~(14)C示踪研究不同小麦品种开花、灌浆期的光合效率及同化物的运转与分配

应用~(14)C示踪技术测定三个籽粒饱满度不同的小麦品种,结果表明:籽粒饱满度与品种的净光合率、光合持续期和同化物在籽粒中的分配率有关;光合作用后30分钟测定,~(14)C同化物的分配,叶片最高,叶鞘其次,麦穗再次,茎中最低;开花期~(14)C同化物在成熟麦穗中的分配,约占引入量的25—32％,灌浆期~(14)C同化物在成熟麦穗中的分配,对籽粒饱满的两个品种,占引入量的50％以上,而籽粒不饱满的品种,分配率还不到30％。     

杉木不同种源叶养分变异的研究

研究表明,不同种源杉木的叶养分含量在树液流动期、芽膨期、旺盛生长期(6月,9月)和封顶期有明显的差异。在供试种源分布区范围内,树液流动期和6月旺盛生长期的叶P含量与纬度成显著负相关;N/P值与纬度成显著正相关。产地≥10℃积温与树液流动期的P,K含量,N/P值及6月旺盛生长期的N/P值都有显著负相关关系。产地日照时数仅与芽膨期的叶Mg,Ca,P养分的正偏相关关系明显(α=0.10).用统一的线性模式较难反映变异的规律。但经主成分(PCA)分析,揭示出南岭山地优良种源区种源叶养分(尤其是P,Mg)含量高于一般产地种源的趋势。     

COMBINED USE OF ULTRASONOGRAPHY AND CONTRAST ENHANCED COMPUTED TOMOGRAPHY TO EVALUATE ACUTE NECROTIZING PANCREATITIS IN TWO DOGS

The imaging findings in two miniature schnauzers with acute necrotizing pancreatitis are described. Both dogs were treated previously for diabetes mellitus and hyperlipidemia. Vomiting, anorexia, and lethargy were observed in both dogs at presentation. Laboratory evaluations supportive of pancreatitis included left shift, abnormally high serum amylase and lipase activities, hypocalcemia, and abnormally high serum activities of liver enzymes. Sonographically, both dogs had diffusely enlarged hypoechoic pancreatic tissue with anechoic foci compatible with necrosis, abscessation, phlegmon, and pseudocysts formation. Contrast-enhanced computed tomography (CT) findings in both dogs were compatible with pancreatic necrosis. Dog 1 was managed medically for 11 days. Follow-up CT scan in this dog disclosed decreased pancreatic size and increased contrast enhancement compatible with partial resolution of pancreatitis.     

甘蓝抗重金属污染的研究

在被重金属污染的土壤中 ,用Ca(NO3)2 拌种处理甘蓝种子 ,进行甘蓝抗重金属污染试验。结果表明 ,Ca(NO3) 2 拌种可以促进甘蓝种子萌发和幼苗生长 ,增强光合速率和抗逆性 ,使甘蓝叶片中Hg ,Cr,Cd含量分别比对照下降 71.9%～ 97.7% ,2 .77%～ 12 .88% ,38.4 6 %。     

李树叶片细菌性穿孔病的发生规律及防治研究

细菌性穿孔病可导致李树叶片大量脱落,树势衰弱,产量降低,品质下降.研究发现,病害的初侵染源来自枝干病斑,生长季节枝、叶、果互相交叉传染.种植感病品种、树势强旺、雨日多、雨量大为该病的流行因素.选用抗病品种、消除初侵染源、加强栽培管理、生长期用药控制是防治该病的有效措施.     

大电流电流源实现方法

提出2种大电流可控电流源方法:对于输出中小电流电流源,对通用的串联式线性调整可控电流源进行改进,降低调整管的温升;对于输出大电流的电流源,采用PWM控制方法,使调整管工作处于开关状态,调整管的功耗很小,温升低.根据上述方法,分别制作1台样机并进行性能测试,实践证明这2种方法是可行的.     

