矮牵牛花器官突变体blind-like的表型和解剖结构

 从矮牵牛自交系中获得一个花器官变异突变体, 将其命名为blind-like。该突变体的性状能够稳定遗传, 主要表现为花数增多, 花瓣顶端出现花药状结构, 具有类似正常花药的四分室结构, 并产生少量花粉。该突变体与矮牵牛花器官blind突变体相似, 通过自花授粉与异花授粉产生种子。     

茶屋谈花——茶道插花的风格特点

茶道插花,是东式插花的延续,融入了茶道之神性.在现代花艺发展过程中, 茶道插花表现出的主要风格特点是:线条优美的造型、简洁淡雅的用材、自然清新的情趣、诗情画意的意境.     

甜樱桃PavMYC2基因克隆与表达分析北大核心CSCD

【目的】克隆甜樱桃PavMYC2基因并研究其表达模式,为深入研究其在花芽响应温度逆境中的功能奠定基础。【方法】从甜樱桃基因组数据库中获得PavMYC2基因的序列,对该基因进行生物信息学分析和亚细胞定位;通过qRT-PCR技术探究PavMYC2基因在甜樱桃不同组织及花芽各个时期的表达模式;运用双分子荧光互补实验(BiFC)检测其与PavJAZ蛋白的互作关系;结合前人转录组结果分析PavJAZ的季节表达模式。【结果】PavMYC2编码689个氨基酸,具有bHLH-Zip保守结构域,属于bHLH家族。亚细胞定位结果表明,该基因在细胞核中发挥功能。进化树结果表明,甜樱桃PavMYC2与中国樱桃(Prunus pseudocerasus)和樱花(Prunus yedoensis)亲缘关系最近。实时荧光定量PCR结果分析发现,PavMYC2的表达具有组织特异性,在花芽中表达量最高,依次是叶、茎、花、根中表达量的4.8、4.9、8.8、37.4倍。在花芽中,PavMYC2的表达量在夏秋季高,然后逐渐降低,在冬季维持一定的表达水平,春季时最低。顺式作用元件分析发现,该基因启动子上含有大量光响应元件、多种激素响应元件和低温胁迫等相关元件。互作蛋白结果显示,PavMYC2可以与PavJAZ1/2/3蛋白发生互作,进一步对JAZs基因的表达模式进行分析,发现PavJAZ1/2/3/5与PavMYC2的表达量变化相似。【结论】克隆得到1个PavMYC2基因,夏秋季高温时期在花芽中高表达,随后逐渐降低,在冬季维持一定的表达水平,春季开花时最低。其与PavJAZ1/2/3互作,协同响应温度胁迫。该研究为进一步探究甜樱桃花芽中MYC2在响应温度胁迫和调控开花进程中的作用奠定了基础。     

‘四季蜜’龙眼花芽总蛋白质提取方法及双向电泳体系的优化

利用TCA丙酮法、丙酮法、TCA酚抽提法和改良酚抽法等4种方法,提取‘四季蜜’龙眼花芽的总蛋白质,在蛋白产量、单向SDS-PAGE和2-DE图谱等方面进行比较,并对IPG胶条种类、蛋白质上样量及等电聚焦条件等进行优化。结果表明:采用改良酚抽法,选用24 cm、pH3~10的胶条,按120μg上样,在适宜的等电聚焦条件下,可获得背景清晰、重复性好、分辨率高的2-DE图谱,本研究为‘四季蜜’龙眼花芽蛋白质组学后续研究奠定了基础。     

黄花草木樨花内蜜腺的形态解剖学研究

黄花草木樨的花内蜜腺位于九枚联合雄蕊的花丝基部，并向下延伸到整个花托表层，属于环状花托／雄蕊蜜腺。蜜腺由分泌表皮和产蜜组织组成。分泌表皮上分布着许多呈张开状态的气孔器．蜜汁最终通过气孔泌出植物体外。组织化学试验表明，蜜腺组织细胞内的淀粉粒是蜜汁的主要物质来源之一。     

