4种药剂除治红叶石楠容器苗杂草药效试验

用24%乙氧氟草醚EC、10.8%高效氟吡甲禾灵EC、24%乙氧氟草醚EC+10.8%高效氟吡甲禾灵EC、33%二甲戊乐灵EC 4种药剂对红叶石楠容器苗进行杂草除治试验。结果表明:用24%乙氧氟草醚EC20 mL/667m2进行杂草防治,药后30d株防效为65.6%、鲜重防效为80.8%,均明显好于其它3种药剂的除草效果,且对苗木较为安全,基本上能达到除治杂草的目的。     

Expression analysis of the type I keratin protein keratin 33A in goat coat hair

The coat of a goat, like that of many mammalian species, consists of an outer coat of coarse hairs and an under coat of fine, downy hairs. The coarse guard hairs are produced by primary follicles and the finer cashmere hairs by secondary follicles. We previously reported that hair keratins are components of cashmere hair, and proteomic analysis revealed that their expression is elevated in winter coat hair. To determine detailed characterization, we have cloned keratin 33A gene, a major highly expressed keratin in winter, and then analyzed the expression of goat hair coat. By Western analysis, we detected that keratin 33A protein is expressed only in hair coat among the various goat tissues. Moreover, the expression level in winter has increased in cashmere high‐producing Korean native breed, whereas the expression levels between summer and winter had not changed in cashmere low‐producing Saanen. In addition, by immunohistochemistry we determined that keratin 33A is localized in the major cortex portion of cashmere fiber. These results confirm that keratin 33A is a structural protein of goat cashmere hair fiber.     

Anti-atherosclerosis effect of astragaloside is related to miR-33a/ABCA1 signaling

AIM:To study whether astragaloside affects the expression of ATP binding cassette transporter A1 (ABCA1) by regulating miR-33a and promotes the outflow of cholesterol in macrophages. METHODS:In the in vivo experiments, HE staining was used to detect the pathological damage of the cross section of aorta in the mice. The expression of ABCA1 at mRNA and protein levels in mouse aorta was determined by real-time PCR and Western blot. In the in vitro experiments, THP-1 macrophage-derived foam cells were established and then treated with astragaloside-containing serum. Real-time PCR was used to detect the expression of miR-33a. The cells were randomly divided into blank serum group, astragaloside serum group and astragaloside serum+miR-33a mimic group. The expression of ABCA1 at mRNA and protein levels was determined by real-time PCR and Western blot. Oil red O staining and high-performance liquid chromatography were used to detect intracellular lipid content. The method of[³H] incorporation was used to detect intracellular cholesterol outflow. RESULTS:In vivo experiments showed that the blood vessels of the mice in astragaloside group were structurally normal, with neat arrangement, localized small calcified particles, mild lesions, small plaques, reduced foam cells and li-pid, and basically complete elastic plates, indicating that the pathological changes were significantly lighter than those in model group. Compared with model group, the expression of miR-33a in the aorta of the mice in astragaloside group was decreased and the relative expression of ABCA1 at mRNA and protein levels was increased (P<0.05). In vitro experiments showed that astragaloside significantly up-regulated the expression of ABCA1 at mRNA and protein levels, but this effect was inhibited by the transfection of miR-33 mimic without affecting the cell viability. Astragaloside reduced the lipid accumulation in the cells, but this effect was attenuated by miR-33 mimic. Astragaloside reduced intracellular cholesterol accumulation in relation to its promotion of intracellular cholesterol efflux, and the transfection of miR-33a mimic in the cells inhibited cholesterol efflux. CONCLUSION:Astragaloside inhibits the production of miR-33a to increase the expression of ABCA1 and promote the outflow of cholesterol in macrophages. This may be one of the molecular mechanisms of astragaloside in preventing atherosclerosis.     

奶牛瑟氏泰勒虫P33表面蛋白基因的克隆及序列分析

研究根据GenBank上发表的牛瑟氏泰勒虫P33表面蛋白的基因序列设计合成1对引物,用PCR方法扩增出了牛瑟氏泰勒虫的基因片段,将该基因纯化后连接到pMDl8-T栽体上,进行序列测定和分析,并用此方法对延边某奶牛场10头奶牛进行了检测.结果表明:10份样品中9份为阳性,所扩增的目的基因核苷酸长为868 bp,共编码283个氨基酸;该段基因片段与牛泰勒虫韩国株核苷酸序列同源性为99.4%,氨基酸同源性为99.3%.     

牛瑟氏泰勒虫表面蛋白P33基因的克隆与真核表达载体的构建

根据已发表的牛瑟氏泰勒虫(Theileria sergenti)表面蛋白P33基因的核苷酸序列设计引物,应用PCR技术从牛瑟氏泰勒虫基因组DNA中扩增P33基因片段,克隆到pMD18-T simple载体上,重组质粒经PCR、酶切鉴定后测序分析。进一步将该基因插入到真核表达载体pVAXⅠ,为今后深入研究该基因的表达及其功能奠定了基础。     

Effect of Xingnao enema fluid on brain injury and IL-33/ST2 signaling pathway in rats after cardiopulmonary resuscitation

