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41.
Siratro (Macroptilium atropurpureum (DC) Urb.) is a vigorous perennial forage legume with good potential for improving pastures in the extensive neotropical regions of the world. It is well adapted to a wide range of soil and climatic conditions. The objective of these studies was to determine effects of Glomus fasciculatum colonization, rigorous defoliation, and soil fertility treatments to a Psammentic Paleustalf (Eufaula) soil on growth, regrowth, nodulation, and nitrogenase activity (C2H2 red.) of Siratro inoculated with Rhizobium leguminosarum Frank. Top growth increased significantly with soil K and P amendment and with mycorrhiza colonization. Nodulation and nitrogenase activity were correlated with highly significant increases from G. fasciculatum, P treatments and K additions to 300 mg K kg‐1 soil. Growth and peduncles of nonclipped plants increased about 4 fold from 90 to 225 day age with mature seed yield increasing about 10 fold; nodule mass and nitrogenase activity levels approximately doubled. Regrowth response of plants defoliated at 45 day intervals, following their initial 90 day age, was somewhat constant between clippings for magnitude of regrowth 12.3–13.8g, development in number of peduncles 4.0–6.8, seed yield 1.4–2.6g, nodulation 2.9–3.7g, and nitrogenase activity 73.9–95.8μ mol C2H4g‐1 nodule. Multiple regression for nitrogenase = 0.55 g top wt. + 0.63 g nodule wt. + 1.91 day age ‐ 0.07 peduncle no., R2 = 0.85 and C.V. = 14.3%. Favorable tripartite symbiosis with both effective Rhizobium and endophyte mycorrhiza were essential for high levels of symbiotic nitrogen fixation. 相似文献
42.
To compare the effect of substrate-based and commercial arbuscular mycorrhizal fungi (AMF) in salt stress tolerance of Romaine lettuce a bifactorial analysis was carried out. Under non-saline conditions, only plants inoculated with formulation 1 stimulated shoot weight but not related with greater root AMF colonization. Phosphorus and potassium concentrations in leaves were improved by mycorrhizal association. Irrigation with 100 mM sodium chloride (NaCl) did not affect leaf relative water content and we observed no osmotic adjustment in leaves from non-mycorrhizal plants. However, root dry biomass and its starch content decreased, while leaf starch and root soluble sugar concentrations were enhanced. Lettuce inoculated with formulation 2 and substrate-based Glomus intraradices showed the highest root colonization percentages. Nevertheless, none of the mycorrhizal treatments induced a significant improvement on growth of lettuce subjected to salt stress. Romaine lettuce seems to be a moderately tolerant variety to salinity and therefore, the contribution of AMF was minimized. 相似文献
43.
黄芪幼苗丛枝菌根形成过程研究 总被引:1,自引:0,他引:1
盆栽灭菌条件下,研究了黄芪Astragalus membranaceus与摩西球囊霉Glomus mosseae形成丛枝菌根的过程及细胞结构变化情况。结果发现,黄芪接种34 d时,真菌菌丝开始侵染根表皮;44 d时菌丝在皮层细胞内逐渐增多,并且细胞间有极少量泡囊产生,形状为椭圆形;54 d时细胞内大量菌丝扩展;63 d时有大量真菌孢子萌发;84 d时细胞间有大量泡囊形成,呈圆形、近圆形或椭圆形,部分泡囊开始收缩衰老,同时皮层细胞内有少量丛枝并存。生长期间,菌根侵染率和孢子密度的变化与丛枝菌根的发育状况密切相关。 相似文献
44.
P. Mäder Stephan Edenhofer Thomas Boller Andres Wiemken Urs Niggli 《Biology and Fertility of Soils》2000,31(2):150-156
Arbuscular mycorrhizal (AM) root colonization was studied in a long-term field trial in which four farming systems currently
in use in Switzerland were continuously applied to a randomized set of plots at a single field site from 1978 till 1993. There
were two low-input farming systems (organic and bio-dynamic) and two high-input (conventional) farming systems (according
to Swiss guidelines of integrated plant production with and without farmyard manure). The systems had an identical 7-year
crop rotation and tillage scheme and differed essentially only in the amount and type of fertilizer supplied and in plant
protection management. The percentage of root colonization by AM fungi was determined in field samples 2–3 times over the
growing season in crops in the rotation, namely in winter wheat (Triticum aestivum L. cv. Sardona), vetch-rye and grass-clover. We found the percentage of root length colonized by AM fungi to be 30–60% higher
(P≤0.05) in the plants grown in soils from the low-input farming systems than in those grown in conventionally farmed soils.
