应用BRD药剂防治香蕉簇矮病的研究

应用混配的BRD药剂防治香蕉簇矮病,取得了令人满意的效果。移栽前,用1:2000的BRD901药液浸渍带毒的吸芽苗,平均脱毒效果可达到80.2％;用1:300的BRD902药液淋施初发病株,平均防治效果也可达到83.3％,而且治愈的病株不再复发,在生产上可以进一步推广应用。     

中国矮秆棉花的研究现状

棉花矮化品种具有抗倒伏能力强、适宜密植及机械化管理的特点,对于减小劳动强度、提高肥料利用率和提高产量具有重要意义,矮化突变体在矮化育种中具有重要的利用价值,同时也是研究植物茎的生长及激素调控的重要基因资源。近年来国内不断有棉花矮秆突变体发现的报道,相关专家对其进行了遗传方式、矮化机理和育种等方面的研究。     

Relationships between the number of dwarf shoots of<Emphasis Type="Italic"> Larix decidua</Emphasis> Mill. damaged by different insect species in the Ojców National Park in southern Poland

A study was conducted on the interrelationships between the number of dwarf shoots of Larix decidua Mill. damaged by insects: Adelges laricis Vall. Coleophora laricella Hbn., Spilonota laricana Hein. and Dasineura kellneri (Hensch.). The study made use of dwarf shoots collected at three sites in the Ojców National Park, southern Poland during 2002–2003. A total of 240 branches with 19,239 dwarf shoots, collected from 60 larches were designated to the study. The data obtained were subjected to statistical analysis using the Spearman rank correlation coefficient and the Students t-test at the 0.05 level. No unequivocal correlation was found between the relationships.     

Tree regeneration in partially cut conifer–hardwood mixed forests in northern Japan: roles of establishment substrate and dwarf bamboo

We assessed the relationship of the regeneration (seedling and sapling) densities of seven representative tall-tree species to the past partial cutting and current stand structure. We also determined if differences in tree regeneration were associated to establishment substrates (coarse woody debris (CWD) and root throws) and understory inhibitor vegetation (the dwarf bamboo species: Sasa senanensis). The study was conducted in 17 conifer–hardwood mixed stands in a heavy snowfall region in Hokkaido, northern Japan. The results suggest that stand structure, rather than logging intensity, is the primary factor influencing regeneration densities. Total conifer basal area was positively correlated with the regeneration density of individual tree species, including two conifers and three hardwoods. These patterns differ from those observed in old-growth stands in the region. A negative correlation between total conifer basal area and dwarf bamboo coverage suggests that the presence of dense conifer canopies causes an increase in regeneration density of tall-tree species by preventing domination of dwarf bamboo. Picea glehnii, a species that depends for its establishment strongly on CWD, has lower seedling and sapling density in stands with higher logging intensity. This seems to be a result of the decrease in the volume of CWD with increasing logging intensity in these stands. We suggest that both reducing logging intensity and retaining overstory conifers should be considered to develop a sustainable silvicultural system in this region. Providing sufficient CWD and root throws may also be important to ensure natural regeneration of tree species that require these as an establishment substrate.     

山东苹果M26矮化砧木栽培适应性调查与评价

对山东省17个县(市)苹果M26矮化砧木的生产表现进行了系统调查,并对其栽培适应性做出了评价。结果表明:以M26为中间砧嫁接的富士,幼树生长势强,大树树体稳定性好,在山东省大部分地区表现出良好的抗寒性、抗旱性、抗风折性。     

宁夏矮败小麦的利用研究

矮败小麦是具有矮秆基因标记的太谷核不育小麦,利用矮败小麦进行轮回选择,是一种高效的小麦群体改良方法。通过对矮败小麦的引进、研究与利用,结合宁夏小麦育种的特点,先后创建了高产、优质、早熟、抗旱等4个轮回群体,从中选育了一批优秀的后代材料和亲本创新材料。     

橡胶树转HbCBF1基因矮化突变体T-DNA插入位点研究

利用经PCR、GUS染色和Southern杂交均证明已成功转入Hb CBF1的、田间表型为矮化的橡胶树植株C1为材料,通过TAIL-PCR的方法获得T-DNA右侧侧翼序列1.6 kb,根据侧翼序列与载体的序列设计引物分析验证该序列真实可靠,并设计引物判断基因的T-DNA插入以杂合位点存在,与此同时,将该侧翼序列与已有的橡胶树基因组测序结果进行比对分析,发现该插入位点处并不存在基因,根据CBF1与Ca MV35s启动子的研究推测,C1植株的矮化表型是由于Ca MV35s启动子驱动抗逆基因导致,而非插入基因造成。     

甜樱桃砧木吉塞拉(Gisela)叶片再生体系研究

 以甜樱桃矮化砧木‘Gisela 5’和‘Gisela 6’的组培苗为试材，研究建立叶片的离体再生体系。结果表明，Gisela 6的再生能力高于Gisela 5；叶片正面接触培养基比背面接触培养基更有利于分化再生。用新梢顶端1~3节新展开的叶片制备外植体，分别接种在WPM+BA 7 mg/L+IBA 0.3 mg/L和WPM+BA 5 mg/L+IBA 0.5 mg/L的培养基上，黑暗处理15 d，Gisela 5的最高分化频率为75.1%，Gisela 6为100%。再生芽苗在三角瓶中生根容易，移人大田后生长正常。     

小麦黄化型病毒病的研究

利用蚜虫传播、免疫电泳和超薄切片的电镜观察等方法对小麦田间新出现的四种黄化型病毒病进行了系统研究,确定是由大麦黄矮病毒所致,而不是小麦黄叶病。禾谷缢管蚜需要6小时可完成传毒,平均发病率为85%。大麦黄矮病毒粒子只存在和复制于植物韧皮部组织的筛管和伴胞内,病毒粒子大量聚集在细胞质内并在细胞质内形成“扭结”线状内含体。     

小麦矮秆基因Rht#-10对赤霉酸反应的研究

利用赤霉酸（GA#-3）对矮变一号的幼苗及胚乳进行了处理。结果表明,矮变一号不仅植株的地上部分,而且其胚乳组织对赤霉酸都是不敏感的;从两份不同来源的矮变一号对赤霉酸反应的差异及2x中7902单体/矮变一号BC#-1的表现,推测矮变一号赤霉酸不敏感性除受Rht#-10主效基因控制外,还受其他微效修饰基因的影响。