台湾南元花园休闲农场体验活动之检视与分析

台湾南元花园休闲农场除拥有其自身之农场特色外,在经营管理上更有其独到的一面,该文以台湾南元花园休闲农场为个案研究对象,调查其农场基础资源与检视其现有之体验活动,并由调查结果中分析其现有之体验活动是否善用期农场基础资源与符合其农场经营方向,最后将研究结果提供给休闲农场业者在设计体验活动设计时之参考,以提高体验活动的经济价值。     

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on T_m values of 85.03 ± 0.54℃ for stx1 and 87.47 ± 0.35℃ for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/µL), and quantifiable (R² = 0.9313). The efficacy and sensitivity were measured to evaluate applicability of the mLAMP assay to cattle farm samples. A total of 12 (12/253; 4.7%) and 17 (17/253; 6.7%) STEC O157, and 11 (11/236; 4.7%) non-O157 STEC strains were isolated from cattle farm samples by conventional selective culture, immunomagnetic separation, and PCR-based culture methods, respectively. The coinciding multiplex PCR and mLAMP results for the types of shiga toxin revealed the value of the mLAMP assay in terms of accuracy and rapidity for characterizing shiga toxin genes. Furthermore, the high detection rate of specific genes from enrichment broth samples indicates the potential utility of this assay as a screening method for detecting STEC in cattle farm samples.     

猪场建设中“水”问题的探讨

给水及排水是在猪场规划建设时就应该统筹考虑的重大问题。从给水工程设计和废水处理两方面探讨了猪场建设中所涉及的"水"问题,介绍了水源、水量、水质、水温控制、施工要点、废水处理方式等诸多细节,以供同行参考。     

Phage and MLVA Typing of Salmonella Enteritidis Isolated from Layers and Humans in Belgium from 2000–2010, A Period in which Vaccination of Laying Hens was Introduced

The aim of the study was to characterize isolates of Salmonella enterica serovar Enteritidis (S. Enteritidis) obtained from humans and layer farms in Belgium collected during 2000–2010. Three periods were compared, namely (i) before implementation of vaccination (2000–2004), (ii) during voluntary vaccination (2005–2006) and (iii) during implementation of the national control program (NCP) for Salmonella including mandatory vaccination against S. Enteritidis (2007–2010). The characteristics compared across time periods were distributions of phage type and multiple‐locus variable number tandem‐repeat assay (MLVA). While PT4 and PT21 were predominantly isolated in Belgium in layers and humans before 2007, a significant reduction of those PTs was observed in both populations in the period 2007–2010. The relative proportion of PT4b, PT21c and PT6c was found to have increased considerably in the layer population since 2007. In the human population, PT8, PT1 and the group of ‘other’ PTs were more frequently isolated compared to the previous periods. When comparing the proportion of the predominant MLVA types Q2 and U2, no significant difference was found between the layer and human population in the three periods and between periods within each category (layer and human). A significant difference in isolate distribution among MLVA clusters I and II was found between human and layer isolates recovered during Period 3 and in the human population between Period 1 and 3. Results suggest that the association between S. Enteritidis in layers and the occurrence of the pathogen in humans changed since implementation of the NCP in 2007.     

织篢农场开展从严治党主题教育活动实践的思考

结合织篢农场工作实际,从4个方面介绍农场开展从严治党主题教育活动实践。总结出用好“群众路线”法宝、占领理想信念教育制高点、抓住严格党内生活关键点和抓好从严治吏着力点等好做法。教育活动管好了党员干部,提高职工生产的积极性,提升了职工收入和农场橡胶产量。     

场次降雨条件下考虑田梗高度的农田产流规律模拟

条块分割、田埂密集是海河平原农田的典型特征,科学认知田埂作用下的农田产流规律对解析平原区水循环演变过程至关重要。目前,流域尺度的农田产流规律的研究较少,关于降雨特征-田埂高度-农田产流三者之间的影响关系还不明晰,田埂作用下的农田产流规律是平原区水循环演变解析的薄弱环节。该研究基于CMOPRH遥感降水数据产品分析了海河平原场次降雨特征,并以Smith & Parlange入渗模型为核心构建农田积水产流模型,模拟海河平原降雨-产流过程。结果表明：1）海河平原场次降雨总量以小于25 mm为主,占降雨总场次的95%,10 h以内的场次降雨占比为96%,降雨总量和降雨强度由东北部向西北部递减;2）在2008-2019年降雨条件下,模拟无田埂、10 cm田埂、12 cm田埂、15 cm田埂四种情景的农田年均产流量,结果分别为62.4亿m3/a、19.7亿m3/a、13.1亿m3/a、5亿m3/a;3）田埂高度已知的条件下,降雨历时和降雨强度是影响农田产流的关键因素,以降雨历时为横坐标、降雨强度为纵坐标,可以通过拟合反比例函数曲线划分一场降雨是否产流;4）根据海河平原历史农田面积变化估算,2000年海河平原农田产流量相对1979年减少10.2亿m3,2016年农田产流量相对2000年减少5.1亿m3。该研究定量解析了降雨特征、田埂高度对农田产流的影响,为解析海河平原区地表水资源衰减原因提供了重要的规律认知。     

杉木3代种子园良种与1.5代种子园良种营建林对比试验

以杉木3代种子园和1.5代种子园良种营建对比试验林,对试验林11、21、34、45个月生的生长量进行调查分析,结果表明：杉木3代种子园良种生长显著优于1.5代种子园良种。其林分45个月生时,平均树高、胸径、单株立木材积分别为4.26m、5.97㎝、0.0083m3,分别比对照大53.24%、88.92%、336.84%,且林分整齐性优于杉木1.5代种子园良种。     

对广西国有林场文化建设的探讨

国有林场文化是国有林场在长期生产经营活动中创造的独具特色的精神财富和物质财富,它包含林场广大员工在长期经营活动中形成并共同遵守的价值信念、行为规范及最高目标.国有林场文化在林场的发展壮大中起着越来越重要的作用.广西国有林场文化建设有其自身的特点.广西国有林场应着力打造林场的核心文化,积极推进廉政文化,重视生态文化,不断丰富娱乐文化,大力加强学习文化,形成新的富有特色的林场文化.     

江苏省基本农田土地整治项目土地平整计算方法探讨

土地平整工程是土地整治项目的核心内容。该研究针对江苏省大多数情况下基本农田土地整治项目的土地平整进行了初步探讨,并提出一种简便的计算方法以供参考。     

吉水县芦溪岭林场发展现状的思考

对芦溪岭林场现状进行分析,指出在建设、发展、改革中,林场存在着森林资源结构不合理、内部管理不够规范、经营管理机制不活、发展环境脆弱等问题。由此提出,林场必须大力培育森林资源,以人为本强化内部管理体制,协调各方关系创造良好的周边发展环境等对策,从而实现对林场森林资源的保护和林场经济的可持续发展。