排序方式: 共有94条查询结果,搜索用时 15 毫秒
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为优化用于口蹄疫灭活疫苗蛋白质含量测定的改良Lowry法,进而确立口蹄疫灭活疫苗蛋白质含量测定的操作程序,探索了有机溶剂破乳剂、酚红以及丙酮沉淀对测定结果影响。结果表明:样品中含酚红和有机溶剂均导致测定值较标准值高;有机溶剂破乳后,水相样经过丙酮沉淀测定值较标准值低;丙酮直接沉淀疫苗后测定蛋白质值与标准值符合度最高,丙酮沉淀回收率随蛋白浓度升高而升高,回收率在90%~100%之间。试验首次确立了改良Lowry法检测口蹄疫灭活疫苗中蛋白质含量的操作程序为丙酮直接沉淀疫苗后测定蛋白质浓度。并成功应用于口蹄疫灭活疫苗蛋白质含量的测定。 相似文献
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用口蹄疫A型病毒AF/72株的第3代乳鼠组织毒,通过乳鼠适应至6代,获得该毒株的乳鼠组织毒AF/72/MF6,经测定其LD50为10^-8.0·mL^-1;经RT-PCR获得其VP1基因序列,并与GenBank中的其它6株口蹄疫A型病毒株比对,同源性大于85%;经无菌检验和外源病毒检验,纯净性达到兽用生物制品标准要求;经间接夹心ELISA测定,OD值均大于0.2,且经乳鼠中和试验证实该毒仅能被口蹄疫A型标准血清中和,具有型特异性;经紫外分光光度法测定其146S含量,均值为189 ng·mL^-1,远大于22 ng·mL^-1的国际标准。综合纯净性检验、特异性检验和146S含量测定结果,可确定AF/72/MF6为口蹄疫A型病毒AF/72株乳鼠组织毒的标准毒。 相似文献
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口蹄疫疫苗的有效抗原成分为完整的口蹄疫病毒粒子(146S),疫苗的效力与疫苗中含有的146S具有极大的相关性。因此对疫苗的生产过程及产品进行146S的定量检测是非常重要的环节。目前对146S定量的方法主要是超速离心的方法,除此之外,ELISA检测也越来越受到关注,同时很多研究人员也在积极探索其他更为简便快捷的检测方法。作者主要对各种146S的定量方法作一综述。 相似文献
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Yosuke SASAKI Satoshi SEKIGUCHI Ryoko UEMURA Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):333-335
This study compared the reproductive and growth performances of pigs before and after depopulation andrestocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and afterdepopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation andrestocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive andrespiratory syndrome and Aujeszky’s disease, remained undetected on the farms. Compared with beforedepopulation, reproductive and growth performances improved after depopulation; the number of total pigs bornwas higher, the postweaning mortality rate was lower, and the age at slaughter was lower(P<0.05). In summary, depopulation and restocking improved the reproductive and growthperformances of pigs. 相似文献
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Harvey N Reeves A Schoenbaum MA Zagmutt-Vergara FJ Dubé C Hill AE Corso BA McNab WB Cartwright CI Salman MD 《Preventive veterinary medicine》2007,82(3-4):176-197
The North American Animal Disease Spread Model is a stochastic, spatial, state-transition simulation model for the spread of highly contagious diseases of animals. It was developed with broad international support to assist policy development and decision making involving disease incursions. User-established parameters define model behavior in terms of disease progression; disease spread by animal-to-animal contact, contact with contaminated personnel or equipment, and airborne dissemination; and the implementation of control measures such as destruction and vaccination. Resources available to implement disease control strategies, as well as the direct costs associated with these strategies, are taken into consideration. The model records a wide variety of measures of the extent of simulated outbreaks and other characteristics. The graphical interface and output visualization features also make it a useful tool for training and preparedness exercises. This model is now being used to evaluate outbreak scenarios and potential control strategies for several economically important exotic animal diseases in the United States, Canada, and elsewhere. NAADSM is freely available via the Internet at http://www.naadsm.org. 相似文献
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口蹄疫诊断技术的研究进展 总被引:2,自引:0,他引:2
综述了口蹄疫的诊断方法,包括临床诊断、病毒分离、血清学、分子生物学及一些新型的诊断技术,尤其对ELISA、PCR和基因芯片等快速、灵敏的诊断技术做以阐述,对其优缺点进行比较。 相似文献
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为了考察市售高效灭活疫苗与政府采购疫苗免疫效果之间差异分别选取了两家疫苗公司生产的政府采购疫苗和高效灭活疫苗,分别在4个猪场展开免疫效果评估。4个猪场按照相同的免疫程序进行免疫,分别采集免疫前、加强免疫后30 d、加强免疫后60 d的血清,用O型口蹄疫抗体液相阻断ELISA试剂盒进行抗体检测。结果表明,加强免疫后30 d,抗体平均效价依次为1∶196.9、1∶251.9、1∶256.0、1∶243.8。高效灭活苗与政府采购疫苗在同样的免疫程序和免疫剂量下,同一时间点其抗体水平效价差异不显著;但免疫副反应方面,高效灭活疫苗比政府采购疫苗低,且差异极显著。 相似文献
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