattern of shoot emergence and its endogenous control in horsenettle (Solanum carolinense L.)

Controlling established horsenettle plants is achieved by suppressing shoot emergence from root systems. The seasonal pattern of shoot emergence and its possible endogenous control in horsenettle ( Solanum carolinense L.) were investigated. The shoot emergence period in an undisturbed population was limited to a seven-week period from mid-April, and a little longer in tilled conditions. Detached roots showed very high shoot-sprouting ability under 15–30°C throughout the year. In shoot clipping experiments, new shoots sprouted only from the stem and not from the root when attached to shoots, whether above-ground or underground. On the contrary, new shoots sprouted from the roots when all parts of the shoots were clipped off. From these results, the limited shoot emergence period in horsenettle is thought to be initiated by temperatures necessary for sprouting and is ended by a growth correlation effect between early emerged and matured shoots.     

外源Ca2+ 和ABA 对高温下黄瓜花药中Ca2 + 、ABA及蛋白质合成的影响

 外源Ca^{2 +}能降低高温下黄瓜单核期花药的胞内Ca^{2 +}水平并提高花粉萌发率, 而ABA的作用与此相反。外源Ca^{2 +}和ABA处理能显著提高高温下黄瓜花药内源ABA的水平, 并产生新的蛋白质。以上结果表明, 高温下外源Ca^{2 +}可影响黄瓜花药内ABA信号系统, 继而可能改变基因表达, 并获得耐热性;外源ABA亦能影响黄瓜花药内Ca^{2 +}信号系统。     

甘肃荚蒾属(Viburnum L. )观赏植物资源的调查研究

 在对甘肃荚蒾属植物资源调查的基础上, 整理并描述了27个分类群的生境、分布以及观赏性状, 包括叶、花、果实等, 并结合该属植物的资源现状, 提出了可持续开发利用的建议。     

黄瓜全雌性基因连锁的AFLP和SCAR分子标记

 本研究以全雌品种‘戴多星’自交系和弱雌品种‘北京截头’自交系为双亲杂交获得F₁ ,然后得到F₂ 性型分离群体, 利用分离群体分组分析法(Bulked Segregant Analysis, BSA) 构建全雌和弱雌两个基因池, 筛选了64对AFLP选择性引物EcoR I-NN +Mse I-NNN组合, 发现EcoR I-TG +Mse I-CAC引物组合在全雌基因池中扩增出一条分子量为234 bp的特异带。经F₂ 代单株验证, 该特异条带能在全雌单株中稳定出现。以MAP MAKER (Version 310) 软件分析, 该标记与全雌性位点的连锁距离在617 cM。命名该连锁标记为TG/CAC₂₃₄。将该特异条带回收、克隆、测序, 设计特异SCAR引物, 再对F₂ 代单株基因组DNA进行扩增, 仅在全雌单株中扩增出1条分子量为166 bp 的特异带, 表明已成功地将与黄瓜全雌性连锁的AFLP标记转化为操作简便、表现稳定的SCAR标记, 该标记命名为SA₁₆₆。