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51.
将Ⅳ索12型索道应用于狭长工作面的采石场的石材搬运和装车作业。该索道系统中闽林721绞盘机的摩擦卷筒、跑车及游动滑车都进行了改装设计使其达到4倍增力起重、双向牵引,最大起重量可达9t。实践表明在特殊困难地形将林业索道用于采石场作业有明显效益。 相似文献
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氨基酸是蛋白质的基本组成单位,是人体必需的重要营养物质。彩色小麦被认为是一类新型的营养保健小麦品种,但对其籽粒中氨基酸含量的研究很少。本研究首次利用高效液相色谱法比较分析了种植于三个环境中的彩色小麦近等基因系(蓝粒小麦科兴611,紫粒小麦科兴617和白粒小麦轮回亲本济麦22)籽粒中多种氨基酸的含量。结果表明,蓝粒小麦科兴611的必需氨基酸含量、非必需氨基酸含量及总氨基酸含量均显著地高于紫粒小麦科兴617和白粒亲本济麦22。紫粒小麦科兴617和白粒小麦济麦22相比,必需氨基酸含量、非必需氨基酸含量及总氨基酸含量相近,甚至低于济麦22。因此,蓝粒小麦籽粒氨基酸含量高的性状,有益于满足人类营养需求,同时可作为育种材料改良小麦品种的氨基酸含量,尤其是提高限制性氨基酸的含量,进而提高小麦的营养品质。 相似文献
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AIM:To study the role of ghrelin in cell protection by up-regulating heat shock protein 70 (HSP70) and inhibiting apoptosis induced by oxidative stress through extracellular regulated protein kinases 1/2 (ERK1/2) signaling pathway in the PC12 cells. METHODS:Sodium nitoprusside (SNP) was used to induce oxidative stress injury in the PC12 cells. The cultured PC12 cells were divided into SNP-injured group (incubated with SNP at 0.5 mmol/L for 6, 12, 18 and 24 h), ghrelin pretreatment group (ghrelin at 100 nmol/L was given 30 min before adding SNP); HSP70 inhibitor group (quercetin at 10 μmol/L was added 60 min before ghrelin treatment), ERK inhibitor group (ERK 1/2 inhibitor PD98059 was added 60 min before ghrelin treatment) and control group (added same amount of culture medium only). The apoptotic rate was detected by flow cytometry. The protein expression was determined by Western blot and immunocytochemistry. RESULTS:Compared with control group, the apoptotic rate of PC12 cells in SNP-injured group was significantly increased (P<0.05). Compared with SNP-injured group, ghrelin (100 nmol/L) pretreatment significantly inhibited SNP-induced apoptosis of PC12 cells (P<0.05), and significantly up-regulated the protein expression of HSP70 (P<0.05). Time-effect analysis showed that ghrelin had the most significant effect at 18 h after SNP injury. Quercetin, an inhibitor of HSP 70, significantly reduced the anti-apoptotic effect of ghrelin (P<0.05). Ghrelin pretreatment promoted the phosphorylation of ERK1/2. ERK1/2 inhibitor PD98059 significantly inhibited the effects of ghrelin on up-regulation of HSP70 expression (P<0.05). CONCLUSION:Ghrelin upregulates the expression of HSP70 and inhibits the apoptosis in the PC12 cells induced by oxidative stress by promoting the phosphorylation of ERK1/2. 相似文献
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通过比较野生型菌株Wt01-23与StSte12基因RNAi沉默突变体菌株StRNAi9-10和StRNAi3-6在H_2O_2胁迫下生长和发育方面的差异,分析转录因子基因StSte12对玉米大斑病菌氧化胁迫的调节能力。在不同浓度H_2O_2胁迫条件下,测定野生型菌株和突变体菌株的菌落生长速度、菌丝形态、产孢量和菌丝萌发率。结果表明,随着H_2O_2浓度的增加,玉米大斑病菌野生型菌株和突变体菌株的菌落生长速度、产孢量和菌丝萌发率均显著降低,但突变体菌株的降低程度显著高于野生型菌株,表明StSte12基因对玉米大斑病菌的氧化应激调节具有重要的调控功能。 相似文献
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XIA Zi-rong LI Qing XIA Zhen LI Ju-xiang HONG Kui WU Yan-qing WU Qin-hua CHENG Xiao-shu 《园艺学报》2017,33(12):2238-2244
AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G0/G1-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis. 相似文献
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“高粱+再生高粱”高产高效栽培技术研究 总被引:2,自引:0,他引:2
为提高高粱在"种植一季有余、两季不足"的温光资源条件下的种植效益,以‘泸糯12号’为研究材料,通过优化播期、促芽肥等关键技术研制"高粱+再生高粱"高产高效栽培技术,再与传统技术进行对比试验。结果表明,"高粱+再生高粱"高产高效栽培技术要点是2月底至3月5日前播种,种植密度101055株/hm~2,头季高粱收获前10天及时施纯氮150 kg/hm~2,留桩高度控制在近地面第1节位,每窝保留再生苗2~3苗。应用"高粱+再生高粱"高产高效栽培技术后两季高粱产量和效益,较传统高粱种植技术分别增长120.3%、84.3%,增产增效效果显著。因此,"高粱+再生高粱"高产高效栽培技术适宜区域大面积推广。 相似文献
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