广东省主导品种广 8 优 165 在肇庆市的推广应用

在肇庆市鼎湖、高要、四会、怀集、封开、德庆、广宁等县(市、区)建立广8优165推广应用示范点,并采用多种形式对广8优165进行宣传推广,促进广8优165在肇庆市的推广应用。肇庆市怀集县岗坪镇兴义村田间现场测产结果显示,广8优165比博优998增产412kg/hm~2,增产率为5.19%;广8优165比湛优226增产558kg/hm~2,增产率为7.03%。据统计,2013—2017年广8优165在肇庆市累计推广应用面积达28067hm~2,平均产量7210kg/hm~2,生产广8优165优质稻谷总产量202277t,创造总经济效益68774万元、新增纯收益4316万元,经济效益十分显著。广8优165十分适合肇庆市的气候环境条件,适宜在全市范围内大面积推广,广8优165的推广应用将促进肇庆市优质水稻产业的健康稳定发展,提高水稻种植的产量和效益。     

Role of endogenous angiotensin II in the pathogenesis of aortic calcification in rats

AIM: To explore the effects of angiotensin II on aortic calcification in the rat. METHODS: Arterial calcification of Sprague-Dawley rats was induced by vitamin D₃ plus nicotine. Calcification was confirmed by Von Kossa staining, measurement of calcium content, [⁴⁵Ca²⁺]accumulation and alkaline phosphatase (ALP) activity of vascular tissue. RESULTS: The results showed that calcium content, [⁴⁵Ca²⁺]accumulation and ALP activity in calcified arteries increased significantly compared with those of control. Ang Ⅱ levels in plasma and aortic tissues and the amount of angiotensinogen mRNA in calcified aorta were also increased as compared with control. Captopril (inhibitor of ACE) and losartan (Ang Ⅱ receptor inhibitor) decreased significantly the content of calcium, [⁴⁵Ca²⁺] uptake and ALP activity in calcified aorta. Ang Ⅱ levels in plasma and aortic tissues and the amount of angiotensinogen mRNA in aortic tissue were down-regulated by captopril. The amount of angiotensinogen mRNA and the content of Ang Ⅱ in the calcified aorta were also decreased by losartan. CONCLUSION: The captopril and losartan significantly alleviate the vascular calcification.     

放射免疫法快速检测鸡肉中青霉素类药物残留

主要探讨应用Charm Ⅱ放射免疫分析方法检测鸡肉中青霉素类药物残留。确定制样和控制点设定的方法,评价了免疫反应体系的灵敏度和特异性,验证了青霉素类药物最大残留限量的检测稳定性。90min可出检测结果。     

三系杂交水稻Ⅱ优7954高产优质制种技术

介绍了II优7954及其亲本的特征特性,并从制种基地的选择、播期安排、种子处理、育秧管理、适时移栽、花期预测与调节、人工授粉、病虫害综合防治、及时除杂等方面对其在六安地区的高产制种技术进行了总结.     

两株Ⅱ型草鱼呼肠孤病毒的分离、鉴定及生物学特性比较

为研究Ⅱ型草鱼呼肠孤病毒(grass carp reovirus,GCRV)不同分离株毒力强弱及生物学特性的差异,该研究从患病和健康草鱼体内分离到Ⅱ型GCRV各一株,分别命名为ZH180804和CQ180701,并从细胞培养特性、致病性、基因组带型、基因序列差异、遗传进化关系等方面进行比较分析。结果显示,ZH180804对草鱼和稀有鲫的致死率分别为80%和100%, CQ180701对草鱼和稀有鲫的致死率分别为0和10%,初步表明ZH180804为强毒株,CQ180701为弱毒株,且弱毒株感染过的草鱼对强毒株的攻击感染具有较好的免疫保护;2株分离株在草鱼鳔细胞(GSB)、稀有鲫卵细胞(GRE)及稀有鲫尾鳍细胞(GRF)中均能增殖但不产生致细胞病变效应(CPE),且ZH180804株的增殖量是CQ180701株的1 000倍以上;十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)结果显示,2株分离株基因组带型相似,但S7、S8、S9、S10与S11节段的分布存在一定的差异;2株病毒的S7与S11节段的基因序列同源性分别为98%和99%。基于S7与S11节段编码的氨基酸序列构建的...     

Activation of macrophage peroxisome proliferator-activated receptor α inhibits macrophage inflammation-induced cardiac fibroblast activation and migration

