Effects of BCL6B on proliferation and migration of human colorectal carcinoma LoVo cells and its potential mechanism

AIM: To detect the endogenous expression of B-cell leukemia/lymphoma 6 member B (BCL6B) in FHC and LoVo cells, and to investigate the effects of BCL6B on proliferation and migration of LoVo cells for further exploring the underlying mechanism. METHODS: The endogenous expression of BCL6B in the FHC and LoVo cells was detected by RT-PCR and Western blot. The methods of MTT assay, colony formation assay, wound healing assay and Transwell chamber experiment were employed to examine the biological functions of BCL6B in the LoVo cells. The mRNA and protein levels of BCL6B, cyclin D1 and matrix metalloproteinase-9 (MMP-9) were determined by RT-PCR and Western blot, respectively. The level of phosphorylated protein kinase B (p-AKT) was detected by Western blot. RESULTS: BCL6B expression was notably repressed in the LoVo cells as compared with the FHC cells, which were significantly increased by transfection with pcDNA3.1-BCL6B. The abilities of proliferation and migration of the LoVo cells at 72 h were inhibited by 28.33%(P<0.01) and 36.11%(P<0.05) in BCL6B group. The mRNA levels of cyclin D1 and MMP-9 in the cells of BCL6B group were decreased by 39.90%(P<0.01) and 77.36% (P<0.05), and the protein levels of cyclin D1, MMP-9 and p-AKT were reduced by 44.00%(P<0.05), 47.06%(P<0.01) and 32.88% (P<0.05), respectively. CONCLUSION: BCL6B inhibits proliferation and migration of the LoVo cells, and the PI3K/AKT signaling pathway is involved in this process.     

Effects of andrographolide on invasion and apoptosis of ovarian cancer SKOV-3 cells

AIM:To investigate the effects of andrographolide on the invasion and apoptosis of ovarian cancer cell line SKOV-3,and to explore the possible mechanisms.METHODS:SKOV-3 cells were treated with different concentrations (0,5,10,20 or 40 μmol/L) of andrographolide for different time (12,24,36 or 48 h),and then the cell viability was determined by CCK-8 assay.The cell invasion ability was analyzed by Transwell assay and cell apoptosis was detected by TUNEL staining.The protein levels of p-PI3K,p-Akt and p-mTOR were examined by Western blot.RESULTS:The results of CCK-8 assay revealed that andrographolide inhibited the growth of SKOV-3 cells in a dose-and time-dependent manner.Treatment with andrographolide at 20 μmol/L for 36 h significantly decreased the invasion ability of SKOV-3 cells,while increased cell apoptosis.In addition,the protein levels of p-PI3K,p-Akt and p-mTOR were reduced after andrographolide treatment.CONCLUSION:Andrographolide inhibits the growth and invasion of ovarian cancer SKOV-3 cells by suppression of PI3K/Akt/mTOR signaling pathway.     

微喷灌水肥一体化下磷钾肥减量分期施用对小麦产量和养分利用的影响

为探究微喷灌水肥一体化条件下小麦氮磷钾肥的合理用量及施用方式,以小麦品种良星99为试验材料,在磷钾肥减量30%和常规施肥量条件下各设置3种施肥方式,以传统大水漫灌撒施肥料的方式及用量为对照(CK),研究磷钾肥减施及施用方式对产量及养分利用的影响。结果表明,常规施肥(CK)相比,微喷灌水肥一体化磷钾肥减施30%(氮肥70%底施+30%拔节期追施、钾肥全部底施、磷肥50%底施+50%拔节期追施)条件下产量提高11.33%,成熟期植株氮、磷、钾积累量分别提高21.86%、10.02%和10.23%。与传统栽培模式及微喷灌常量施肥相比,利用微喷灌水肥一体化技术生育期灌水3次(越冬水+拔节水+灌浆水),灌水量1 500 m³·hm^-2,磷钾肥减施30%条件下,磷肥50%底施+50%拔节期追施能够提高小麦产量、成熟期植株氮磷钾积累量、偏生产力及产投比。本研究结果为山西省南部小麦节水节肥稳产高效栽培技术提供了理论依据。     

低钾胁迫下不同耐低钾玉米品种(系)开花后根系生长和结构的变化

[目的]土壤缺钾常引起植株早衰,严重影响玉米产量.从玉米开花后根系形态、结构和活性等参数的变化,研究低钾胁迫下玉米早衰的机理.[方法]以耐低钾玉米自交系90-21-3和钾敏感玉米自交系D937为试材,进行池栽试验.设置天然低钾土壤(低钾,-K)和正常供钾(CK,+K)两个处理,分别于玉米开花后测定根系形态、结构和活性等...     

小麦新品种陕农33的遗传构成分析

为解析小麦新品种陕农33的遗传构成,利用小麦55K芯片检测到的53 063个SNP标记分析双亲陕农981和新麦18对陕农33的遗传贡献率。结果表明,陕农33与亲本陕农981和新麦18 SNP标记的一致性分别为52.72%和46.38%,陕农981对陕农33的贡献略大于新麦18;从染色体水平看,新麦18对陕农33的贡献率超过50%的染色体有2A、5A、7A、3B、4B、2D、3D、4D和6D,而陕农981在除此之外的12条染色体的遗传贡献率均大于50%;在遗传距离大于5 cM的染色体区段中,陕农33来源于陕农981和新麦18的染色体区段分别有18个和19个,其中,在6B染色体上来源于陕农981的染色体区段最多(4个),在7A染色体上来源于新麦18的区段最多(6个);陕农33有154个不同于双亲的特异位点,分布在21条染色体上,其中,在1B、2B、2D、4A、4D和6A染色体上,共发现11个与农艺和品质性状有关的QTL,3个来源于新麦18,8个来源于陕农981。     