Approximately 50% of the variation of AM root colonization was explained by chemical properties of the soils (pH, soluble
P and K, exchangeable Mg), the effect of soluble soil P being most pronounced. The potential of the field soils from the differently
managed plots to cause symbiosis with AM fungi was tested in a glasshouse experiment, using wheat as a host plant. Soils from
the low-input farming systems had a greatly enhanced capacity to initiate AM symbiosis. The relative differences in this capacity
remained similar when propagules of the AM fungus Glomus mosseae were experimentally added to the soils, although overall root colonization by AM fungi was 2.8 times higher.
Received: 27 August 1999 相似文献
45.
Summary Concentrations of phenamiphos ranging from 0 to 40 rg/g soil were established in a typical Oxisol (Tropeptic Eutrustox), inoculated or uninoculated with Glomus aggregatum. The effect of the nematicide on the development of vesicular-arbuscular mycorrhizal (VAM) symbiosis was evaluated in the greenhouse using Leucaena leucocephala as an indicator host plant. Treatment of soil with phenamiphos did not have a significant influence on the development of mycorrhizal activity measured in terms of subleaflet phosphorus concentrations. Similarly, the nematicide did not have an adverse effect on the level of mycorrhizal colonization or on the P content of shoots, as determined at the time of harvest. However, shoot dry weight was adversely influenced by phenamiphos when the chemical was applied to the uninoculated soil at 20 g/g soil or higher, and when it was applied to the inoculated soil at 40 g/g soil. It is concluded that phenamiphos is not likely to influence the growth of Leucaena or its symbiotic association with VAM fungi if the concentrations applied do not exceed levels known to suppress nematodes.Hawaii Institute of Tropical Agriculture and Human Resources Journal series No. 3146 相似文献
46.
The influence of V.A. mycorrhization was studied on two clones of in-vitro micropropagated plantlets ofArgania spinosa, a slow-growing tree of great importance in agroforestry systems in the semi-arid and arid zones of southwestern Morocco. Inoculation increased total shoot length, stem girth and biomass of the plants, when cultivated in controlled conditions with a phosphorus fertilization of 31 mgP·kg–1 added to a substrate already containing 13.5 mg·kg–1 available P (Olsen). The positive effect increased with time and, after 6 months, the above-ground dry matter production was 5 to 6 times higher for inoculated plants. The Relative Mycorrhizal Dependency Index, calculated on total dry matter, reached 77–78% at the end of experiment. Calculated on above-ground dry matter, the R.M.D.I. was 81–83%, placing the argan tree among the plants most dependent on mycorrhizal symbiosis. The root/shoot ratio was markedly (40–50%) reduced by inoculation, demonstrating the higher efficiency of a mycorrhizal root system.
Résumé L'influence de la mycorhization V.A. a été étudiée chez l'arganier (Argania spinosa), arbre à croissance lente très important pour les systèmes agroforestiers des zones arides et semi-arides du Sud-Ouest marocain. Chez les plantules issues de micropropagation in vitro et cultivées en conditions contrôlées sur un substrat contenant 13.5 mgP·kg–1 (Olsen) auquel on rajoute 31 mgP·kg–1 par la fertilisation, l'inoculation accroit la longueur totale des axes, le diamètre au collet et la biomasse des plantes issus de multiplication in vitro. L'effet bénéfique augmente avec le temps et, après 6 mois, la matière sèche des parties aériennes était 5 à 6 fois plus élevée chez les plantes inoculées que chez les témoins non inoculés. L'Indice de Dépendence Mycorhizienne Relative, calculé sur la matière sèche totale, atteint 77–78% à la fin de l'expérience. Calculé sur la matière sèche des seules parties aériennes, l'I.D.M.R. est de 81–83%, situant l'Arganier parmi les plantes les plus dépendantes de la symbiose mycorhizienne. Le rapport racines/parties aériennes est nettement réduit (de 40 à 50%) par l'inoculation, mettant en évidence la meilleure efficacité d'un système racinaire mycorhizé.相似文献
47.
