Guidance of groups of guppies (Poecilia reticulata) to allow sorting by computer vision

Prior to marketing, ornamental tropical fish are currently sorted, graded and counted manually; a process that is labor intensive, stressful to fish and inaccurate. Manipulation of fish movements at our will is a key element in the development of a device for automatically sorting, grading and counting fish by a computer vision system, and the present study is one step towards the development of such a fish-friendly sorting device. The positive phototactic and rheotactic innate responses of guppies (Poecilia reticulata) were exploited to induce them to swim through transparent pipes or narrow channels, to render them ready for inspection by a computer vision system. Water flow direction and velocity, water level, and illumination were effective in inducing fish to move from one container into the other via a narrow transparent pipe. It was attempted to separate guppies moving in groups into individually spaced fish, by introducing an obstacle into a narrow channel or narrowing the transparent pipe.     

Tuna viscera protein hydrolysate: nutritive and disease resistance properties for Persian sturgeon (Acipenser persicus L.) larvae

The effect of fish protein hydrolysate (FPH) on the growth, feed utilization, digestive enzymes, gut microflora and resistance to bacterial challenge in Persian sturgeon (Acipenser persicus) larvae during a 45‐day feeding trial has been investigated. Artificial diets containing different levels of tuna visceral protein hydrolysate (PH0, PH10, PH25 and PH50) and live foods (Artemia: 5 days, Daphnia: 40 days) were fed to Persian sturgeon larvae. Larvae fed on the PH10 and PH25 had growth, feed utilization, protein content and trypsin activity significantly better than PH0 and PH50 groups (P < 0.05). Condition factors, survival rate and pepsin activity were not significantly different (P > 0.05). Brush border membrane enzymes to cytosolic enzyme ratio revealed that maturation had been occurred in 41 days post hatch (dph) at moderate FPH levels. In 54 dph larvae, this ratio was not affected by the level of FPH in the diet. No significant differences were observed in the total number of gut microflora or in the resistance to Aeromonas hydrophila (P > 0.05), whereas lactic acid bacteria was significantly higher in PH50 (P < 0.05). The results of this study show that application of moderate levels of FPH in Persian sturgeon diets could enhance larvae performance due to the early maturation in intestinal.     

高产池塘混养鱼类关系的研究

应用灰色关联度分析法分析了影响顺德高产池塘鱼产量的主因子,并对顺德高产池塘的多品种混养鱼类关系进行分析,各种不同鱼类的放养对产量的关系为：草鱼放养量对鲢的净产量影响最大（Ｒ＝０．８２４２）其次为鳙放养对草鱼的净产量影响（Ｒ＝０．８０７５）最小为鲮的放养对鳙的净产量关系（Ｒ＝０．２１０２）鳙的放养为优势母因子,鲢的收获为优势子因子。     

大鹏澳网箱养殖海域水质评价及因子分析

根据2001年6月至2002年6月对大鹏澳网箱养殖海域6个航次的水质监测数据,采用有机污染评价指数法对养殖区水质状况进行了评价,同时应用因子分析方法对T(水温)、DO、SS(悬浮物)、CoDMn、BOD5、NO2-N、NO3-N、NH3-N、TIN(总无机氮)、PO4-P等10个水质成分的监测数据进行了分析。结果表明,(1)2001年9月和2002年3月海水出现轻度污染,前者主要是由NO3-N、CoDMn和BoD5所引起,后者是由NH3-N、PO4-P引起的。(2)水质监测数据的总体方差主要来源于T、Do、SS、NO3-N及TIN等5个成分,其次是NO2-N、NH3-N、PO4-P,再次是CODMn和BOD5。(3)因子分析结果表明,各水质成分之间存在一定的相关关系。其中,T、Do、SS、NO3-N及TIN同时载荷于第1主因子上,说明此5个水质成分之间具有较好的相关性;NO2-N、NH3-N、PO4-P同时载荷于第2主因子上,则此3个水质成分之间的相关程性较好;CODMn和BOD5同时载荷于第3主因子上,二者之间具有较好的相关性。     

