葛根高产高效栽培技术

根据多年种植葛根的生产实践,总结其主要生物学特性及高产高效栽培技术。     

Pharmacokinetic study of luteolin,apigenin, chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract in rats

Flos Chrysanthemi (the flower of Chrysanthemum morifolium Ramat.) is widely used in China as a food and traditional Chinese medicine for many diseases. Luteolin and apigenin are two main bioactive components in Flos Chrysanthemi, and chrysoeriol and diosmetin are two methylated metabolites of luteolin in vivo by cathechol-O-methyltransferase (COMT). However, there was lack of pharmacokinetic information of chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract (FCE). The present study aimed to develop an HPLC-UV method for simultaneous determination of rat plasma concentration of luteolin, apigenin, chrysoeriol and diosmetin and utilize it in pharmacokinetic study of the four compounds after orally giving FCE to rats. The method was successfully validated and applied to the pharmacokinetic study when oral administration of FCE to rats with or without co-giving a COMT inhibitor, entacapone. Chrysoeriol and diosmetin were detected in rat plasma after oral administration of FCE and their concentrations were significantly decreased after co-giving entacapone. Furthermore, AUC of luteolin was significantly increased by entacapone, while that of chrysoeriol was decreased by entacapone, which revealed COMT might play an important role in the disposition of luteolin in rats after dosing of FCE. In conclusion, a sensitive, accurate and reproducible HPLC-UV method for simultaneous determination of luteolin, apigenin, chrysoeriol and diosmetin in rat plasma were developed, pharmacokinetics of chrysoeriol and diosmetin combined with luteolin and apigenin were characterized after oral administration of FCE to rats, which gave us more information on pharmacokinetics and potential pharmacological effects of FCE in vivo.     

复方金银花对鸡体内NDV分布的影响

选用1日龄雏鸡,无污染环境饲养21 d后,随机分成感染组、中药治疗组、空白组。中药治疗组饮水给予复方金银花制剂。以单克隆抗体介导的免疫组化（IHC）法检测攻毒鸡体内NDV抗原的分布及定位,以确定复方金银花对新城疫病毒感染鸡的组织嗜性的影响。结果显示,感染组和中药治疗组鸡的16个组织器官中均能检测到NDV抗原,病毒以气管、肺脏、十二指肠、空肠等器官中分布为最高。复方金银花对新城疫的组织损伤有一定的保护作用,可缩短组织修复的时间。     

广州地区粉葛拟锈病病原鉴定

近年在广州地区粉葛上发现一种症状极似豆科锈病的病害,经采样分离致病性测定,显微镜和电镜观察以及田间病害调查结果,认为引致该病的病原菌为葛藤集壶菌（ＳｙｃｈｙｔｒｉｕｍｐｕｅｒａｒｉａｅＭｉｙ）。     

金银花、山银花绿原酸类提取物体外抗NDV作用研究

为研究比较价格差异较大的金银花和山银花的体外抗病毒作用,分别提取金银花和山银花绿原酸类活性成分,利用Vero细胞体外培养系统,通过观察细胞病变效应（CPE）,并用MTT法检测细胞活性来评价和比较金银花和山银花绿原酸类提取物体外抑制新城疫病毒（NDV）对细胞的感染作用。结果表明：在安全浓度范围内,金银花和山银花绿原酸类提取物均具有显著的抗病毒作用,体外对NDV分别具有明显的阻断作用、抑制作用和中和作用,且两者之间的抗病毒效果差异不显著。本试验为价格相对便宜的山银花在一定领域部分代替金银花提供了实验依据。     

天然抑菌剂的提取工艺及抗芒果病原菌作用研究

[目的]研究金银花、槐米、广藿香所组成的复方天然药物中抗芒果病原菌成分的提取工艺和抑菌作用。[方法]对金银花、槐米、广藿香提取工艺中的乙醇浓度、提取次数、提取时间、乙醇用量等关键因素采用正交试验进行优化;最低抑菌浓度采用平板扩散法测定。[结果]金银花、槐米、广藿香三者比例为1∶1∶2配伍使用时,抑菌效力最佳;优选的提取工艺参数为：6倍量70%乙醇,回流提取2次,每次30 min;提取物对胶孢炭疽菌和拟茎点霉最低抑菌浓度为6.25 mg/ml,对球二孢菌最低抑菌浓度为12.50 mg/ml。[结论]提取工艺稳定可行,提取物对芒果典型致病菌有较强的抑制作用。     

产地加工方法对山银花药材品质的影响

〔摘要〕目的比较不同产地加工方法对门!银花药材外观及其有效成分绿原酸含量的影响、方法采用HPLC 法,测定6种产地加工方法的山银花含量〔结果不同产地加工方法的药材中外观及其绿原酸含量相差悬殊,绿原 酸的含量从高到低依次为烘烤法>蒸制干燥法>熏硫干燥法>炒制干燥法>生晒法>晾干法、结论烘烤法加工的药材 外观质量最优.绿原酸含量最高.应)‘一泛推)‘一和应用、     

葛根菠萝保健饮料的加工工艺

为开发葛根深加工产品,用葛根汁和菠萝汁为原料,将其按一定的配比制成了一种天然保健饮料。为了明确葛根菠萝饮料的最佳生产工艺,以葛根汁与菠萝汁体积比、糖添加量、柠檬酸添加量3个因素为考察对象,采用L9（3^3）正交试验设计,研究了葛根菠萝保健饮料的最佳配比方案,并对结果进行方差分析。结果表明：最佳配方为葛根汁与菠萝汁体积比2：3、糖添加量8％、柠檬酸添加量0.12％;方差分析结果表明,葛根汁与菠萝汁配比、糖添加量和柠檬酸添加量对试验结果均有显著影响。在该工艺条件下制得的饮料风味独特,兼具营养保健功效。     

忻城县金银花种质资源调查及绿原酸含量比较

调查了忻城县金银花种质资源情况。结果表明,忻城县共有红腺忍冬、毛花柱忍冬和细毡毛忍冬3种金银花分布。不同金银花样品的绿原酸含量和千花蕾鲜重／干重存在品种间和地区间差异,且随海拔升高而增加。毛花柱忍冬经济性状综合积分最高,其次为红腺忍冬。     

大孔树脂纯化葛花总异黄酮的工艺条件研究

以葛花总异黄酮含量及回收率为考察指标,选用大孔树脂对葛花总异黄酮进行分离纯化。结果表明,X-5树脂对葛花总异黄酮有较好的吸附和解吸效果,最佳工艺条件为:洗脱剂为70%乙醇;洗脱剂用量为3倍树脂体积;流速为2 mL/min;葛花总异黄酮上样液的质量浓度为2 mg/mL,速率为2 mL/min;冲洗杂质用水体积为20 mL。试验表明,该法简单可行,分离效果好,经X-5处理后的葛花异黄酮含量可达54%。