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金城山草本层淫羊藿植物群落的稳定性分析 总被引:1,自引:0,他引:1
[目的]分析嘉陵江流域金城山草本层淫羊藿植物群落的稳定性.[方法]研究对象是金城山森林群落草本层植物淫羊藿,以及作对照的鸢尾、复叶耳蕨、苔草、卷柏、细柄草.采用随机取样法调查了20个2 m×2 m样方.对每个样方进行草本层主要植物种群的频度、盖度、密度、高度和生物量的测定.最后计算金城山草本层几种主要植物的生态位宽度和生态位重叠.[结果]测定金城山草本层几种主要植物的生态位宽度和生态位重叠结果表明:金城山草本层植物植物群落已经具有了相当的稳定性,但这种稳定性是有限的,还处于动态的变化之中.[结论]对建立农林复合体系,开发淫羊藿中药资源和加强淫羊藿野生资源保护有一定的指导意义. 相似文献
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旨在优化淫羊藿多糖醇沉条件并研究不同浓度乙醇醇沉的淫羊藿多糖对巨噬细胞释放NO的影响。采用乙醇沉淀法从淫羊藿多糖提取物中获得淫羊藿多糖,在单因素试验基础上,选择浓缩比、乙醇浓度、乙醇体积倍数、醇沉时间为自变量,采用L9(34)的正交试验设计,优化淫羊藿多糖最佳醇沉条件。用浓度为400、500、600、700、800mL/L乙醇醇沉的淫羊藿多糖,采用体外培养法,测定淫羊藿多糖对巨噬细胞释放NO的影响。结果表明,淫羊藿多糖最佳醇沉条件为浓缩比为1∶2,乙醇浓度为800mL/L,乙醇体积倍数为4倍,醇沉时间为12h;每个乙醇浓度醇沉的淫羊藿多糖均具有良好的活性,500mL/L乙醇醇沉的多糖活性最好。结果显示,淫羊藿多糖对巨噬细胞具有良好的刺激作用。 相似文献
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两种药用淫羊藿克隆构型与分株种群特征比较 总被引:2,自引:2,他引:0
为探讨淫羊藿与箭叶淫羊藿生境资源利用及生态适应对策的异同,出较了异质性生境中两种淫羊藿克隆生长指标、克隆构型指数(V)和分株种群密度.结果表明,淫羊藿与箭叶淫羊藿的间隔子长度、根状茎长度、分株高度差异显著,分枝强度和分枝角度差异不显著,克隆构型指教V和分株种群密度接近.两种淫羊藿均为"游击型"克隆植物,其地下根茎寿命在2年以上,具有很强的克隆整合性.应减少对2种淫羊藿生境的干扰和保护其地下根茎的克隆生长,才能实现野生淫羊藿资源的可持续性利用. 相似文献
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研究肉苁蓉及淫羊藿对小鼠早期胚胎体外发育的影响.试验选取6~8周龄昆明小鼠早期胚胎,以不添加中药培养液组为空白对照,分以下试验组:Ⅰ组:早期胚胎在纯培养液M16+5%FBS( M1)与M16+5%FBS+EDTA+牛磺酸(M2)中的体外发育情况;Ⅱ组:早期胚胎添加至中药体积浓度为0.1%的M1与M2培养液中.Ⅲ组:早期... 相似文献
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不同采收期、不同海拔朝鲜淫羊藿茎中总黄酮含量测定 总被引:1,自引:0,他引:1
目的测定不同采收期、不同海拔朝鲜淫羊藿茎中总黄酮含量。方法应用紫外分光光度法,以淫羊藿苷为标准品,在270nm下测定吸光度,计算朝鲜淫羊藿茎中的总黄酮的含量。结果在0.06mg~0.42mg范围内,淫羊藿苷质量(mg)与吸光度A值线性关系良好,平均回收率为101.60%,RSD=2.47%,r=0.9991。不同采收期朝鲜淫羊藿茎:6月16日(3.325%),7月13日(3.738%),8月1日(2.958%),8月17日(2.684%),9月2日(5.526%),9月16日(2.090%),10月2日(3.922%);不同海拔的朝鲜淫羊藿,海拔629米(3.509%),海拔805米(2.319%),海拔925米(2.777%)。结论随着采收期的不同茎中总黄酮的含量有所变化。本方法简便可靠、快速、重现性好,适用于朝鲜淫羊藿中总黄酮的含量测定,为进一步研究朝鲜淫羊藿质量评价提供了参考数据。 相似文献
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通过正交试验,对影响淫羊藿多糖提取效率的乙醇体积分数、提取时间、提取温度、溶剂与淫 羊藿量比等因素进行了研究。结果表明,优化工艺为以20%乙醇为溶剂,淫羊藿与溶剂按1:100g/mL 投料,在60℃下提取100min,淫羊藿多糖收率为17.6%。 相似文献
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MA Dong-xue GAO Wei-juan LIU Zhen-yi ZHANG Hong-jun WANG Jia-ying DONG Xian-hui 《园艺学报》2021,36(12):2198-2204
AIM To explore the effect of compound of Epimedium , Astragalus and Radix Puerariae on the expression of a disintegrin and metalloproteinase 10 (ADAM10) in Aβ-induced hippocampal neuron HT22 cells with or without hepcidin (HAMP) expression knock-down for analyzing the pathogenesis of Alzheimer disease (AD) at cell level. METHODS Hippocampal neuron HT22 cells were cultured in vitro and randomly divided into 7 groups: control group, Aβ group (Aβ25-35-induced HT22 cells), RNAi group (HAMP gene was silenced in HT22 cells), Aβ+RNAi group (HAMP gene expression in Aβ25-35-induced HT22 cells was silenced), Aβ+TCM group (Aβ25-35-induced HT22 cells were treated with Epimedium , Astragalus root and Radix Puerariae effective components), RNAi+TCM group (HT22 cells with HAMP gene silence were treated with Epimedium , Astragalus root and Radix Puerariae effective components) and Aβ+RNAi+TCM group (Aβ25-35-induced HT22 cells with HAMP gene silence were treated with Epimedium , Astragalus root and Radix Puerariae effective components). The silence efficiency of HAMP siRNA was detected by qPCR and Western blot. The ADAM10 expression in each group was determined by immunofluorescence, qPCR and Western blot. RESULTS The HAMP siRNA-3 sequence had the highest interference efficiency. Compared with control group, the expression levels of ADAM10 in Aβ group, RNAi group and Aβ+RNAi group were decreased (P <0.05). Compared with Aβ group,the expression levels of ADAM10 in Aβ+RNAi group was also decreased (P <0.05), and the expression levels of ADAM10 in Aβ+TCM group was increased (P <0.05). Compared with RNAi group, the expression levels of ADAM10 in Aβ+RNAi group was decreased (P <0.05), while the expression levels of ADAM10 in RNAi+TCM group was increased (P <0.05). Compared with Aβ+RNAi group, the expression levels of ADAM10 in Aβ+RNAi+TCM group was increased (P <0.05). CONCLUSION The effective components of Epimedium , Astragalus and Radix Puerariae compound promotes the expression of ADAM10 in Aβ25-35-induced HT22 cells, which mechanism may be related to the expression of HAMP. 相似文献
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