AIM: To study the effects of Xingnao enema fluid on brain injury and IL-33/ST2 signaling pathway in rats after cardiopulmonary resuscitation, and to explore the brain protective effect and mechanism of Xingnao enema fluid. METHODS: SD rats were randomly divided into sham operation group, model group, low-dose (5 mL/kg), middle-dose (10 mL/kg) and high-dose (20 mL/kg) Xingnao enema liquid groups, and ulinastatin group, with 12 rats in each group. Except for the rats in sham operation group, the rats in other groups were used to establish the model of cardiopulmonary resuscitation and were treated with Xingnao enema fluid and ulinastatin. Seven days later, all rats were scored for neurological deficit. The rats were sacrificed, and the water content of brain tissues was calculated. Hematoxylin-eosin (HE) staining was used to detect the pathological changes of brain tissues of the rats in each group. The levels of super-oxide dismutase (SOD) and malondialdehyde (MDA) were detected in the brain tissue. The levels of S100 calcium bin-ding protein beta subunit (S100β) and neuron-specific enolase (NSE) in serum, and interleukin (IL)-1β and tumor necrosis factor (TNF)-α in brain tissue were measured by ELISA. The expression of interleukin-33 (IL-33) and growth stimulation expressed gene 2 (ST2) in brain tissue was determined by Western blot. RESULTS: Compared with sham operation group, the brain tissue of model group showed tissue disorder, focal hemorrhage, neuronal nucleus contraction, apoptosis and other pathological changes, and the neurological deficit score was increased. The water content of brain tissue, the le-vels of S100β, NSE, MDA, IL-1β, TNF-α, IL-33 and ST2 were significantly increased, and the level of SOD decreased significantly (P<0.05). Compared with model group, the pathological damage of brain tissue in low-, middle- and high-dose Xingnao encma fluid groups and ulinastatin group was reduced, and the neurological deficit score was decreased. The water content of brain tissue, levels of S100β, NSE, MDA, IL-1β, TNF-α, IL-33 and ST2 were significantly decreased, and the level of SOD was increased significantly (P<0.05). There was a dose-dependent relationship in different doses of Xingnao enema fluid groups, and no significant difference between high-dose Xingnao enema fluid group and ulinastatin group was observed (P>0.05). CONCLUSION: Xingnao enema fluid repairs brain injury in rats after cardiopulmonary resuscitation, and down-regulation of IL-33/ST2 signaling pathway may be its mechanism.     

高粱不育系QL33A的特征特性及应用分析

QL33A是吉林农业大学农学院1990年从澳大利亚引进的优良高粱不育系。该不育系育性稳定,配合力高,耐旱、耐密、耐瘠薄能力强,抗叶斑病和黑穗病。利用QL33A已育成省级审定品种3个,还有5个新组合正处于区域试验阶段。所配杂交种抗逆性强,适应性广,增产潜力大,对我国春播早熟区的高粱育种和生产起到了较大的推动作用。     

寒地水稻新品种龙粳33的选育

龙粳33是黑龙江省农业科学院佳木斯水稻研究所2002年以空育131为母本,松99-135为父本杂交选育而成优质、高产、抗病、耐冷的水稻新品种。2012年1月通过黑龙江省农作物品种审定委员会审定推广。介绍了龙粳33选育经过、特征特性及栽培要点。     

牛瑟氏泰勒虫套式PCR检测方法的建立及初步应用

根据GenBank上发表的牛瑟氏泰勒虫P33基因序列设计2对特异性引物,建立套式PCR检测方法。该方法与弓形虫RH株、猪附红细胞体和犬新孢子虫均无交叉反应,能检测到的最低DNA量为15 fg。在75份临床样本检测中,62份为阳性,阳性率为82.7%。由此可见所建立的套式PCR检测方法具有较高的敏感性和特异性,可用于牛瑟氏泰勒虫急性感染和隐性感染的早期诊断,为该病的临床检测、流行病学调查、进出口检疫和实验室研究提供了新的技术手段。     

Transport of phosphorus in leaf veins of Vicia faba L.

The plant nutrient phosphorus (P) is spread throughout the plant within the transpiration stream after uptake in the form of phosphate via the roots. Short‐term distribution is therefore strongly dependent on transpiration rather than on sinks, so that P mainly enters adult leaves. The objective of this work was to investigate the transport of phosphate in leaf veins and its distribution within the leaf. Experiments were performed with broad bean (Vicia faba L. var. Scirocco) using radioactively labeled phosphate. In a relatively new approach, the tracer was monitored by a sensitive imaging plate using Fujifilm's Bioimaging Analyzer System. Radioactive label could be monitored in fresh leaves (without fixing) using exposure times of only 5–20 min. For this reason, the method offered the possibility to obtain several subsequent autoradiographic images of the same sample after different feeding times. Phosphate tracer quickly reached small veins, which were then more intensely labeled than the tissue between them. Within the first 15 min, intercostal fields were only slightly stained. After application of phosphate tracer onto the leaf blade, export from the leaf was pronounced and started within 30 min. Phosphorus is effectively redistributed from adult leaves to sinks such as the terminal bud or growing root tips. Using the imaging method, an impression of the high velocity and effectiveness of this process was obtained. The results furthermore imply that leaf veins may be one of the most important locations where exchange between xylem and phloem transport takes place, although exchange between xylem and phloem was also observed in the shoot axis and in the petioles.