酵母及藻类对褶皱臂尾轮虫培养效果的比较研究

采用种群累积培养法研究了两种酵母、两种藻类对褶皱臂尾轮虫的最适投喂密度。结果表明 ,这四种饵料对褶皱臂尾轮虫的最适投喂密度 (× 10 6cell·ml-1)分别是 :干酵母 ,15 ;鲜酵母 ,5 ;微绿球藻 ,4 5 ;三角褐指藻 ,2 0。同时 ,在最适密度下比较了这四种饵料对轮虫的培养效果。结果表明 ,四种饵料对轮虫的培养效果以微绿球藻为最优 ,其它依次为三角褐指藻 ,鲜酵母 ,干酵母。实验结果显示 ,褶皱臂尾轮虫批量培养及至工厂化培养的首选饵料是微绿球藻和三角褐指藻     

Analysis of flowering,stigmas yield and qualitative traits of saffron (Crocus sativus L.) as affected by environmental conditions

Saffron is well known for its use as a condiment spice, as a dye and traditional medicine. Saffron is experiencing an increasing interest mainly due to its peculiar and manifold properties of the metabolic pool of its stigmas, mainly crocetin esters and picrocrocin. This species is cultivated in environments with very different climatic conditions and with very different corm rates from place to place, passed down over the centuries. The aim of this study was therefore the evaluation of the influence of rainfall, temperature and corm density on flower phenology, stigmas yield and main compositional characteristics of two saffron corms provenience. Flowering beginning in saffron seems to be influenced by the combination of temperature and soil moisture, whilst its flowering calendar proves independent of corm provenance, environment and plant density. On the contrary, the studied factors exert a strong effect on both total stigmas yield and qualitative characteristics: colder environment resulted in a higher flower production, but lower quality of stigmas. Flower number was positively correlated with the stigmas yield, but negatively with its unitary weight. The content of crocetin esters and picrocrocin has been evaluated according to the spectrophotometric ISO normative, which ranked the samples into three qualitative decreasing categories (I–III). The spectrophotometric data showed positive correlation with the unitary stigmas weight and negative with stigmas production.     

厦门筼筜湖游泳生物资源状况

2007年11月和2008年5月,对筼筜湖的游泳生物开展了2个季度的调查.结果显示:调查出现的游泳生物种类有69种.其中鱼类44种,虾类7种,蟹类14种,头足类3种,口足类1种;渔获量的优势种为远海梭子蟹和尼罗罗非鱼,渔获尾数优势种则以硬头鲻和远海梭子蟹为主.湖内刺网平均单位面积渔获量1.0069g/m2·h,单位面积渔获尾数0.0192ind/m2·h.排水口年平均资源指数为1043.317g/网·h,资源密度为148.883ind/网·h.现存资源量为729.752kg,现存资源尾数为10.4168×104尾.调查结果表明,筼筜湖的游泳生物种类比2001年出现的60种和1992年的43种,分别增加15%和60.46%.表明筼筜湖的综合治理取得了明显的生态效果.     

Condition assessment of lamprey populations in the Yorkshire Ouse catchment,north‐east England,and the potential influence of physical migration barriers

• 1. River lamprey (Lampetra fluviatilis) and sea lamprey (Petromyzon marinus) are designated features of the River Derwent Special Area of Conservation (SAC) and the Humber Estuary (a possible SAC). This study determined the condition of lamprey populations in the Yorkshire Ouse catchment by assessing the species composition, distribution, abundance and size‐structure of larval (ammocoete) populations in five major tributary rivers (Derwent, Swale, Ure, Nidd and Wharfe).
• 2. According to EU condition assessment criteria, Lampetra populations (assumed to be mostly river lamprey) are at present in favourable condition, with site mean (±SE) densities ranging from 2.7 (±1.2) to 160.3 (±50.5) individuals m^?2 (all river means >2 individuals m^?2), and at least two size (≈age) classes present in optimal microhabitats. By contrast, no sea lamprey larvae were recorded, suggesting that populations of this species are in unfavourable condition.
• 3. Actions to protect and enhance nationally or internationally important stocks must be implemented from at least a catchment perspective, because many of the issues affecting such species are not localized. With respect to lampreys, particular attention should be given to protecting spawning and nursery habitats, improving water quality, reducing impingement at abstraction points, preventing exploitation at spawning grounds and increasing passage at potential physical obstructions.

Copyright © 2007 John Wiley & Sons, Ltd.     

Effects of density on growth and survival of juvenile Red Swamp Crayfish, Procambarus clarkii (Girard), reared under laboratory conditions

The aim of this study was to quantify the importance of population density on the growth and survival of juvenile red swamp crayfish, Procambarus clarkii (Girard), and to understand the effects of density on both the period between moults and the increment per moult at high densities without food limitation. A laboratory experiment was performed to determine growth and survival of juvenile crayfish at five densities (20, 40, 60, 80 and 100 crayfish m^?2). Density clearly affected crayfish growth. The impact of density on final weight was higher than on total length increase, growth rate and final length (FL) (by decreasing order of effect). When comparing minimum and maximum densities, we observed that FL suffered a 34% reduction. Number of moults, mean intermoult period (IP) and survival were not significantly affected by density. Our results indicated that the IP and the percentage of increment per moult are affected by the size of crayfish before moult and we provide equations for these relations. Our findings are relevant both for crayfish aquaculture management and for the management of wild or harvested rice‐field crayfish populations.     

