Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.     

Effect of Liucha extract on tumor K562 cell growth

AIM:To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism.METHODS:The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope.RESULTS:Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared.CONCLUSION:The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells.     

NaCl胁迫下枸杞愈伤组织活性氧产生与质膜H+—ATPase活性的关系

以枸杞愈伤组织为材料,研究了盐胁迫对活性氧伤害和质膜H -ATPase活性的影响。结果表明,NaCl浓度为100mmol/L时超氧阴离子(O2-·)和过氧化氢(H2O2)含量上升,质膜H -ATPase活性先升后降;质膜相对透性与丙二醛(MDA)含量呈显著正相关;超氧阴离子(O2-·)和过氧化氢(H2O2)含量与质膜H -ATPase活性在重度胁迫下呈显著负相关,说明盐胁迫下活性氧积累可能是加速伤害质膜功能的主要原因之一。     

外源乙烯对草莓果实微粒体Ca2+- ATPase活性和膜脂过氧化的调节

 以乳白期‘春星’草莓(Fragaria ananassa Duch．‘Chunxing’)果实为试材，研究了经钙调素(CaM)拮抗剂三氟拉嗪(TFP)、氯丙嗪(CPZ)各100 µmol·L^-1 和钙通道阻塞剂异博定(Verapamil)100 µmol·L^-1 预处理后再用乙烯(50 µL·L^-1 )处理的草莓果实中微粒体“Ca²⁺-ATP酶活性、O₂ 产生速率和MDA含量的变化。结果表明，外源乙烯对微粒体膜O₂ 产生速率无显著影响，处理早期提高微粒体膜Ca²⁺- ATPase总活性，对MDA含量影响不大；后期加速微粒体膜Ca²⁺- ATPase总活性下降，但仍保持较高的MDA含量和线粒体膜Ca²⁺-ATPase活性。CPZ、TFP和Verapamil预处理降低了上述乙烯处理下Ca²⁺- ATPase活性和MDA含量，但对微粒体膜O 产生速率亦无显著影响，这说明细胞内Ca²⁺-和CaM 可能参与了乙烯诱导的膜Ca²⁺-ATP酶活性与膜脂过氧化水平的调节。     

(土娄)土区不同蔬菜对钾肥的肥效反应

在陕西省关中土娄土区布置线辣椒、甘蓝、白菜、芹菜钾肥大田试验,研究不同蔬菜对钾肥的肥效反应。结果表明,增施钾肥可使蔬菜硝态氮含量降低11.8%～116.0%,维生素C含量增加0.4～147.4mg/kg;产量增加9.3%～33.8%;明显促进营养生长和生殖生长;400cm深土壤剖面硝态氮累积量减少22.7～48.0kg/hm2,尤其在80～220cm剖面减少最为明显。     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

南果梨周年干物质与氮磷钾积累动态

【目的】明确南果梨干物质积累特征和氮磷钾养分周年动态积累规律,为南果梨优化施肥量和施肥时期提供依据。【方法】以12年生南果梨树为试材,采用田间采样和树体分解方法,分别于萌芽后10 d(萌芽期)、 30 d(花期)、 65 d(幼果膨大期)、 100 d(果实膨大或新稍停止生长期)、 130 d(果实着色前)、 155 d(果实采收期)、 185 d(采收后)、 210 d(落叶前)8个生育期,选干周和树高一致的3株树,将树体连根从土壤中挖出,分出果实、 叶片、 枝条、 主干、 主根、 侧根、 须根,各器官单独称重,并取200 g左右的鲜样按清水、 洗涤剂、 清水、 1%盐酸、 3次去离子水冲洗、 杀青、 烘干后,电磨粉碎过0.15 mm筛,测定样品中氮、 磷、 钾含量。【结果】1)南果梨周年生育期内,树体干物质当年净积累量为18.4 kg/plant,干物质累积速率出现两次累积高峰,分别是幼果膨大期(0.15 kg/d)和采收期(0.11 kg/d)。2)南果梨树体总氮周年积累量为154.0~301.0 g,新生器官为0~116.2 g,果实膨大期达到最高;多年生器官氮积累量为154.0~194.8 g,落叶前达到最高。3)南果梨树体总磷周年积累量为17.1~37.2 g,果实着色前最高。其中新生器官为13.7 g,果实采收期最高;多年生器官为17.1~24.9 g,果实转色期最高。4)南果梨树体总钾周年积累量为27.9~174 g。新生器官钾为97.3 g,采收期最高;多年生器官钾为27.6~76.6 g,落叶前最高。5)产量大约为17 t/hm2的12年生南果梨从萌芽到落叶前树体当年氮磷钾的单株净累积量分别为146.2、 20.1、 146.1 g,折合1000 kg果实经济产量需吸收氮(N)、 磷(P)、 钾(K)5.4、 0.7、 5.4 kg。【结论】南果梨周年干物质单株积累总量为41.4 kg,当年净积累量为19.7 kg。南果梨干物质积累主要集中在花期到果实膨大期和果实转色到落叶前,分别占47.3% 和47.5%。南果梨从萌芽到落叶前氮、 磷、 钾的单株净累积量分别为146.2、 20.1、 146.1 g,每1000 kg果实经济产量需吸收氮(N)、 磷(P)、 钾(K)5.4、 0.7、 5.4 kg。从开花到果实膨大期和从果实着色到采收后30天对氮吸收分别占总氮累积量的39.0%和49.0%,而磷、 钾的累积从萌芽到开花较快,到果实膨大期磷的累积达67.4%,钾的累积达65.1%,果实膨大期是干物质和氮磷钾积累的关键时期。     

