全文获取类型
收费全文 | 279篇 |
免费 | 12篇 |
国内免费 | 16篇 |
专业分类
林业 | 19篇 |
农学 | 45篇 |
基础科学 | 3篇 |
39篇 | |
综合类 | 137篇 |
农作物 | 21篇 |
水产渔业 | 3篇 |
畜牧兽医 | 5篇 |
园艺 | 17篇 |
植物保护 | 18篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 7篇 |
2020年 | 8篇 |
2019年 | 10篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 15篇 |
2015年 | 14篇 |
2014年 | 13篇 |
2013年 | 24篇 |
2012年 | 19篇 |
2011年 | 26篇 |
2010年 | 19篇 |
2009年 | 15篇 |
2008年 | 26篇 |
2007年 | 17篇 |
2006年 | 10篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
排序方式: 共有307条查询结果,搜索用时 15 毫秒
31.
以红薯[Ipomoea batatas(L.) Lam]茎叶为原料,采用酶-超声波提取绿原酸.对料液比、加酶量、超声功率和超声时间进行了单因素和正交试验.确定绿原酸提取的最佳工艺为料液比1∶20、加酶量1.5%、超声波功率175W、提取时间20 min,在此条件下,绿原酸的提取率为3.45%. 相似文献
32.
白骨壤为红树林植物中的广布种、先锋树种,其沙地育苗技术尚未见报道,文章结合白骨壤的沙地育苗,总结了种子采集及处理、育苗基质、播种、苗期管理和炼苗出圃等技术要点. 相似文献
33.
用响应面法优化甘薯(Ipomoea batatas)叶黄酮的微波提取工艺,研究了微波功率、微波时间、乙醇体积分数、液固比4个因素对黄酮提取率的影响.并采用Box-Behnken设计对最佳提取工艺进行了优化.结果表明,最佳工艺条件为微波功率200 W、微波时间4 min、乙醇体积分数50%、液固比43∶1(V/m,mL∶g),在此条件下甘薯叶黄酮的提取率为8.082 7%,与预测值8.314 2%基本吻合. 相似文献
34.
35.
36.
Jinxi HUO Bing DU Sifan SUN Shaozhen HE Ning ZHAO Qingchang LIU Hong ZHAI 《农业科学与工程前沿(英文版)》2018,5(2):206
High concentrations of Cd can inhibit growth and reduce the activity of the photosynthetic apparatus in plants. In several plant species, aldo-keto reductases (AKRs) have been shown to enhance tolerance to various abiotic stresses by scavenging cytotoxic aldehydes; however, few AKRs have been reported to enhance Cd stress tolerance. In this study, the gene IbAKR was isolated from sweet potato. The relative expression levels of IbAKR increased significantly (approximately 3-fold) after exposure to 200 mmol·L−1 CdCl2 or 10 mmol·L−1 H2O2. A subcellular localization assay showed that IbAKR is predominantly located in the nucleus and cytoplasm. IbAKR-overexpressing tobacco plants showed higher tolerance to Cd stress than wild-type (WT). Transgenic lines showed a significant ability to scavenge malondialdehyde (MDA) and methylglyoxal (MG). In addition, proline content and superoxide dismutase activity were significantly higher and H2O2 levels were significantly lower in the transgenic plants than in the WT. Quantitative real-time PCR analysis showed that the reactive oxygen species (ROS) scavenging genes encoding guaiacol peroxidase (GPX), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR) and peroxidase (POD) were significantly upregulated in transgenic plants compared to WT under Cd stress. These findings suggest that overexpressing IbAKR enhances tolerance to Cd stress via the scavenging of cytotoxic aldehydes and the activation of the ROS scavenging system. 相似文献
37.
Interaction of plant genotype and management in the persistence of a perennial grass exposed to grazing and soil fertility stresses
下载免费PDF全文
![点击此处可从《Grass and Forage Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Increased stocking rates under typical pasture improvement practices in southern Australia have often been associated with low persistence by sown perennial grasses subjected to continuous stocking. Cultivars of the perennial grass phalaris (Phalaris aquatica L.), which is widely used in southern Australia, vary in their phenology and growth habit. Winter‐active cultivars have also been developed with improved stress tolerances, viz. heavy grazing pressure (Holdfast GT) and acid soils (Advanced AT). This study examined the persistence of phalaris genotypes over 4 years under five management treatments: high P‐high stocking rate (18 sheep per hectare) rotationally stocked (HPRS); high P‐high stocking rate continuously stocked (HPCS); high P‐intermediate stocking rate (13·5 sheep per hectare) continuously stocked (HPCS13.5); low P‐low stocking rate (9 sheep per hectare) rotationally stocked (LPRS); and low P‐low stocking rate continuously stocked (LPCS). Basal frequency of phalaris declined rapidly under HPCS and LPCS in the first year of treatments when periods of low herbage mass occurred under low rainfall. Basal frequency in high P treatments stabilized and partially recovered but continued to decline in low P treatments. Holdfast GT survived the early decline in the HPCS treatment better than other winter‐active cultivars and similar to a known grazing tolerant, semi‐winter‐dormant cultivar. Advanced AT and older winter‐active cultivars were sensitive to continuous stocking but persisted better under rotational stocking or under a reduced rate of continuous stocking (HPCS13.5). Rotational management clearly promoted persistence of winter‐active phalaris, but long‐term persistence will be better ensured by combining management with the use of genotypes with grazing tolerance traits. 相似文献
38.
39.
紫甘薯花色苷的组分及抗氧化活性研究(英文) 总被引:2,自引:0,他引:2
对紫甘薯花色苷的化学成分和抗氧化活性进行了研究.研究采用大孔树脂AB-8纯化紫甘薯花色苷,高效液相色谱-2极管阵列法(HPLC-DAD)分析表明,纯化后的提取物中共含有11种花色苷,其中主要成分为酰化的矢车菊素和芍药素.并测定了紫甘薯总花色苷在DPPH自由基清除体系、超氧阴离子体系、还原力和亚油酸体系的抗氧化活性.在质量浓度均为0.5g/L时,花色苷、L-AA和 BHT的还原力分别为0.572、0.460 和0.121,花色苷的清除DPPH自由基的半数抑制浓度(IC50)和清除超氧阴离子IC50分别为6.94和3.68mg/L,表明花色苷还原能力强,并能有效地清除DPPH自由基和超氧阴离子.此外,紫甘薯花色苷能较好地抑制脂质过氧化. 相似文献
40.
30个中国甘薯主栽品种的RAPD指纹图谱构建及遗传变异分析 总被引:2,自引:1,他引:1
研究以中国30个甘薯主栽品种为材料,对甘薯RAPD指纹图谱的构建进行了探讨。从102个RAPD引物中筛选出26个多态性丰富的引物用于PCR扩增,共扩增出255条多态性条带,平均每个引物扩增的多态性带数为9.8条。其中S32和S39多态性最高,仅用其中1个引物即能将这30个甘薯品种完全区分开,且由此构建的指纹图谱出现的概率很小,分别为4.77×10^-7(1/221)和1.91×10^-6(1/219)。结果进一步表明,这30个甘薯品种的遗传距离变异幅度较大,为0.0390~0.4306,平均遗传距离为0.3086。RAPD聚类分析表明,地域分布相近的甘薯品种和具有同一亲本的甘薯品种聚在一起,与这些甘薯品种的系谱图一致。 相似文献