橡胶树品种热研7-33-97花药愈伤组织形态发生和组织学研究

以大规模推广的橡胶树品种热研7-33-97为材料,对橡胶树花药愈伤发生的形态学、组织学进行研究,为胚与非胚性愈伤组织的区分提供试验依据,在橡胶树组织培养和遗传转化中具有重要的意义.结果表明,花药胚与非胚性愈伤组织有着明显差别,前者表现为组织疏松脆性,表面凹凸不平,有较多小球状突起,细胞中含有较大的胞核,细胞形状呈规则小圆球形,细胞排列紧密,细胞体积较小;后者存在两种类型,第一种表现为结构紧密,整块组织硬实,表面较平滑,细胞形态不规则,胞质稀少,部分细胞含有极小的胞核,细胞体积大,第二种表现为组织松软、水渍状,细胞不含有胞核,胞质极稀薄并液泡化,细胞形状不规则,细胞排列较疏松,胞间间隙较大,细胞体积较大.热研7-33-97花药胚性愈伤发生率较低,仅为42%,但分化力强.     

核桃炭疽病生防菌yb33的鉴定及其次生代谢物特性分析

为了较好地利用生防菌防治核桃炭疽病,采用土壤稀释平板法和对峙培养法,从核桃根际土壤中分离筛选获得了yb23、yb31、yb33、yb34菌株(其对核桃炭疽病菌的抑菌活性均在60%以上),然后对其中生防效果较好的菌株yb33进行了形态学与分子生物学鉴定,并对该菌次生代谢产物的性质进行了分析。鉴定结果表明:菌株yb33为芽孢杆菌属细菌,其与Bacillus axarquiensis的亲缘关系最近。分析结果表明:不同浓度yb33无菌滤液的抑菌效果间具有明显的差别,其在65℃及其以下温度条件下的热稳定性较好。     

同时高产甘露聚糖肽与乳酶生发酵条件的研究

[目的]探索同时高产甘露聚糖肽和乳酶生的最佳发酵条件。[方法]以甲型溶血性链球菌H1S-33号为菌株,进行液体发酵培养,在各个时间段分别取样测定活菌数及甘露聚糖肽含量,确定最佳培养条件。[结果]在液体发酵过程中,28h发酵液中甘露聚糖肽含量最高为69.2μg/ml,活菌数最多为2.38×1016个/ml。[结论]同时高产甘露聚糖肽与乳酶生发酵条件为：培养基由牛肉膏、蛋白胨、酵母膏、氯化钠组成,发酵温度37℃,最佳发酵时间28h。     

IL-33-modified BMSCs attenuate acute kidney injury induced by sepsis in rats through MyD88

AIM To investigate the effect of interleukin-33 (IL-33)-modified bone marrow mesenchymal stem cells (BMSCs) on sepsis-induced acute kidney injury (AKI) in rats and the expression of myeloid differentiation factor 88(MyD88). METHODS A septic rat model was established by cecal ligation and puncture. The SD rats (n=80) were randomly divided into control group, model group, negative transfection group (transplanting untransfected BMSCs) and IL-33 transfection group (transplanting BMSCs transfected with IL-33), with 20 in each group. Survival rates of the rats within 72 h in the 4 groups were compared. Serum creatinine (SCr) and blood urea nitrogen (BUN) levels were measured before, and 24, 48 and 72 h after transplantation. The kidney pathological damage was observed by HE staining, and the apoptosis of renal cells was detected by TUNEL method 72 h after transplantation. Western blot was used to detect the protein expression levels of interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α), Toll-like receptor 4 (TLR4) and MyD88. RESULTS The survival rate of the rats in model group was significantly lower than that in control group (P<0.05). The survival rate of the rats in IL-33 transfection group was higher than that in model group and negative transfection group (P<0.05). The levels of SCr and BUN in model group were higher than those in control group (P<0.05). The levels of SCr and BUN in IL-33 transfection group were significantly reduced after transplantation, and were lower than those in model group and negative transfection group (P<0.05). The renal tissue pathological injury score in model group was significantly higher than that in control group (P<0.05). Compared with model group and negative transfection group, the renal tissue pathological injury score in IL-33 transfection group was significantly reduced (P<0.05). The proportion of apoptotic cells in the kidney tissues in model group were higher than that in control group (P<0.05). Compared with model group and negative transfection group, the proportion of apoptotic cells in the kidney tissues in IL-33 transfection group was significantly reduced (P<0.05). The protein expression levels of IL-1β, IL-6, TNF-α, TLR4 and MyD88 in model group were significantly higher than those in control group (P<0.05). Compared with model group and negative transfection group, the protein expression levels of IL-1β, IL-6, TNF-α, TLR4 and MyD88 in IL-33 transfection group were significantly decreased (P<0.05). CONCLUSION IL-33 gene-modified BMSCs significantly improve the renal function of AKI rats with sepsis. The mechanism may be related to IL-33 regulating TLR4/MyD88 signaling pathway and inhibiting renal inflammatory response.     