农五师葡萄钾素动态变化及调节措施研究

通过叶分析研究了不同葡萄品种叶柄全钾5～9月含量动态变化规律.结果表明:葡萄叶柄钾含量在生育期内整体呈波浪式下降趋势,前期下降迅速,中期平缓,进入浆果成熟期后又趋于下降；强势品种在整个生长季节内对钾的吸收大于弱势品种；早熟品种叶柄内钾含量变化趋势比晚熟品种提前.根据新疆农五师葡萄园区土壤有效钾含量分布现状,建议生产中应根据葡萄钾元素动态变化特点,不同团场应结合当地土壤有效钾含量相应补充.     

固相萃取-高效液相色谱串联质谱法测定油菜薹中反式维生素K1含量

为准确测定反式维生素K1的含量，建立了基于固相萃取-高效液相色谱串联质谱的甘蓝型油菜（Brassica napus L.）菜薹反式维生素K1高灵敏检测技术。样品经正己烷提取、中性氧化铝柱净化后，用C30反相色谱柱，以甲醇 （含0.025%甲酸+2.5 mmol/L甲酸铵）为流动相，采用选择反应监测（selected reaction monitoring, SRM）模式进行定量分析，20 min内可实现顺反异构体色谱分离。方法学考察结果显示，反式维生素K1在范围内线性关系良好，相关系数为0.9985。该方法检出限为0.29 μg/kg，定量限为0.95 μg/kg。回收率为87.5%～117.6%，精密度（RSD）在0.72% ～9.59%之间。利用该方法对油菜薹和反式维生素K1含量高的3种蔬菜进行分析，发现油菜薹中反式维生素K1含量为340.08 μg/100g，高于小白菜（B. rapa spp. chinensis，260.93 μg/100g）、西兰花（B. oleracea var. Italic Planch， 167.65 μg/100g）和结球甘蓝（B. oleracea var. capitata，151.11 μg/100g）。本文建立的蔬菜中反式维生素K1准确定量分析方法操作简单、灵敏度高、结果准确。同时比较发现油菜薹是一种富含维生素K1的蔬菜。      

Assessing the effectiveness of manure export plus intensive silage cropping for lowering the Olsen‐P status of P‐enriched grassland

A substantial proportion of farmed grassland soils in Northern Ireland (NI) are overly enriched with P and pose a risk to water quality. To address this problem, manure could be exported rather than recycled to P‐enriched land and the latter intensively cropped with grass silage to deplete soil P. To assess the efficacy of such a strategy, a P‐ and K‐enriched grassland site was intensively cropped over a 6‐yr period with fertilizer N alone supplied to support silage growth. By year 6, soil P had declined from index 5 to index 3, and it was estimated that two more years of this management may bring it into the target index 2 range. Soil K, however, declined rapidly from index 4 to index 1 in just 4 yr, with the result that grass production became limited by K deficiency. It was concluded that nonrecycling of manure to P‐enriched grassland under silage management is probably the most effective strategy for lowering soil P status, but care must be taken to prevent K deficiency occurring.