自由基清除剂对传染性法氏囊病发病过程的影响

本试验用自由基清除剂超氧化物歧化酶和还原型谷胱甘肽分别处理实验性传染法氏囊病患鸡，以探讨自由基清除剂对ＩＢＤ发病过程的影响。结果表明ＳＯＤ和还原型谷胱甘肽自理过的ＩＢＤ患鸡，其临床症状，病理变化和凝血功能障碍均比攻毒对照组明显减轻。     

鸡传染性法氏囊病细胞弱毒苗的研制

采用ＩＢＤＶ－Ｄ＿（７８）株种毒，由细胞培养研制的ＩＢＤ弱毒疫苗，经安全试验和免疫试验，证明适合本地流行毒株，试验免疫保护率１００％，对照鸡均发病。     

抗传染性法氏囊病病毒单克隆抗体的研究 Ⅱ．AC─ELISA方法的建立

将所制备的腹水单抗ＡＢ７、ＢＧ９经硫酸铁盐析、透析，再经ＤＥＡＥ－纤维素柱层析纯化后，采用戊二醛二步法用辣根过氧化物酶标记单抗，建立了用于鉴别传染性法氏囊病病毒抗原型的ＡＣ－ＥＬＩＳＡ方法，并对国内的６个分离株及美国变异株１０８４Ａ进行了检测，结果表明，上述国内分离株均含有ＣＪ８０１株相一致的ＡＢ７、ＢＧ９位点，而１０８４Ａ株缺失ＡＢ７位点。     

鸡肾型传染性支气管炎的诊断与防治

甘鸡场鸡发生以呼吸道及肾病变为主的疾病经流行病学调查、病理剖检、病毒分离、电镜观察生物学特性及理化特性试验，血清中和试验，动物回归等确诊为肾型传染性支气管炎。采用分离毒鸡组织灭活苗结合Ａｕｓｔ－Ｔ株灭活油乳苗有效地防治了该病。     

A comparison of different models for assessing variations in the sero-prevalence of infectious bovine rhinotracheitis by farm, area and district in Kenya

The relative variability of the sero-prevalence of antibodies to infectious bovine rhinotracheitis (IBR) due to cow, farm, and agroecological area levels were investigated for three contrasting districts in Kenya: Samburu, an arid and pastoral area; Kiambu, a tropical highland area; and Kilifi, a typical tropical coastal area. Cattle were selected by two-stage cluster sampling and visited once between August 1991 and 1992. Data on animal, farm, and area factors were analyzed using Schall's algorithm and MLn (multi-level, n-level), two generalized mixed-model programs suitable for multi-level analysis. Most variation in IBR sero-prevalence was from farm-to-furm. This was reflected by the many farm-level fixed effects (farm size, disease control measures and type of breeding) significant in models both ignoring and accounting for single variance components (clustering) at farm, area, and district levels. Area-to-area and district-to-district variations were noted but the area and district variance components were one-third and one-fifth the size of the farm variance components for both methods. As farm-to-farm variation differed markedly by farm size and district, models in MLn were extended to allow for multiple farm-level variance components by these categories. For each, sero-prevalence of IBR increased with age and was significantly decreased on small-sized zero-grazing farms. These models, particularly the model with different farm variance components by districts, fit the data better and highlighted well that there was considerable farm-to-farm variation—differing by district—and that the available farm-level fixed effects did not predict IBR sero-prevalence well.     

中药抗病毒冲剂对鸡人工混合感染鸡副嗜血杆菌和传染性支气管炎病毒的疗效观察

试验观察了中药抗病毒冲剂对鸡人工混合感染鸡副嗜血杆菌和传染性支气管炎病毒后的治疗效果。结果显示该药具有抗病毒消炎作用,且治愈率分别比对照组高15.4%和10.2%,死亡率比对照组低6.7%,该药可用于治疗由IBV和鸡副嗜血杆菌引起的呼吸道病。     

传染性法氏囊病毒免疫原基因在毕赤酵母中的高效分泌表达

从国内分离的鸡传染性法氏囊病毒（IBDV）众多流行株中获得超强毒株（vvIBDV）,应用RT-PCR方法,扩增得到IBDV主要保护性抗原VP2基因的开放编码框,克隆至巴斯德毕赤酵母表达载体pPICZαA,将该重组质粒pPICZαA-VP2以电转化方法转入毕赤酵母X-33感受态细胞中,通过PCR鉴定、表型筛选和抗生素浓度梯度筛选,获得高拷贝阳性重组酵母菌株X-33-pPICZαA-VP2。经摇瓶诱导表达试验获得稳定高效分泌表达的酵母工程菌,表达量为0.459mg/ml,聚丙烯酰胺凝胶电泳（SDS-PAGE）、免疫印迹（Western-blot）以及动物试验表明,重组酵母工程菌表达的目的蛋白具有鸡传染性法氏囊病病毒天然蛋白的生物活性和免疫原性。     

间接血凝试验检测鸡传染性法氏囊病抗体研究

本试验用鸡传染性法氏囊病(IBD)弱毒抗原致敏绵羊红细胞(SRBC),通过间接血凝试验(IHA)检测了10份接种 IBD 疫苗后第35日龄鸡的血清,10份高免血清,10份未免疫健康鸡血清,并辅以对流免疫电泳(CIEP)相对照。结果 IHA 与 CIEP 的阳性符合率为94.7％,并通过鉴别试验和间接血凝抑制试验,证明此法具有很高的特异性。     

鸡传染性鼻炎灭活油乳剂苗研究

本研究用司本—８０和硬脂酸铝加入白油为油相，吐温—８０加入灭活的副鸡嗜血杆菌液为水相，制备成油乳剂苗．该苗属油包水类型，４℃放置６个月稳定，５倍量接种产蛋鸡，结果无不良反应。该苗免疫鸡群后一个月，其凝集抗体效价为１：６４～１：１２８，攻毒保护率为１００％，免疫期６个月．