全文获取类型
收费全文 | 7968篇 |
免费 | 291篇 |
国内免费 | 674篇 |
专业分类
林业 | 777篇 |
农学 | 1111篇 |
基础科学 | 2009篇 |
731篇 | |
综合类 | 2365篇 |
农作物 | 547篇 |
水产渔业 | 179篇 |
畜牧兽医 | 674篇 |
园艺 | 427篇 |
植物保护 | 113篇 |
出版年
2024年 | 33篇 |
2023年 | 94篇 |
2022年 | 142篇 |
2021年 | 154篇 |
2020年 | 202篇 |
2019年 | 207篇 |
2018年 | 81篇 |
2017年 | 153篇 |
2016年 | 282篇 |
2015年 | 231篇 |
2014年 | 349篇 |
2013年 | 310篇 |
2012年 | 520篇 |
2011年 | 497篇 |
2010年 | 412篇 |
2009年 | 502篇 |
2008年 | 545篇 |
2007年 | 937篇 |
2006年 | 749篇 |
2005年 | 543篇 |
2004年 | 507篇 |
2003年 | 422篇 |
2002年 | 181篇 |
2001年 | 174篇 |
2000年 | 134篇 |
1999年 | 103篇 |
1998年 | 45篇 |
1997年 | 68篇 |
1996年 | 63篇 |
1995年 | 57篇 |
1994年 | 45篇 |
1993年 | 49篇 |
1992年 | 30篇 |
1991年 | 34篇 |
1990年 | 31篇 |
1989年 | 17篇 |
1988年 | 12篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1981年 | 2篇 |
1963年 | 2篇 |
1955年 | 5篇 |
排序方式: 共有8933条查询结果,搜索用时 16 毫秒
51.
52.
JIANG Xun ZENG Yao-ying HE Xian-hui XU Li-hui DI Jing-fang FENG Zheng ZHAO Jing-xian WANG Qing WANG Tong SHI Jian-bo 《园艺学报》2004,20(6):924-928
AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage. 相似文献
53.
Expression of DNA repair gene ERCC1 and its relationship with PAH-DNA adducts in lung cancer tissues
AIM: To investigate the expression of nucleotide excision repair gene ERCC1 and its relationship with PAH (polycyclic aromatic hydrocarbons)-DNA adducts in lung cancer tissues. METHODS: ERCC1 mRNA expression and the PAH-induced DNA adducts were detected in 150 lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The effects of some exposure factors on the expression of ERCC1 gene and the connection between ERCC1 and PAH-DNA adduct was analyzed. RESULTS: Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 1 of 40 (2.5%) normal lung tissues. Smoking may suppress the expression of ERCC1 gene. The level of PAH-DNA adduct was negatively correlated with the expression of ERCC1 gene, the Spearman coefficient was -0.648, P<0.01. CONCLUSION: ERCC1 is an important nucleotide excision repair gene and may participate in the repair of DNA damage, such as PAH-DNA adduct. Low expression of ERCC1 may play an important role in the development of human lung cancer. 相似文献
54.
AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A. 相似文献
55.
56.
为研究GHITM基因在中华鳖(Trionyx sinensis)胚胎发育及生长中的作用,本研究通过RT-PCR和RACE方法获得了中华鳖GHITM全长cDNA序列;采用实时荧光定量PCR对GHITM mRNA组织表达及不同温度孵化胚胎发育过程中的表达特性进行分析。结果显示,中华鳖GHITM cDNA序列长度为2650 bp,开放阅读框为1050 bp,5?非编码区为123 bp,3?非编码区为1477 bp,编码349个氨基酸,编码蛋白的等电点为10.01,分子量为37.12 kDa。GHITM编码氨基酸序列由胞外区、跨膜区和胞内区组成,7个跨膜域组成跨膜区。GHITM编码氨基酸序列的同源性分析显示,中华鳖与锦龟(Chrysemys picta bellii)和绿海龟(Chelonia mydas)同属一个分支,3种鳄鱼构成一个分支,7种鸟类形成一个分支。实时荧光定量检测显示,GHITM基因在肝脏、肌肉和脑垂体中的表达水平较高,显著高于心脏、性腺、肠、肾脏和脾脏组织(P<0.05);50 g左右和500 g左右雄性个体肝脏中的GHITM基因表达量显著高于雌性个体(P<0.05);低温胁迫孵化能显著抑制胚胎肝脏中GHITM基因的表达(P<0.05)。上述研究结果表明,GHITM基因与中华鳖生长和胚胎发育密切相关,其在中华鳖胚胎中的表达受孵化温度调节。本研究为探讨中华鳖胚胎发育和生长提供理论依据。 相似文献
57.
58.
浅谈工程造价的预结算审核 总被引:2,自引:0,他引:2
工程造价的预结算审核是合理确定工程造价的必要程序及重要手段.本文从工程造价中存在的问题,工程造价审核的内容、方法等三个方面论述了工程造价及其预结算的审核工作过程. 相似文献
59.
工程建设项目的管理程序是基本建设单位进行项目管理的关键.本文从项目的前期准备、工程项目建设、工程项目验收三个阶段论述了基本建设单位对工程建设项目的管理程序. 相似文献
60.