Effects of cage netting colour and density on the skin pigmentation and stress response of Australian snapper Pagrus auratus (Bloch & Schneider, 1801)

The unnaturally dark pigmentation of cultured Australian snapper Pagrus auratus can be improved through dietary astaxanthin supplementation and by holding fish in tanks with a white background. The practical application of these laboratory‐based findings was examined with two experiments to establish if the advantages of transferring fish to light coloured tanks before harvest could be achieved on‐farm using white cages and to determine the effects of fish density on skin colour. For the first experiment, snapper (mean TL=29.7 cm) were transferred from a commercial snapper sea cage to black or white netted cages and fed diets supplemented with unesterified astaxanthin (supplied as Lucantin^® Pink, BASF) at 0 or 39 mg kg^?1 for 42 days. Skin colour was measured using the CIE (black–white), (green–red), (blue–yellow) colour scale. Snapper held in white netting cages became significantly lighter (higher ) than snapper held in black cages; however, values were not as high as previous laboratory‐based studies in which snapper were held in white plastic‐lined cages. Snapper fed astaxanthin displayed significantly greater and values, and total carotenoid concentrations after 42 days. In addition, total carotenoids were higher in fish from black than white cages. The second experiment was designed to investigate whether density reduced the improvements in skin colour achieved by holding fish in white coloured cages and whether cage colour affected stress. Snapper (mean weight=435 g) were acclimated to black cages and fed 39 mg kg^?1 astaxanthin for 44 days before transferring to black or white plastic‐lined cages at 14 (low), 29 (mid) or 45 (high) kg m^?3 for 7 days after which time skin colour, plasma cortisol and plasma glucose concentrations were measured. Skin lightness () was greater in snapper transferred to white plastic‐lined cages with the lightest coloured fish obtained from the lowest density after 7 days. Density had no effect on plasma cortisol or glucose levels after 7 days, although plasma cortisol was elevated in snapper from black cages. For improved skin colouration we recommend feeding unesterified astaxanthin at 39 mg kg^?1 for approximately 6 weeks and transferring snapper to white plastic‐lined cages or similar at low densities for short periods before harvest rather than producing fish in white netting sea cages subject to biofouling.     

Effects of stocking density on haematological parameters,growth and fin erosion of great sturgeon (Huso huso) juveniles

The influence of stocking density (1, 2, 4, 6 and 8 kg m^?2) was investigated on haematological parameters, growth and fin erosion of great sturgeon (Huso huso) juveniles for a period of 8 weeks. The mean weight of fish at the start of trial was 93.13±1.04 g. After 8 weeks of rearing, the mean weight was 362.4, 319.7, 267, 242.1 and 211.1 in densities 1–8 kg m^?2 respectively. The results of this study showed that growth parameters, including condition factor, weight, weight gain, feed conversion ratio, specific growth rate and body weight increase, had a statistically significant difference among treatments (P<0.05). A significant difference (P<0.05) was observed in haematocrit, but the other haematological parameters, including red blood cells, white blood cells (WBC), haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentration and differential WBC count, showed no significant effect with stocking density (P>0.05). At the end of the experiment, stocking density had no significant effect on plasma cortisol and glucose concentration. The fin length (dorsal, anal, ventral, pectoral and caudal) of fish was measured to calculate the fin index. According to this index, dorsal, anal, ventral and pectoral fins showed no significant difference among treatments (P>0.05), but the erosion of the caudal fin was significantly different between fish held on 6 and 8 kg m^?2 (P<0.05). These results showed that rearing density has a major effect on the growth indices of H. huso. Unlike many other fish, great sturgeon exhibited lower stress responses to high stocking density. This indicates that they are more tolerable to rearing conditions in high stocking densities. With respect to the various effects that density causes on growth, fin erosion and physiological and haematological parameters, better understanding of these phenomena considering different levels of density could have a beneficial impact on many rearing steps of this species.     

Nitrate leaching in a silage maize field under different irrigation and nitrogen fertilizer rates

Quantification of the interactive effects of nitrogen (N) and water on nitrate (NO₃) loss provides an important insight for more effective N and water management. The goal of this study was to evaluate the effect of different irrigation and nitrogen fertilizer levels on nitrate-nitrogen (NO₃-N) leaching in a silage maize field. The experiment included four irrigation levels (0.7, 0.85, 1.0, and 1.13 of soil moisture depletion, SMD) and three N fertilization levels (0, 142, and 189 kg N ha⁻¹), with three replications. Ceramic suction cups were used to extract soil solution at 30 and 60 cm soil depths for all 36 experimental plots. Soil NO₃-N content of 0-30 and 30-60-cm layers were evaluated at planting and harvest maturity. Total N uptake (NU) by the crop was also determined. Maximum NO₃-N leaching out of the 60-cm soil layer was 8.43 kg N ha⁻¹, for the 142 kg N ha⁻¹ and over irrigation (1.13 SMD) treatment. The minimum and maximum seasonal average NO₃ concentration at the 60 cm depth was 46 and 138 mg l⁻¹, respectively. Based on our findings, it is possible to control NO₃ leaching out of the root zone during the growing season with a proper combination of irrigation and fertilizer management.     

基于核K—均值聚类算法的植物叶部病害识

针对植物叶部病害图像的特点,首先对采集到的玉米病害彩色图像采用矢量中值滤波法去除噪声,然后提取玉米病叶彩色图像的纹理特征和颜色特征作为特征向量,利用Mercer核,把输入空间的样本映射到高维特征空间进行K-均值聚类以及植物病害识别.试验涉及的4种玉米病害识别正确率达82.5%,核K-均值聚类方法适合玉米叶部病害分类.