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91.
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将硫酸庆大霉素与大肠埃希菌血清抗体用化学修饰剂进行偶联,再经适当的化学方法处理,按照制剂学原理制备成靶向硫酸庆大霉素,经电镜检测,可以清楚的看到抗体球蛋白上附着的硫酸庆大霉素药物颗粒。应用间接ELISA法检测靶向硫酸庆大霉素结合物的免疫原性。结果表明,结合物存在微弱的免疫原性,但与大肠埃希菌血清抗体相比,免疫原性已经大为减弱,说明靶向硫酸庆大霉素复合物中,化学修饰剂不仅扮演偶联物的角色,还减弱了结合物中大肠埃希菌血清抗体的免疫原性,对靶向硫酸庆大霉素结合物的药动学和药效学几乎无影响。 相似文献
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基于协调度模型的青藏高原社区畜牧业生态、社会及经济耦合发展 总被引:2,自引:0,他引:2
为研究青藏高原社区畜牧业生态、社会与经济协调发展程度,通过构建社区畜牧业协调发展综合评价指标体系,在生态、社会、经济3个子系统综合评价的基础上,建立青藏高原社区畜牧业生态、社会、经济耦合协调模型,分析2013-2016年青藏高原社区畜牧业协调发展程度。研究表明,青藏高原社区畜牧业生态、社会、经济的耦合协调度不断提高,且社区间存在一定差异。羊八井社区协调发展水平较低,仍处于不协调状态;墨竹工卡社区、夏河社区及香格里拉社区等5个社区已实现基本协调;红原社区与河南社区耦合协调水平最高,已实现协调发展态势。 相似文献
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为了研究氟对小鼠附睾结构形态及紧密连接相关基因的影响,将60只健康雄性小鼠随机分成4组,对照组饮用蒸馏水,3个氟处理组分别饮用含氟化钠(NaF) 25、50和100 mg/L的蒸馏水。染氟60 d,取小鼠股骨,采用氟离子选择电极法测其氟含量;取右侧附睾制作石蜡切片,HE染色后进行组织形态结构观察;左侧附睾提取RNA,采用实时荧光定量PCR技术(qRT-PCR)检测紧密连接相关基因的表达。结果显示,与对照组相比,氟处理组小鼠股骨氟含量随摄氟剂量的增加呈剂量依赖性,其中50、100 mg/L剂量组差异极显著(P<0.01);氟处理组附睾尾管腔厚度极显著增厚(P<0.01);紧密连接相关基因Claudin-2 mRNA表达量极显著下降(P<0.01),100 mg/L剂量组ZO-1 mRNA表达量显著下降(P<0.05)。摄入过量NaF影响附睾形态及其紧密连接相关基因的表达,从而可能对血-附睾屏障造成损伤。 相似文献
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Liesbet Ledeganck Kaatje Kromhout Koen Chiers Elke Van der Vekens Luc Van Ham Sofie F.M. Bhatti Ine Cornelis 《Veterinary radiology & ultrasound》2023,64(2):E10-E13
A 9-month-old male entire Doberman Pinscher presented with acute onset of severe cervical hyperesthesia after a fall. Neurological examination revealed a normal gait with low head carriage and severe cervical hyperesthesia. A CT scan of the cervical vertebral column revealed the presence of a comminuted fracture at the dorsomedial aspect of the right occipital condyle and sclerosis of the underlying bone. Medical management was initiated consisting of an external bandage, strict rest, and pain medication. Due to the lack of clinical improvement, the dog was euthanized 2 months after diagnosis. Histopathology of the lesion was compatible with a healing fracture. 相似文献
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R. Chiocchetti C. Bombardi C. Mongardi Fantaguzzi D. Russo E. Venturelli C. Montonerl A. Spadarl N. Romagnoli A. Grandis 《Equine veterinary journal》2009,41(8):759-764
Reason for performing study: In horses, morpho‐functional studies related to the enteric nervous system (ENS) controlling the sphincters are lacking. Objectives: To investigate immunohistochemically the morphology, distribution, density, phenotypes and projections of neurons controlling the ileocaecal junction (ICJ). Methods: Two young horses were anaesthetised and underwent midline laparotomy. The neuronal retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of the ICJ. A post surgical survival time of 30 days was used. Following euthanasia, the ileum and a small portion of caecum were removed. Cryosections were used to investigate the immunoreactivity (IR) of the neurons innervating the ICJ for choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), substance P (SP), calcitonin gene‐related peptide (CGRP) and neurofilament NF200kDa (NF). Results: Ileal FB‐labelled neurons innervating the ICJ were located in the myenteric plexus (MP) and submucosal plexus (SMP) up to 48 cm and 28 cm, respectively, from the point of the FB injections. Descending MP and SMP neurons were nitrergic (54 ± 11% and 68 ± 4%, respectively), cholinergic (60 ± 19% and 82 ± 11%, respectively), NF‐IR (54 ± 9% and 78 ± 21%, respectively), and SP‐IR (about 20% in both the plexuses). CGRP‐IR was expressed only by SMP descending neurons (45 ± 21%). In both the plexuses descending neurons coexpressing nNOS‐and ChAT‐IR were also observed (25 ± 11% and 61 ± 27%, respectively). Conclusions: The presence of ileal long projecting neurons innervating the ICJ suggests that they are critical for its modulation. Consequently, in bowel diseases in which the resection of the terminal jejunum and proximal ileum are required, it is preferable, whenever possible, to conserve the major portion of the ileum. Potential relevance: The knowledge of the phenotype of ENS neurons of the ileum might be helpful for developing pharmaceutical treatment of the ICJ motility disorders. 相似文献
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