水稻花器官发育的分子机理

在水稻上,花器官发育是从营养生长转向生殖生长的重要阶段,也是繁衍后代的必然过程,因此是生物学研究的重点与热点。通过对拟南芥、金鱼草等植物的研究,人们了解了植物花器官发育的某些重要特征,克隆了许多相关基因。本文综述了植物花器官发育的ABCDE模型,较详细地总结了参与水稻花器官发育的MADS-box基因及其功能,并介绍了近年来报道的一些水稻花器官突变体及相关基因。     

不同低温胁迫下野扁桃与栽培扁桃花原基解剖结构观察

以新疆野扁桃和4个栽培扁桃为材料,采用石蜡切片法对不同低温胁迫下扁桃的雌、雄蕊原基解剖结构进行观察。结果表明,野扁桃的胚珠和花药原基在厚度、细胞大小、细胞间隙上极显著小于栽培扁桃。扁桃花药和胚珠原基的细胞大小顺序为纸皮>双软>Thompson>Nonpareil>野扁桃。随着温度的降低,扁桃种与品种间胚珠和花药原基的细胞越来越大,细胞间排列越来越疏松,细胞间隙越来越大,细胞受寒害影响加重,抗寒性逐渐降低,相同温度下野扁桃的抗寒性明显强于栽培品种Nonpareil、Thompson、纸皮和双软。     

Variation in concentrations of major bioactive compounds in Hypericum perforatum L. from Lithuania

The growing demand for the medicinal plant, Hypericum perforatum, is not being met by available supply in many countries. The conditions for obtaining quality crude drug should be thoroughly studied to ensure adequate supply. The current study describes the variation of major bioactive compounds in different accessions of H. perforatum (Hypericaceae) in two floral development stages. Two extraction procedures were evaluated for the quantification of the major bioactive compounds in H. perforatum. The Soxhlet extraction technique was demonstrated to be more effective with higher extraction yields. The contents of rutin, hyperoside, quercitrin, quercetin, and 3,8″-biapigenin were determined by high performance liquid chromatography (HPLC). This procedure was used to establish the variation in the contents of flavonoids in the field-growing H. perforatum and to evaluate factors predetermining that variation with some practical implications for utilization. A significant difference in flavonoid contents between two floral development stages was detected. The results of the study showed that rutin prevailed during the budding stage, whereas quercitrin, quercetin, and 3,8″-biapigenin were dominant during the full-flowering stage. The contents of hyperoside were similar in both developmental stages of the plants. The highest amounts of the most bioactive compounds were observed in the full-flowering stage, which could therefore be considered the best stage for the harvesting of the raw material of H. perforatum, known as Hyperici herba in pharmaceutics. The study revealed evident intraspecific variations in the flavonoid contents of H. perforatum. Several chemotypes were recognized by qualitative and quantitative differences in flavonoids. Qualitative analysis of the flavonoid contents confirmed the presence of the chemotype of H. perforatum containing no rutin, though the chemotype with higher levels of both rutin and hyperoside was more frequent. The accessions of H. perforatum also showed remarkable differences in chemical composition depending on the origin of plants. Therefore, employing the correct developmental stage for the harvesting of the selected accessions of H. perforatum is highly recommended for the quality production of the drug Hyperici herba.     

城市道路花卉设计与应用

从设计和应用的角度,研究城市道路花卉景观的特点和作用。文中分花卉设计的应用原则、应用形式和设计实例三方面进行初步探讨,从而希望在城市道路花卉设计的实践中,总结出具有地方特色,适合现代城市新面貌的道路花卉景观,发挥最大经济效益、社会效益和生态效益。     

不同基质对郁金香切花主要性状的影响

[目的]探索栽培基质对郁金香切花主要外观品质的影响。[方法]以菜园土、草炭及珍珠岩等基质为试材,按单一或不同比例进行混合配制成7种栽培基质,研究不同栽培基质对郁金香切花茎高、茎粗及花朵纵径等主要性状的影响。[结果]在菜园土∶草炭=1∶1基质中郁金香花茎最长、茎最粗,分别达53.78和0.95 cm,在菜园土∶珍珠岩∶草炭=1∶1∶1的基质中郁金香花朵纵径最长,为7.91 cm。[结论]在7种栽培基质中,以菜园土∶草碳土=1∶1为栽培基质较好,可以在连云港市推广使用。