响应面法优化龙牡宁神汤中君臣药的提取工艺

以龙牡宁神汤组方为原料,选取影响中药提取率的五个因素,即料液比、浸泡时间、提取时间、提取温度、提取次数进行单因素试验,在此基础上选取三个因素三个水平,以中药浸膏得率为响应值,进行响应面分析,优化其君臣药水提工艺,为工业化生产,开发新剂型提供理论依据;本研究实际提取率与理论值恰合良好,确定的最佳提取条件：浸泡时间为45min,料液比为1：15,提取时间为50 min,提取温度为100℃,反复提取2次,首次提取率为8.15%,操作简单易行,为中药开发奠定了基础。     

滋肾活血汤治疗子宫内膜薄致月经过少肾虚血瘀证的疗效观察

目的观察滋肾活血汤治疗子宫内膜薄致月经过少肾虚血瘀证的临床疗效。方法选择2012年9月—2013年9月期间在我院妇科门诊治疗的子宫内膜薄致月经过少肾虚血瘀证60例患者,随机分为观察组与对照组各30例。观察组口服滋肾活血汤,对照组口服补佳乐联合黄体酮,3个月为1疗程。观察两组临床疗效、治疗前后中医症状评分、子宫内膜厚度变化。结果观察组治疗后临床疗效和中医症状评分优于对照组(P<0.05)。两组治疗后子宫内膜厚度与本组治疗前比较,均有明显改善(P<0.05),两组治疗后子宫内膜厚度比较差异无统计学意义(P>0.05)。结论滋肾活血汤治疗子宫内膜薄致月经过少肾虚血瘀证具有显著疗效,且能增加子宫内膜厚度,值得临床推广应用。     

补肾化癖方对PCOS大鼠卵巢颗粒细胞 CYP450表达的影响

〔摘要〕目的观察补肾化疲方对多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞CYP450蛋白表达的影响〔方法 运用补肾化疲方对PCOS模型大鼠进行干预后,采用免疫组化法观察卵巢颗粒细胞CYP450的蛋白表达〔结果模 型组与正常组比较,卵巢颗粒细胞CYP450的蛋白表达在表达面积及表达强度上均低于正常组,差异具有统计学 意义(P<0.01);补肾化疲方中、高、低剂量组及妈富降组用药后,卵巢颗粒细胞的CYP450的蛋白表达明显升高,差 异具有统计学意义(P<0.01, P<0.05);其中补肾化疲方中剂量对PCOS大鼠卵巢颗粒细胞CYP450的蛋白表达效果 最佳、结论补肾化疲方能增强PCOS大鼠卵巢颗粒细胞CYP450的蛋白表达,调节卵巢内分泌失调,改善PCOS高 雄血症.逆转卵泡的发育异常.使排卵成功、     

长白山人参对小鼠的急性毒性实验

目的 观察人参水煎液对小鼠灌胃给药后急性毒性反应,评价其安全性,为中医临床应用提供参考依据.方法:以人参水煎液最大浓度、最大体积对小鼠进行一次灌胃给药,观察7d内小鼠的毒性反应以及死亡情况.结果 人参水煎液2g/ml,0.4ml/10g进行灌胃给药,与成人日用人参量3～9g相比,相当于成人临床用量的266.67～800倍,却未见药物引起的毒性反应和死亡情况.结论 人参水煎液具有较好的安全性.     

针药结合治疗风痰阻络证中风后吞咽障碍患者75例临床观察

目的 观察化痰通咽汤结合针灸治疗中风后风痰瘀阻证吞咽障碍的临床疗效。方法 选取在武汉市第一医院康复医学科住院的证属风痰瘀阻证中风后吞咽障碍患者150例,随机分为对照组和治疗组各75例。治疗组采用针刺结合化痰通咽汤进行治疗,对照组采用针刺治疗,两组均给予神经内科基本治疗和康复治疗,疗程4周,对比临床疗效。结果 治疗后两组洼田饮水试验评分,吞咽功能障碍中医评价量表评分,电视X线透视吞咽功能检查,吞咽困难生活质量测量比较差异均有统计学意义,治疗组优于对照组（P<0.05）。结论 在神经内科基本治疗和康复治疗基础上综合运用化痰通咽汤结合针灸治疗中风后风痰瘀阻证吞咽障碍患者,能提高疗效,值得推广应用。     

白头翁汤治疗鸡球虫病的疗效观察

对3种不同浓度的白头翁汤治疗鸡球虫病进行了疗效和病理组织学观察.结果表明,中药Ⅳ组(1.5%白头翁汤)抗球虫指数(ACI)为177.35,比中药Ⅲ组(2.0%白头翁汤)、中药V组(1.0%白头翁汤)的抗球虫指数(134.17、107.91)更有效.中药组具有较强的抗球虫作用,存活鸡的病理组织学观察显示,中药Ⅳ组肠管、肺脏里有淋巴细胞增生,中药Ⅲ组肝脏、心肌有轻度的变性.     

