Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.     

2013～2014年我国进境货物疫情分析与建议

本文对2013~2014年我国进境货物检疫截获情况进行了分析,发现检疫截获昆虫较多、其他有害生物相对较少,有害生物鉴定等级较低,首次截获有害生物风险评估与检疫处理制度不完善以及某些国家(如巴西、阿根廷、所罗门群岛、喀麦隆)批次截获率明显高于其他国家等情况,并提出了合理化建议,以期对未来植物检疫工作提供参考。     

氟唑菌苯胺原药高效液相色谱分析方法研究

本文采用高效液相色谱法,以乙腈+磷酸溶液为流动相,使用ZORBAX SB-C18色谱柱和二极管阵列检测器,在240nm波长下对试样进行分离和定量分析。结果表明,该分析方法的线性相关系数为1.000 0,标准偏差为0.15,变异系数为0.15%,平均回收率为100.04%。     

Prevalence,molecular characterization and risk factor analysis of Ehrlichia canis and Anaplasma platys in domestic dogs from Paraguay

This is the first study to investigate the prevalence and risk factors associated with Ehrlichia canis and Anaplasma platys positivity in dogs from Paraguay. Conventional PCR assays for the E. canis 16SrRNA gene and A. platys p44 gene were carried out in blood samples from 384 dogs from Asunción city, Paraguay. Sequencing and phylogenetic analysis were performed in selected positive E. canis and (16SrRNA gene) and A. platys (16S and p44 genes) samples. The overall prevalence of E. canis and A. platys in dogs in Paraguay was 10.41% (40/384) and 10.67% (41/384), respectively. Older dogs without veterinary care had higher odds for E. canis positivity and a higher number of dogs in the same household, as well as absence of anti-tick treatment were considered risk factors for A. platys. Ehrlichia canis and A. platys circulate in the dog population from Asunción, and are described for the first time in Paraguay.     

河西荒漠灌区紫花苜蓿施肥效应及其基于数据包络分析法的经济效益研究

为科学准确地评判河西走廊苜蓿主产区紫花苜蓿饲草生产中施肥措施对其产品质量及生产收益的影响,本研究以“甘农3号”紫花苜蓿为材料,通过2016、2017年2年田间试验,以该区域紫花苜蓿饲草生产的平衡施肥推荐方案(N 103.5 kg·hm^-2、P₂O₅ 105 kg·hm^-2、K₂O 90 kg·hm^-2)为对照,探讨了不施肥及3种不完全施肥(缺氮偏施、缺磷偏施、缺钾偏施)处理下紫花苜蓿的生产性能,并采用数据包络分析法(DEA)对其经济效益进行分析。结果表明:与不施肥相比,施肥措施各处理均显著提高紫花苜蓿产量、蛋白总量,降低其酸性洗涤纤维和中性洗涤纤维,提高了相对饲用价值,从而改善了紫花苜蓿品质,并有效增加了经济效益;与氮、磷、钾平衡施肥相比,各偏肥处理的紫花苜蓿产量和品质均显著低于平衡施肥,尤以缺磷偏施的降幅最大,2016、2017年2年的产量和蛋白总量降幅分别达到25.9%、25.7%和33.4%、33.1%。因此,磷是河西荒漠灌区紫花苜蓿饲草生产的养分限制因子,氮、磷、钾对该地区紫花苜蓿生产性能影响顺序为:磷>氮>钾。运用数据包络分析法(DEA)分析出河西荒漠灌区紫花苜蓿的施肥效应为氮、磷、钾平衡施肥的经济效益最优,为DEA有效;不完全施肥的3个评价单元及不施肥评价单元为DEA无效,其中,不施肥经济效益最低,3个不完全施肥评价单元中的缺磷偏肥的紫花苜蓿经济效益比缺氮偏肥和缺钾偏肥更低;另外还以DEA模型推算出不同施肥措施下紫花苜蓿饲草生产经济效益改进的具体方案,其中,不施肥的紫花苜蓿饲草生产需调整的幅度最大,调整额度达10678.88 CNY·hm^-2,各施肥措施需调整的幅度排序为:不施肥>缺磷偏施>缺氮偏施>缺钾偏施。     

天然更新伯乐树的生长过程初步分析

解析1株天然林中148年生伯乐树倒木树干，表明其树高连年生长量在65年生时达到最大值，和树高年平均生长量曲线相交于80年生时；胸径连年生长量在70年生时达到最大值，与胸径年平均生长量相交于95-100年生时；而材积连年生长量的最大值出现在80年生时，与材积年平均生长量于135-140年间相交，此时可以认为是伯乐树的材积数量成熟年龄。     

奶山羊CIDEa基因的克隆、序列分析与组织表达

为获得奶山羊CIDEa基因序列并检测其在乳腺组织中的表达,采用RT-PCR技术从奶山羊乳腺组织扩增CIDEa基因序列,利用生物信息学软件对其进行预测分析,并采用荧光定量PCR检测CIDEa在干奶期和不同泌乳时期乳腺组织中的表达。结果表明:通过克隆测序得到奶山羊CIDEa基因CDS区660 bp,编码219个氨基酸,与GenBank公布的绵羊、牛、人和猪的核苷酸序列同源性分别为99%、96%、85%、85%。奶山羊CIDEa蛋白属于碱性、不稳定亲水蛋白,不存在跨膜结构和信号肽;蛋白主要定位在细胞质、线粒体和细胞核。CIDEa蛋白结构主要由α螺旋、β折叠和不规则卷曲组成。奶山羊CIDEa在泌乳前期、盛期和中期乳腺组织中表达量均显著高于干奶期(P<0.05)。说明CIDEa可能参与奶山羊乳腺的泌乳过程,为进一步研究其在奶山羊乳腺组织中的功能及对乳品质的调控作用奠定基础。     

灯盏花成分专用型种源评价与鉴定

收集和鉴定了云南省和贵州省共46份灯盏花种质资源,筛选出适合生产不同药品的成分专用型种源,为专用型品种选育提供参考。利用HPLC测定了灯盏花种源10种活性成分含量,相关分析阐明各成分含量间的相关性,聚类分析对种质资源进行分类。结果表明：16份灯盏花种源灯盏乙素（scutellarin, SE）含量在2.5%以上,是培育灯盏花素专用型品种的优良材料,其中4份种源同时含有高含量（>1.0%）的双咖啡酰奎宁酸酯（dicaffeoylquinic acids, diCQAs）,是培育灯盏细辛专用型品种的优良材料;同时,筛选出8份总黄酮（total flavonoids, TFs）含量高于3.5%的种源,适合选育总黄酮专用品种。相关性分析表明,SE含量与diCQAs和TFs含量均显著正相关,是灯盏花品质育种的标志性成分。聚类分析将灯盏花种源分为三大类,除1个种源的SE含量和diCQAs含量接近（第Ⅲ类）外,其他种源SE均占总活性成分的60%以上,其中第Ⅰ类为高TFs型种源,TFs占到总活性成分的80%以上,diCQAs占总成分的15%以下;第Ⅱ类为高diCQAs型种源,diCQAs占总成分的16.9%~28.9%,TFs占到总活性成分的80%以下。该结果表明灯盏花种源活性成分组成存在多种类型,为专用型品种选育提供了可能,灯盏乙素含量可以作为专用型种源筛选的首要指标。