干旱胁迫对木薯K+、Ca2+和ABA的影响

为了研究干旱胁迫木薯K+、Ca2+和ABA对干旱胁迫的响应及其变化规律,对2个品种的木薯SC124（高抗）和KU50（低抗）进行对照、驯化、非驯化旱害和驯化旱害等不同方式的干旱处理。研究发现2个木薯品种中,干旱胁迫对叶片K+和ABA的含量影响较大,而Ca2+含量变化较小;SC124在驯化和非驯化处理下的K+含量发生显著变化;ABA的含量在2个品种中的驯化干旱和非驯化干旱中变化显著,且变化趋势不同;干旱胁迫下,ABA、K+和Ca2+之间存在显著相关性。     

施钙与覆膜对缺钙红壤花生氮、磷、钾吸收利用的影响

为探明施钙与覆膜栽培对南方典型缺钙红壤（第四纪红土发育而成）花生氮、磷、钾吸收分配的影响,本试验以大籽品种湘花2008为材料,设置3个基施钙肥梯度（不施钙、每公顷施钙肥375kg、每公顷施钙肥750kg,分别标记为Ca0、Ca375、Ca750）和2种栽培方式（露地OF与覆膜栽培PF）,采用土柱栽培,测定花生植株氮、磷、钾素含量、积累与分配特性。结果表明：随施钙量增加,花生叶、茎秆、根、果针、果壳氮素积累和分配率显著降低,而籽仁的氮素积累量和分配率、植株总氮素积累量提高,覆膜栽培降低叶和果针氮素分配率。增施钙肥有利于花生籽仁磷素的吸收积累、提高其分配率,但显著降低叶、茎秆、根系、果针、果壳磷素分配率。覆膜栽培提高植株对磷素的吸收,而降低茎秆磷素分配率。增施钙肥与覆膜栽培促进花生籽仁中钾积累,提高分配率。植株总钙积累量与总氮素积累量的协同吸收关系（决定系数R2=0.7837）优于总钙积累量与钾、磷总积累量的协同关系（决定系数R2=0.659 1、0.201 9）。增施钙肥提高了氮、磷、钾的利用效率和收获指数（HI）。露地播种每公顷750kg钙肥处理每生产100kg荚果比对照（Ca0-OF）节省纯N 3.17~4.04kg、纯P 0.27~0.30kg、纯K 2.65~3.41kg。因此,花生生产中增施钙肥的同时,可适当减少氮磷钾肥的用量,利于花生高产高效栽培。     

K型不育系K18A的选育及应用

K18A是四川省农业科学院水稻高梁研究所育成的具有粳稻胞质的新质源不育系，具有败良彻底，异交习性优良，可恢性好，配合力高，抗性好和稻米品质优等特点，1999年7月通过四川省成果鉴定，所配组合表现优势强，产量高，熟期早，其中K优817，K优8725，K优8149已于2000年8月通过了四川省品种审定。     

不同成熟度烤烟叶细胞化学研究

采用酶解方法分离K326不同成熟度烟叶中部叶(第10叶)栅栏细胞和海绵细胞,运用气相色谱分析鉴定细胞中非挥发性有机酸,分光光度计法分析质体色素、糖类及烟碱.发现多元有机酸在海绵细胞中含量较高,超过栅栏细胞的2倍以上,高级脂肪酸、质体色素、糖类均是栅栏细胞中含量高,总量分别超过海绵细胞的2,1.2,1.2倍.而海绵细胞烟碱含量高于栅栏细胞的2倍以上;在烤烟成熟过程中,有机酸、叶绿素和类胡萝卜素的含量逐渐降低,过熟海绵细胞有机酸、叶绿素和类胡萝卜素含量分别仅为未熟的50%,16%,50%.过熟栅栏细胞有机酸、叶绿素和类胡萝卜素含量仅为未熟的50%,20%,55%.糖类在欠熟细胞内含量最高,过熟细胞内最低,欠熟海绵细胞为过熟的1.6倍,欠熟栅栏细胞为过熟的2.4倍.烟碱随着成熟度增加而逐渐积累,适熟细胞内含量最高,未熟细胞内最低.适熟海绵细胞为未熟的2.8倍,适熟栅栏细胞为未熟的5.4倍.     

土壤缺钾对棉花钾运转和分配的影响

在盆栽条件下应用86Rb 标记和原子吸收分光光度法,研究了常规棉中棉所12、中棉所35和转基因抗虫棉新棉99B、中棉所41四个品种的钾运转和分配情况.盛花期中棉所35上部叶的86Rb 主要运转到主茎,中部叶的86Rb 主要运转到产量器官.缺钾促进了棉花下部主茎叶和果枝叶中的钾向上部和中部转移,有利于提高钾在植株体内的再利用;而抗虫棉新棉99B和中棉所41下部主茎叶和果枝叶中钾的转运能力较差,可能是它们在生产上后期易早衰的原因之一.产量器官是棉花钾营养的第一大和最终库器官,而主茎则担负着储存和中转的功能.土壤缺钾时,棉花主茎叶和产量器官的钾运转和分配比例提高;另外,种子和纤维的钾浓度随供钾水平的变化在棉株各器官中最小.这些结果说明,钾缺乏时棉株一方面会尽可能维持叶片的功能,从而满足棉株对同化产物的需求;另一方面会利用有限的钾资源优先保证后代的繁衍.