Combined effect of octreotide and Dachaihu decoction on treatment of severe acute pancreatitis

AIM: To investigate the combined effect of octreotide and Dachaihu decoction on the treatment of severe acute pancreatitis(SAP). METHODS: Wistar rats(n=50) were randomly divided into sham group, SAP group, octreotide group, Dachaihu decoction group and combination group. The quantity of ascites was measured. The levels of amylase, alanine aminotransferase and creatinine in the serum were examined. The morphological changes of the pancreatic tissues were observed by HE staining. The activation of NF-κB and IκBα expression were determined by Western blot. The mRNA expression with ICAM-1 and IL-1 was detected by qPCR.RESULTS: Combined treatment with octreotide and Dachaihu decoction effectively reduced the quantity of ascites and the levels of amylase, alanine aminotransferase and creatinine in the serum in SAP rats. Moreover, combined treatment significantly inhibited SAP-induced activation of NF-κB and decrease in IκBα protein expression, accompanied by a decrease in ICAM-1 and IL-1 mRNA expression. CONCLUSION: Combination of octreotide with Dachaihu decoction effectively attenuates SAP by inhibiting NF-κB signaling pathway and ICAM-1 and IL-1 expression.     

Effect of Sini decoction on expression of caveolin-1 and eNOS in EAhy926 cells injured by homocysteine

AIM：To investigate the mechanism of Sini decoction in treating human vascular endothelial cell injury and the roles of caveolin-1 and nitric oxide (NO) system in this procedure. METHODS：Model of human umbilical vein endothelial EAhy926 cells injured by homocysteine (Hcy) was established. The protective effect of Sini decoction on the injured EAhy926 cells was observed, and the expression of caveolin-1 and endothelial nitric oxide synthase (eNOS) was detected by real-time fluorescence quantitative PCR and Western blotting. RESULTS：Compared with control group, the Hcy-treated EAhy926 cells showed reduced adherent cell number and NO concentration in culture supernatant, decreased expression of eNOS mRNA and protein, and increased expression of caveolin-1 mRNA and protein (all P<0.05). Compared with Hcy group, better growth of adherent cells, elevated NO concentration in culture supernatant, attenuated expression of caveolin-1 mRNA and protein, and enhanced expression of eNOS mRNA and protein in Sini decoction groups were observed (all P<0.05). CONCLUSION：Homocysteine may injure EAhy926 cells by enhancing the expression of caveolin-1 and suppressing the expression of eNOS, while Sini decoction may protect EAhy926 cells by suppressing the expression of caveolin-1 and enhancing the expression of eNOS.     

黄连解毒汤加味对脓毒症热毒炽盛证患者血流动力学和微循环灌注的影响

目的 探讨黄连解毒汤加味对脓毒症热毒炽盛证患者血流动力学及微循环灌注的影响。方法 选取符合诊断标准的患者60例,按随机数字表法将患者分为观察组30例及对照组30例,对照组患者采用传统抗感染、对症支持、稳定内环境等西医常规治疗,观察组患者在对照组的基础上加用黄连解毒汤加味治疗。观察两组患者治疗前及治疗后24 h、48 h血流动力学及微循环灌注指标的变化。结果 治疗24 h、48 h后,两组患者心率（heart rate, HR）、平均动脉压（mean arterial pressure, MAP）、中心静脉压（central venous pressure, CVP）、体循环阻力（systemic vascular resistance, SVR）、血管活性药用量（norepinephrine, NE）、血乳酸（blood lactic acid, LAC）、混合静脉血血氧饱和度（oxygen saturation of mixed venose blood, SCVO₂）、动静脉二氧化碳分压差（significance of venoarterial carbon dioxide difference, Pcv-aCO₂）均较治疗前明显改善（P<0.05）,且观察组各项指标较对照组改善明显（P<0.05）。结论 对脓毒症热毒炽盛证患者联合应用西医常规配合黄连解毒汤加味治疗,可以有效改善患者血流动力学指标及组织低灌注水平,为临床治疗提供参考。     

补肾活血方对PC 0小鼠未成熟卵母细胞 体外核成熟的影响

〔摘要〕目的探讨补肾活血方对PCO小鼠未成熟卵母细胞体外核成熟的影响、方法(1)制备补肾活血方含药 血清;}2)制作PCO小鼠模型;}3)将PCO小鼠生发泡(germinal vesicle , GV)期卵母细胞分别在不同采血时间获取的 补肾活血方含药血清培养液中进行体外培养,观察补肾活血方含药血清对生发泡破裂(germinal vesicle break- down,GVBD)和第一极体(the first polar body,PBl)排出的时效关系结果(1)2 h和2.5 h药物血清组卵母细胞 GVBD的发生率高于正常血清组和对照组,培养后4 h差异有统计学意义(P<0.01) ; (2)2 h和2.5 h药物血清组卵 母细胞PB1的发生率高于正常血清组和对照组,培养后18 h差异有统计学意义(P<0.01)、结论2-2.5 h补肾活血 方含药血清对PCO小鼠未成熟卵母细胞体外核成熟具有明显促进作用、