人工林UHF频段电波传播场强预测模型

人工林无线电波传播场强模型是合理设计人工林环境无线电遥测遥控等通信电路的理论依据.该文在分析无线电波传播机理的基础上确定了人工林UHF频段传播损耗的三大组成部分及相应的计算方法,提出了人工林中某接收点处电场强度的预测模型.经过对北京西山人工侧柏林中不同参数的场强测试数据进行分析,校验了所建模型具有很高的精度.      

甘蓝杂种致死材料的鉴定与遗传分析

杂种致死是一种生殖隔离类型,致死材料的鉴定及遗传分析不仅有助于了解杂种致死的遗传机制,还可以为致死基因的定位和克隆奠定基础。利用已知携带杂种致死基因的甘蓝自交系09-211、09-222、10-260和其他自交系配制的杂交组合为试材,鉴定携带致死基因的新材料,并对甘蓝杂种致死性状进行了遗传分析。结果表明:11-204和11-176是2个新的甘蓝致死材料;11-176与09-222及10-260具有遗传等效性,而11-204与09-211具有遗传等效性;甘蓝杂种致死性状是由2个显性基因互补控制的,其中1个致死基因来自09-211或11-204,另1个致死基因来自09-222或10-260或11-176,其遗传模式符合Dobzhansky-Muller(DM)模式。     

大豆吡哆醇生物合成蛋白基因(PDX)的电子克隆和进化分析

电子克隆(In silicocloning)是随着基因组计划和EST计划实施而发展起来的利用生物信息学手段进行基因克隆的新方法。根据物种间同源基因相对保守的特点,以拟南芥(Arabidopsis thaliana)吡哆醇生物合成蛋白cDNA序列为信息探针,对大豆(Glycine max)EST数据库进行同源搜索和序列拼接,获得了1 280 bp长的大豆吡哆醇生物合成蛋白的基因序列(GenBank登陆号为DQ139265)。经过RT-PCR扩增、基因组PCR扩增、分子克隆和序列分析验证,结果表明与电子克隆序列完全一致。该基因具有完整的开放阅读框架(ORF,20~955 bp),编码311个氨基酸。通过与水稻、日本百脉根、烟草、截形苜蓿等物种的吡哆醇生物合成蛋白序列比对,发现该基因具有高度的保守性。表明根据物种间同源基因序列,对跨物种间EST数据库进行同源检索、筛选、拼接,是克隆基因的有效途径。     

The global adaptation of bread wheat at high latitudes

Spring sown bread wheat is grown at high latitudes in Europe, Asia and North America. However, it is not clear what the associations are among environments, particularly in Asia and North America, and whether or not cultivars developed in one region may adapt in another. A yield trial comprised of cultivars developed in northern Kazakhstan, western Siberia, the Canadian Prairies, northern USA, northeastern China and broadly adapted genotypes bred by CIMMYT in Mexico was planted in all the above mentioned environments in 2002–2004. In general, cultivars performed best within the regions they were developed. However, cultivars developed in northern Kazakhstan/western Siberia were the most broadly adapted at high latitudes; they were not significantly different for grain yield from the locally developed cultivars in both China and Canada. Stronger photoperiod response, greater plant height and larger seed weight appeared to be key adaptive features of these materials. At lower latitudes, the Kazakh/Siberian cultivars were significantly lower yielding than all other materials. When low latitude Mexican sites were removed from the analysis, the Chinese locations tended to associate, whereas most Canadian and Kazak/Siberian locations were negatively associated with those from China. SSR analysis of the cultivars from each region split the materials into two general groups, one based on North American cultivars and one comprised of Kazakh/Siberian and Chinese cultivars. Lines developed in Mexico were spread across these two groupings. Evidence suggests that considerable scope exists to improve bread wheat adaptation at high latitudes globally through intercrossing materials originating from Asia and North America.