靶向G-四链体的抗番茄丛矮病毒卟啉类衍生物的筛选

为寻找具有高活性抗病毒化合物,开发绿色抗植物病毒剂,设计合成了21个α-羟基-γ-丁内酯类化合物,其结构经过核磁共振氢谱、碳谱以及高分辨质谱确认,并初步测定了它们对烟草花叶病毒 (TMV) 的钝化活性、保护活性和治疗活性。结果表明：该类化合物具有较好的抗TMV活性,其中化合物 8o 表现出显著的钝化活性,其EC₅₀值为226.2 μg/mL,优于对照药剂病毒唑 (EC₅₀ 值308.4 μg/mL)。     

不同月龄纯种和牛主要血液指标变化规律的研究

目前我国对和牛血液指标基本情况的报道较少,本研究测定并对比了不同月龄纯种和牛血液指标变化规律。结果表明,不同月龄纯种和牛血清中β-胡萝卜素含量在1.29~2.64μg/mL之间,γ-谷氨酰转移酶(γ-GTP)含量在19.95~26.81UI/L之间,维生素A含量在37.00~56.74ng/mL之间。     

MDCK细胞中IFN-γ诱导的免疫相关GTP酶在弓形虫PVM表面的定位研究

为探究犬MDCK细胞中γ-干扰素(IFN-γ)诱导的免疫相关的GTP酶在刚地弓形虫(T.gondii)的纳虫空泡膜表面的定位情况,本研究采用慢病毒包装载体将免疫相关的GTP酶(Irgb11)与GFP的融合基因(Irgb11-GFP)引入犬MDCK细胞中,经荧光鉴定和western blot检测到Irgb11-GFP融合蛋白的表达,表明构建了过表达Irgb11的细胞系。采用IFN-γ诱导该细胞系24h后,接种弓形虫PLK株,以间接免疫荧光试验检测,并通过激光共聚焦扫描显微镜观察,结果显示Irgb11-GFP蛋白获得表达并能够与弓形虫PLK株的纳虫空泡膜(PVM)发生共定位,表明在IFN-γ存在的情况下,犬MDCK细胞中的Irgb11可以被募集到弓形虫PVM表面,从而发挥其破坏弓形虫PVM的功能。本实验为进一步研究犬细胞中抑制弓形虫生长的机制奠定基础。     

Evaluation of the sensitivity to ionising γ-radiation of a Cymbidium hybrid

Gamma-rays are an important mutagenic agent that can induce new, useful genetic variations in plants. However, γ-irradiation can also cause damage that negatively affects the use of such mutagens in plant breeding. The aim of the present study was to investigate the effects and damage caused by γ-radiation in a Cymbidium hybrid, RB001. The relative growth rate of protocorm-like bodies (PLBs) was reduced by 50% at a γ-ray dose of approximately 40 Gy. Malondialdehyde concentrations increased significantly with increasing radiation dose. However, almost no difference was observed between untreated control PLBs and PLBs treated with 200 Gy 8 weeks after γ-irradiation. The activities of several antioxidant defence enzymes increased gradually with increasing γ-ray dose, 24 h after irradiation. These enzymes showed different responses between 1 and 4 weeks, but no difference 8 weeks after irradiation. The ‘comet assay’ and flow cytometry were performed. Clear differences in radiation-induced damage were observed between control and 200 Gy-treated PLBs at 24 h. However, PLBs had a tendency to recover from 4 weeks after irradiation, and the integrity of their DNA was similar in samples treated with 10–200 Gy. These results indicated that γ-rays caused little DNA damage and the plants could recover. This demonstrated the feasibility of using physiological responses, the ‘comet assay’, and flow cytometry to detect DNA damage after γ-irradiation.     

甲酯化对γ-亚麻酸紫外吸收的影响

[目的]考察甲酯化处理对γ-亚麻酸紫外吸收的影响。[方法]利用紫外分光光度法测定甲酯化前后多种γ-亚麻酸产物的吸光度,并结合气相色谱法检测γ-亚麻酸含量,对结果进行相关性分析。[结果]甲酯化前后的紫外检测结果之间的相关性达到0.01显著水平(P<0.01)。[结论]对γ-亚麻酸等多烯不饱和脂肪酸进行紫外吸收检测时,可以不再经过甲酯化处理。     

水稻谷氨酸脱羧酶基因(OsGAD3)分子进化与表达分析

利用分子克隆技术从8个水稻品种中克隆到谷氨酸脱羧酶基因OsGAD3,并进行了相关的生物信息学及表达谱分析.结果表明,不同水稻品种OsGAD3的核苷酸序列差异性很小,都在1%以内,在氨基酸序列上都具有保守的结构域,在C末端均具有类似于OsGAD1结合CaM的关键氨基酸位点,但不同于OsGAD2.系统进化树分析表明,不同水...     

γ射线辐射诱变抗杨锈病的研究

本研究以”禾阳2”杨为辐射诱变材料,研究了用γ射线(60Co)辐射诱变在培养基上定向筛选抗病变异体的方法.试验比较了茎段、叶片和叶柄等不同器官材料诱导胚性愈伤组织的效果,发现以茎段的胚性愈伤诱导率最高,达到100％；愈伤分化培养46h后开始有胚性细胞分化,74～92 h有大量胚性细胞形成,是进行γ射线辐射处理的最佳时期...     

甘蔗新品种“海大28号”的选育及其繁殖推广

甘蔗新品种“海大28号”是广东海洋大学农业生物技术研究所甘蔗研究室从母本为Co419、父本为Cp49／50的杂交种子播种育苗，按甘蔗育种程序逐级试验筛选获得的优良单系90／158，经采用^60Coγ射线对90／158单系梢部苗的单芽和茎梢幼嫩组织诱导的愈伤组织进行辐射处理改良而育成甘蔗新品种。多年试验结果表明，该品种具有高产、高纤维分、高糖、耐旱、抗虫、抗寒的糖能兼用的甘蔗新品种，适合广东、广西、云南、海南等蔗区地力中等或中等以上的旱坡地及水旱田推广种植。     

Role of IFN-α during the acute stage of a swine influenza virus infection

Cytokines, especially interferon-alpha (IFN-α) are important in controlling influenza virus infections. To investigate the role of IFN-α in influenza, the swine IFN-α neutralizing monoclonal antibody (Ab) K9 was applied in a swine model of influenza A virus infection. First, the optimal dose and route for administration of the IFN-α neutralizing Abs was determined. Based on those results, the effect of the Abs on a swine influenza virus infection was investigated. Pigs were inoculated intratracheally with 10^6.0 mean egg infectious dose (EID₅₀) A/Swine/Belgium/1/98 (H1N1) virus. At the time of challenge and 18 h later, they were injected intratracheally and intraperitoneally with a high dose of IFN-α neutralizing Abs or control Abs. The animals were euthanized at 0, 24, 30, 48 and 72 h after inoculation. At 24 and 30 h, IFN-α levels in broncho-alveolar lavage fluid of K9 recipient animals were strongly suppressed, and this coincided with reduced IL-6 and IL-12 levels. TNF-α and IL-1 levels were unaffected compared to those in the control Ab treated group. Importantly, the onset and peak of clinical symptoms in IFN-α neutralizing Abs treated animals were delayed by 24 h, simultaneously with the suppression of IFN-α, but there was no obvious effect on virus replication and lung pathology. These results suggest an important role for IFN-α in IL-6 and IL-12 induction and a role of all three cytokines in the symptoms of swine influenza.