显微注射技术在制备鱼类嵌合体和转基因海水鱼上的应用

以鱼类胚胎细胞和胚胎干细胞为核供体进行细胞移植构建鱼类嵌合体研究方面,现有的成功报道均采用显微注射方法;在转基因海水鱼类研究中,显微注射也是最为常用的技术,本实验室在花鲈胚胎干细胞嵌合体构建和外源基因向花鲈胚胎的转移研究中取得的结果也充分证实,显微注射技术是开展海水鱼类细胞移植和转基因研究的首选技术。     

鱼类粘液细胞研究进展

鱼类粘液细胞(ｍｕｃｏｕｓｃｅｌｌｓ)是普遍存在于鱼类上皮中的一种腺体细胞,主要分布在鱼的皮肤、鳃及消化道的上皮中,能分泌大量粘液。粘液中含有多种活性物质,如粘多糖、糖蛋白、免疫球蛋白及各种水解性酶类等,对鱼的许多生理功能有重要影响。对粘液细胞进行深入研究,不仅有助于对鱼类生长、发育以及自我保护等方面的一些基本理论问题的理解,而且在鱼类的养殖和病害防治中也具有重要的实践意义。有关鱼类粘液细胞的研究,国外开展得较早,也较广泛,已对多种鱼类的粘液细胞进行了研究。但是,国内有关这方面的研究却很少。本文结合本实验室…     

黑龙江勤得利湾鱼类区系组成特征

初步调查勤得利湾鱼类共计58种，隶属于15科，其中鲤科鱼类为最多，43种，占水系鱼类总数74．13％；鳅科5种，占0．09％；偿科、鲶科各2种，各占0．035％；鲟科、狗鱼科、鳕科、鮨科、塘鳢科、鳢科、杜父鱼科各1种，共占0．017％。肉食性鱼类种类相对较多。通过比较分析，黑龙江勤得利湾鱼类组成与黑龙江中游表现出很大的相似性。     

A computer vision system to analyse the swimming behaviour of farmed fish in commercial aquaculture facilities: A case study using cage-held Atlantic salmon

Knowledge of fish behaviour plays an important role in aquaculture farm management. Video systems are the most common and cost-effective way of observing behaviours in commercial aquaculture operations. However long term observation is not feasible due to a limited ability to analyse footage manually. This paper describes preliminary findings obtained via computer vision software that was developed to automatically analyse fish movement and behaviours in aquaculture sea cages. Results show that the system is capable of detecting fish shapes in video recordings and from these shapes quantifying changes in swimming speed and direction continuously throughout the day. Also variations between days were detected and these may have been associated with the daily shift in the tidal cycle. The system has the potential to act as an alarm to farm operators, informing them about unusual fish behaviours on a continuous, real-time basis. It also has potential to assist in the evaluation of fish welfare.     

First record of infection by Trypanosoma sp. of Colossoma macropomum (Serrasalmidae), a neotropical fish cultivated in the Brazilian Amazon

Fish farming has grown rapidly in Brazil over the last two decades, due in part to the availability of water and species with potential for cultivation, such as Colossoma macropomum. This farmed fish, however, can be affected by parasitic infections with high mortality rates (>35%). The main objective of the present study was to describe the first occurrence of the Trypanosoma species in Colossoma macropomum, which occurred in fish cultured in the Brazilian Amazon region, as well as to morphologically characterize the trypomastigotes and the impact of these hemoparasites on the body condition of the hosts. The trypanosomes found in the blood of Colossoma macropomum were morphologically characterized by the size of the trypomastigote form, indicating the presence of only one morphotype. Of the 39 hosts examined from one fish farming, 41.0% were infected by Trypanosoma sp., with low levels of infection (mean blood density of 3.7/μL). The condition factor of the asymptomatic hosts was not affected by hemoparasitism. There was no correlation between the abundance of Trypanosoma sp. and the condition factor and size of the hosts. Finally, our understanding of host-parasite interactions and the detection of emerging diseases are fundamental for aquaculture.