基于TPA模式对3个葡萄品种果实质构性能的比较

为了探讨不同葡萄品种果实质地的多面分析方法并筛选适宜的评价指标,以我国3个主栽葡萄品种巨峰、玫瑰香、红地球的成熟果实为试材,采用质地多面分析(TPA)法,利用物性测试仪测定了果肉的硬度、弹性、凝聚性和咀嚼性;并利用SPSS数据分析软件,对葡萄果肉4个质地参数之间的相关性进行了分析。结果表明:红地球的果肉硬度、弹性、凝聚性和咀嚼性均显著高于其他2个品种,而玫瑰香与巨峰的果肉质地参数除弹性外其他指标差异均不显著;质地参数两两之间均呈正相关,其中,咀嚼性与硬度的相关性达到了显著水平(P0.05)。在对葡萄果肉质地进行简单评价时,可以将去皮硬度作为反映葡萄咀嚼性的重要指标。     

山西乡宁地区2个红色酿酒葡萄果实生长发育过程中几类多酚物质变化分析

果实酚类物质质量分数及其组分是酿造优质葡萄酒的重要基础。以山西乡宁地区的‘赤霞珠’(Cabernet Sauvignon)、‘梅鹿辄’(Merlot)为供试品种,从盛花后到果实成熟连续采样分析果皮和种子内酚类物质的变化规律,为实施优质酿酒葡萄栽培技术奠定基础。结果表明:葡萄果实在发育过程中呈现单"S"曲线;种子与果皮内总酚、单宁及总类黄酮质量分数均逐渐下降,但在采收前略有升高,种子内质量分数明显高于果皮内;在果实成熟过程中,果皮内花色苷总量呈现先上升后下降的趋势;不同品种之间,酚类物质变化趋势有较大差异。     

采前因素对葡萄贮藏质量的影响

针对我国鲜食葡萄产量高,贮藏量相对较小,贮藏损失严重的问题,简述了葡萄品种、气候条件、树体管理、肥水管理等栽培和管理技术以及采收环节等采前因素对葡萄贮藏质量的影响。     

葡萄果实cDNA文库的构建及鉴定

构建葡萄果实cDNA文库是研究果实发育相关基因表达调控的基础工作.本实验从巨峰葡萄果实中提取了高质量的RNA,利用MMLV反转录酶把总RNA中的mRNA反转录成cDNA第一链,用特异引物进行LD-PCR扩增合成双链cDNA.将分级纯化的带有粘性末端双链cDNA与λTrip Ⅰ Ex2载体连接,重组载体体外包埋成为cDNA原始文库.初步鉴定原始文库的滴度为1.32×106 pfu/mL,文库扩增后滴度达1.45×1010 pfu/mL.从扩增文库中随机挑取250个克隆进行PCR鉴定,结果表明重组率为98.7%,插入片段大小在0.5～2.0 kb之间,因此,本研究成功构建了葡萄果实cDNA文库.     

2例犬皮肤肥大细胞瘤细胞学与病理组织学观察

为了确定细胞学检查在犬皮肤肥大细胞瘤诊断中的诊断意义,采取细胞学检查和病理组织学检查相结合的方法对2例犬皮肤肥大细胞瘤临床病例进行诊断.结果显示:细胞学检查结果与病理组织学检查结果相符.由此可以得出,在犬皮肤肥大细胞瘤的诊断中,细胞学检查具有一定的诊断价值,为肿瘤的性质及分化程度分级提供参考,为该病临床诊断提供了一种新...     

鹿茸发育的组织来源及其相互作用机制研究进展

本综述介绍了鹿茸发育的组织来源及其相互作用机制的研究进展,阐明了鹿茸发育的组织细胞特性,进一步分析了鹿茸发育机制中待解决的问题。     

不同架式对设施葡萄光合特性及果实品质的影响

以设施红提葡萄为试材,研究不同的整形方式对葡萄叶片光合特性和光合关键酶活性的影响。结果表明:与扇形整形方式相比,L形和FI形整形方式可明显提高叶面积;在果实迅速发育时期,L形整形方式显著提高了叶绿素含量,Rubisco及Rubisco活化酶活性显著升高;同时,叶片质膜ATPase和叶绿体膜ATPase活性也显著升高,从而提高了叶片的光合速率。L形、FI形和V形整形方式均不同程度提高了平均单果质量、可溶性固形物含量,降低了可滴定酸含量,而L形则显著地提高了果实的品质。因此,适当的整形方式可通过调节提高设施葡萄的叶绿含量、光合关键酶和ATPase活性来提高光合速率,最终提高果实的品质。     

Dermatophilus congolensis Infection in Goats in Tanzania

When goats in Eastern Tanzania were screened for skin diseases, Dermatophilus congolensis was isolated from the skin lesions in 8 of 484 animals examined. In one severely affected case, the disease was also characterized by histological studies (Gram stain, Giemsa stain and routine HE studies) and electron microscopy. The histological picture was characterized by hyperkeratosis, parakeratosis, acanthosis, folliculitis and an inflammatory cellular reaction involving the epidermis. Gram stain and Giemsa stain revealed longitudinal and transverse branching filaments in the deeper layers of the epidermis. Ultrastructural studies demonstrated D. congolensis in various morphological forms, ranging from filamentous to tuber-shaped structures, mixed with numerous coccoid bodies of variable size. In some instances, the organisms were geometrically arranged in parallel rows of beading and were present in and among the degenerated epithelial cells. Several host cells showed degenerative changes. Ticks present on the goats were Amblyomma variegatum, Rhipicephalus evertsi, Rhipicephalus pravus and Boophilus sp. The clinical signs, pathological lesions, diagnosis, epidemiology and pathogenesis of the disease are discussed.     

Spongiotic vesicular dermatitis as a cutaneous reaction pattern in seven horses

Over a 6-year period seven adult horses of different breeds and genders developed multifocal, exudative, oozing dermatitis characterized histologically by epidermal spongiotic vesicles and perivascular eosinophilic, neutrophilic and mixed mononuclear inflammation. Three horses were pruritic. Systemic disease was not noted. Two horses had a history of recurrent urticaria (hives) and one horse had nodules or welt-type lesions that progressed to exudative, oozing lesions. Interepithelial immunoglobulin (Ig)G was detected by avidin-biotin complex-peroxidase staining, but the pattern of staining was more consistent with epithelial oedema than specific IgG deposition associated with pemphigus. The exudative oozing lesions developed under circumstances suggesting that dermal oedema progressed to intracellular and intercellular epidermal oedema, which in turn progressed to the spongiotic vesicular epidermal lesions.     

The histological lesions of Trichophyton mentagrophytes var erinacei infection in dogs

A retrospective study of the histological features of four cases of canine Trichophyton menta-grophytes var erinacei infection is reported. In all four dogs the initial lesions affected the dorsal muzzle and in two dogs the lesions spread to more distant sites on the body. Clinically, the lesions were characterized by scaling, crusting and hair loss. Histologically, the main lesions were characterized by acanthosis, epidermal, ostial and infundibular hyperkeratosis, serocellular crusting, mural folliculitis and furunculosis. Fungal hyphae were usually sparse and often difficult to see in haematoxylin and eosin stained sections. When visible they were seen in the epidermal, ostial and infundibular scale and, less frequently, within hair shafts.