AIM: To investigate the effect of macrophage peroxisome proliferator-activated receptor α (PPARα) activation on macrophage inflammation-induced activation and migration of cardiac fibroblasts. METHODS: Mouse bone marrow-derived macrophages were treated with vehicle, PPARα agonist WY14643 (10 μmol/L), angiotensin Ⅱ (Ang II; 1 μmol/L) or Ang II+WY14643 for 24 h, and the supernatants were collected as conditioned medium (CM) to stimulate cardiac fibroblasts for additional 24 h. The mRNA levels of PPARα, interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) in the macrophages as well as fibrotic markers collagen type Ⅰ alpha 2 chain (Col1a2), collagen alpha 1 chain (Col3a1) and actin alpha 2 (Acta2) in the cardiac fibroblasts were detected by RT-qPCR. The protein levels of IL-6 and IL-1β in the macrophages as well as collagen I, collagen III and α-smooth muscle actin (α-SMA; encoded by Acta2 gene) in the cardiac fibroblasts were determined by Western blot. Wound-healing assay was applied to eva-luate the migration ability of cardiac fibroblasts. RESULTS: Ang II significantly increased the mRNA levels of pro-inflammatory factors, such as IL-6, IL-1α and TNF-α, but decreased the mRNA level of PPARα in the macrophages. Administration of PPARα agonist WY14643 dramatically decreased Ang II-induced mRNA levels of IL-6, IL-1β and TNF-α in the macrophages, and significantly decreased Ang II-induced protein expression of IL-6 and pro-IL-1β in the macrophages. The CM from Ang II-treated macrophages significantly up-regulated the mRNA levels of Col1a2, Col3a1 and Acta2 in the cardiac fibroblasts, which were inhibited by the CM from WY14643-treated macrophages. The same results were observed in the protein levels of collagen I, collagen III and α-SMA in the cardiac fibroblasts. Moreover, the CM from Ang II-treated macrophages significantly promoted cardiac fibroblast migration, whereas the CM from WY14643-treated macrophages markedly inhibited macrophage inflammation-induced cardiac fibroblast migration. CONCLUSION: WY14643-activated PPARα inhibits activation and migration of cardiac fibroblasts by attenuating Ang II-induced macrophage inflammatory response.     

Expression profiles of CaMKⅡδ at different stages of osteoclast differentiation

AIM: To study the expression profiles and the role of Ca²⁺/calmodulin-dependent kinase Ⅱ delta (CaMKⅡδ) during osteoclast differentiation.METHODS: Mouse RAW264.7 cells were induced by receptor activator of nuclear factor κB ligand (RANKL) at 50 μg/L for osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption lacunae examination were performed to verify osteoclast formation. The expression of CaMKⅡδ at mRNA and protein levels was also determined by immunofluorescent cytochemistry, RT-qPCR and Western blot at days 0, 1, 3 and 5.RESULTS: TRAP positive multinuclear cells with bone resorption function were formed after 5 d of induction. The mRNA levels of CaMKⅡδ detected by RT-qPCR were 1.028±0.041, 2.478±0.087, 10.524±1.284 and 42.914±2.667 at days 0, 1, 3 and 5, respectively, while the protein levels of CaMKⅡδ detected by Western blot were 0.762, 0.963, 1.802 and 3.136, respectively. The changes of protein level were also verified by immunofluorescence cytochemistry, in which the fluorescence intensity increased in a time-dependent manner (P<0.05).CONCLUSION: The expression of CaMKⅡδ increases with the differentiation of osteoclasts. CaMKⅡδ may play a key role in the osteoclastogenesis.     

张栻的“性善”论说

国内关于内部评级模型中长期中心违约趋势（以下简称“CT”）估计的研究基本处于空白状态，国外文献中也鲜见类似研究。作为新资本协议内部评级模型中的唯一校准参数，商业银行应在监管的审慎性要求和银行自身的准确性要求之间权衡，基于实际违约数据对CT进行合理的估计。为此，在列明监管合规关注点的基础上，构建CT估值框架，设计估值模型，并进行实证研究。压力情境下的实际违约率估算结果表明，CT结果满足了审慎性要求。     

“纱网稻”模式下优质稻品种与有机肥用量二因素试验研究

为完善大棚西瓜-水稻轮作模式“纱网稻”有机栽培技术，2021年在覆盖防虫网条件下，进行品种与有机肥用量二因素试验，品种因素设8个水平、有机肥用量因素设3个水平。结果表明，所有品种在有机肥用量为130kg/667m2时产量最高，筛选出适宜该模式栽培且综合表现较好的两个新品种源稻19(籼稻)、旺两优98丝苗。     

NDV基因Ⅶ型与基因Ⅱ型感染CEF后microRNA表达谱分析

本研究旨在探究新城疫病毒（Newcastle disease virus,NDV）流行株HB株（基因Ⅶ型）与疫苗株La Sota株（基因Ⅱ型）在感染鸡胚成纤维细胞（Chicken embryo fibroblasts,CEF）36 h后宿主细胞microRNA(miRNA)表达谱变化情况。通过高通量测序技术分别对La Sota株与HB株感染CEF后36 h后的样本与参考鸡源基因组进行系统比对，筛选差异表达miRNA并对其靶基因进行GO功能注释以及KEGG通路富集。最后随机筛选5条miRNA通过实时荧光定量PCR(Real-time quantification PCR,RT-qPCR)进行验证。结果表明，La Sota与HB感染组中已知miRNA分别为56.9%和47.8%;La Sota与HB感染组通过差异性比较后共筛选出10条差异表达miRNA；通过对差异表达基因的靶基因进行GO与KEGG富集发现，其主要在细胞代谢、生物合成以及应激信号传导等通路上出现显著富集；RT-qPCR结果与RNA-seq结果趋势一致，证明了测序结果的可靠性。为进一步揭示不同NDV毒株感染CEF以及在感染过程中miRNA发挥的调控作用提供了研究基础。