Robert K. Dixon 《New Forests》1988,2(3):203-211
Seedlings of Juglans nigra from three seed sources were grown in fumigated soil without vesicular-arbuscular mycorrhizal (VAM) fungi or inoculated with Gigaspora margarita, Glomus deserticola or Glomus etunicatum. Vesicular-arbuscular mycorrhizal development varied significantly between fungal symbionts within a black walnut source. Glomus deserticola and G. etunicatum produced the highest levels of root colonization in all sources. Significant differences in seedling shoot and root growth were attributed to root colonization by specific VAM fungi in each black walnut source. Glomus deserticola stimulated seedling leaf area and root weight 26 and 52%, respectively, in one seed source. Seedling leaf N, P and K concentrations were significantly improved by VAM in two seed sources. Juglans nigra seedlings respond favorably to VAM colonization. However, differences between seed sources suggest a strong host-symbiont interaction. 相似文献
48.
Rakesh Pandey 《Phytoparasitica》2005,33(3):304-308
The sedentary endoparasitic root-knot nematode [RKN],Meloidogyne incognita (Kofoid and White) Chitwood, is worldwide an economically important agri-pest, reducing the yield and quality of crops. The
traditional method of RKN control is based mainly on chemical nematicides, which will not be frequently available from 2005
onwards; their use is highly objectionable due to their major contribution to ground water contamination, and dangers to the
environment and to human and animal health. Consequently, new and environmentally safe tools for RKN management are urgently
needed. The objective of the present investigation was to evaluate different bio-organic wastes on RKN infestation and the
growth / oil yield ofArtemisia pallens Wall. The tested wastes were: 1. Distillation waste ofMentha arvensis (menthol mint); 2.Murraya koengii (meethi neem / curry leaf); 3.Cymbopogon flexuosus (lemongrass); 4.C. martinii (palmarosa); 5.C. winterianus (citronella); 6.Pelargonium graveolens (geranium); 7.Pogostemon patchouli (patchouli); 8.Tagetes minuta (marigold); 9. Vermicompost ofM. arvensis, Chrysanthemum cinerariaefolium (pyrethrum),Tagetes minuta andC. winterianus; 10.Trichoderma harzianum isolate U; 11. VA fungiGlomus aggregatum; G. fasciculatum. The results obtained with these different bio-organics were compared with three controls: untreated — uninoculated, untreated
— inoculated and the chemical pesticide carbofuran. Results revealed a considerable enhancement of herbage biomass, flower
bud and oil yields when plants were treated with the distillation waste ofC. martinii, C. winterianus, M. koengii, M. arvensis, vermicompost ofC. cinerariaefolium, T. minuta, M. arvensis and bio-agentT. harzianum. Least nematode infections were recorded with the distillation waste ofM. koengii, C. martinii, C. flexuosus, and vermicompost ofT. minuta, C. cinerariaefolium andM. arvensis. The results revealed that these environmentally sound bio-organics could be used for replacement of chemical nematicides.
http://www.phytoparasitica.org posting May 20, 2005.
The work was conducted at CIMAP-CSIR, Lucknow, India. 相似文献
49.
秦岭南坡火地塘林区油松、落叶松林地内生菌根真菌的研究 总被引:5,自引:1,他引:4
对秦岭南坡火地塘林区7种林分进行设点、采土、湿筛倾斜分离鉴定。共分离鉴定出17种内生菌根(VAM),逮属Glom us、Gigaspora、Acaulospora 属,以Glomus为主。不同林分及不同立地条件,内生菌根真菌种类和数量有差别。 相似文献
50.
镉污染环境中丛枝菌根真菌对紫羊茅生长镉积累的影响 总被引:4,自引:0,他引:4
应用盆栽方法模拟不同镉污染土壤状况,研究了Glomus intraradices真菌对紫羊茅生长及吸收积累镉的影响。试验设0、15、50μg-1三个施镉水平,每个施镉水平下分别设接种和不接种两个处理。结果表明,土壤施镉对菌根侵染率无显著影响;施镉量增加显著降低了紫羊茅的生长量;接种Glomus intraradices真菌对紫羊茅地上部的生长无明显影响,但显著降低了根系的生长量而相应增大了冠根比;同时降低了紫羊茅对镉的吸收;通过菌根结构强化镉在根系的固持作用,降低了紫羊茅地上部镉的浓度,减少镉向地上部的分配比例,从而改善紫羊茅地上部的品质。 相似文献