GA信号因子 SlMYB33对番茄开花时间及果实大小的影响

以番茄Micro-Tom为材料,以GA信号转导因子GAMYB基因 SlMYB33为对象,利用GUS组织化学染色分析 SlMYB33在花和果实中的表达模式;其次,通过载体构建、遗传转化得到转基因植株,通过转基因植株的表型分析探究 SlMYB33对番茄开花及果实发育的影响。结果表明, SlMYB33主要在番茄雄蕊和柱头中表达;在番茄Micro-Tom中过表达 SlMYB33,获得OE3、OE4、OE5、OE6和OE7五个过表达株系;表型观察表明, SlMYB33的过量表达可导致番茄开花时间延迟,同时促进果实增大。     

二晚抛栽稻播种量、化控措施与抛栽秧龄的研究

研究表明,随着秧龄的延长,盘育秧的秧苗素质越来越不如湿润秧,主要表现为植株矮小,茎细,单株叶面积小,百苗干重轻,分蘖停止早并出现大量夭亡;稀播能改善秧苗素质,减少串根,采用两次化控控制株高,促进分蘖,减少串根有一定效果,抛栽稻和手插稻比较,在经济性状上表现为穗数增加,每穗粒数减少,结实率与千粒重降低;不同秧龄抛栽,早抛的各项经济性状均比晚抛的好,产量比晚抛的高,特别是秧龄超过３０ｄ抛栽,产量减少明显。最后讨论了二晚抛栽稻的育秧技术。     

鲁垦棉33号在东营的种植表现及其栽培技术

鲁垦棉33号在东营盐碱地棉区大田示范表现综合农艺性状好,高产稳产,抗逆性强,纤维品质优,适宜在黄河中下游流域作为春棉种植。对该品种的栽培技术进行了研究总结。     

青麸杨腺毛结构、化学组分及基因表达解析

通过石蜡切片、组织染色及实时荧光定量技术研究青麸杨腺毛,旨在探究青麸杨腺毛的组织结构、化学组分及基因调控机理。结果表明：青麸杨腺毛呈松塔状,有1个由（12.00 ± 4.14）个分泌细胞构成的头部及无细胞结构的柄部构成,在排出分泌物后,腺毛逐渐萎缩并凋落。腺毛的分泌物中含有大量的粘性多糖、酚类物质及酸性脂质,这些分泌物在为幼嫩的叶片提供物理保护层,防止水分丧失的同时,还可有效防止病原微生物在幼嫩叶片表面大量繁殖。通过转录组分析结合实时荧光定量PCR验证,鉴定出在腺毛中特异表达的候选关键基因,其中,与植物—病原菌互作相关的基因WRKY33和PTI6,及参与调控对昆虫有毒副作用的氰基氨基酸代谢的基因SLC36A在腺毛中的表达量较叶片中均显著上调。     

水稻叶早衰突变体es33的鉴定和基因定位

利用甲基磺酸乙酯(EMS)化学诱变单季常规晚粳稻浙粳99,获得叶早衰突变体es33。为揭示es33突变体早衰的分子调控机制,以野生型浙粳99为对照,对突变体进行表型鉴定,统计农艺性状和测定光合性能,并对早衰基因ES33进行定位。结果表明,突变体es33幼苗在播种后第10天,第2和第3叶的叶尖出现明显干枯早衰现象,与野生型相比,分蘖盛期叶片光合色素含量降低、叶绿体结构疏松、光合作用减弱、部分酶活性降低,成熟期植株矮小、分蘖少、衰老严重。遗传分析结果表明,突变体es33早衰性状受一对隐性核基因控制,利用基因定位和重测序技术,将控制该性状的基因定位在水稻第3号染色体上的SSR标记STS-15和G24之间,物理距离为977.8 kb,经测序确定LOC_Os03g31550为候选基因;ES33基因第11个外显子发生4个碱基的缺失,造成移码突变,导致蛋白(黄嘌呤脱氢酶)翻译提前终止;通过系统发育树分析和氨基酸序列比对得知,ES33蛋白在禾本科作物中高度保守。本研究结果为进一步探究早衰基因ES33的功能奠定了基础。