全文获取类型
收费全文 | 1956篇 |
免费 | 50篇 |
国内免费 | 123篇 |
专业分类
林业 | 67篇 |
农学 | 108篇 |
基础科学 | 37篇 |
121篇 | |
综合类 | 899篇 |
农作物 | 59篇 |
水产渔业 | 20篇 |
畜牧兽医 | 755篇 |
园艺 | 58篇 |
植物保护 | 5篇 |
出版年
2024年 | 1篇 |
2023年 | 9篇 |
2022年 | 22篇 |
2021年 | 55篇 |
2020年 | 66篇 |
2019年 | 45篇 |
2018年 | 29篇 |
2017年 | 18篇 |
2016年 | 45篇 |
2015年 | 64篇 |
2014年 | 132篇 |
2013年 | 99篇 |
2012年 | 103篇 |
2011年 | 142篇 |
2010年 | 129篇 |
2009年 | 167篇 |
2008年 | 144篇 |
2007年 | 200篇 |
2006年 | 128篇 |
2005年 | 102篇 |
2004年 | 60篇 |
2003年 | 41篇 |
2002年 | 40篇 |
2001年 | 33篇 |
2000年 | 46篇 |
1999年 | 34篇 |
1998年 | 34篇 |
1997年 | 22篇 |
1996年 | 17篇 |
1995年 | 21篇 |
1994年 | 19篇 |
1993年 | 12篇 |
1992年 | 11篇 |
1991年 | 6篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 6篇 |
1987年 | 4篇 |
1975年 | 1篇 |
排序方式: 共有2129条查询结果,搜索用时 15 毫秒
11.
12.
Chie SUZUKI Yosuke SAKAGUCHI Hiroyoshi HOSHI Koji YOSHIOKA 《The Journal of reproduction and development》2016,62(1):79-86
The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced
in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium
(PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates
of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured
with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of
LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with
other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and
Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured
with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes
involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3,
CPT1, CPT2 and KAT) in Day-7 blastocysts were
significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching
ability and quality of porcine blastocysts produced in vitro, as determined by ATP content,
blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of
LR-BSA arise from lipids bound to albumin. 相似文献
13.
自2018年8月以来暴发的非洲猪瘟疫情给我国猪育种和生猪生产带来了极大的困扰,育种体系遭受重创。文章综述了非洲猪瘟对我国育种体系的影响、育种工作面临的困境与挑战以及非洲猪瘟形势下猪育种的对策。 相似文献
14.
桑叶养猪实用技术分为桑树种植技术和桑叶干粉发酵养猪技术两个部分。利用荒地种植桑树,降低生猪养殖饲养成本,桑叶养猪种养结合,成为生态农业典范。桑叶干粉发酵技术,提高了桑叶干粉的可消化率和饲料功能。桑叶干粉发酵料占配方6%~10%,做成桑叶猪饲料,替代3%~5%鱼粉、豆粕等蛋白质饲料,节约了饲料成本。桑叶喂养猪(简称桑叶猪,下同)的猪肉中含有肌苷酸等风味物质,显著提高猪肉胴体品质和猪肉风味。设计猪尿处理,采用红泥膜沼气池降解工程工艺应用后,降解水兑2倍清水对桑树喷灌工艺,减少人工处理劳力和成本费用。设计10%抗非洲猪瘟中药饲料添加剂+桑叶饲料,预期防治非洲猪瘟总效果85%以上。桑叶养猪实用技术可行,供同行参考。 相似文献
15.
在世界上主要的猪肉生产国中,猪人工授精(AI)技术的应用率在90%以上。尽管猪冷冻精液技术在不断进步,但由于其效率不高,在猪场实际生产中并不多见使用。在猪人工授精技术中,液态精液是目前应用最广泛的精液,与传统的牛冷冻精液不同,其独特的精子微胶囊保护膜技术,可以对猪精子进行更加完善的液态保存,因此液态精液是目前猪人工授精使用的主要方式。文章综述了猪精液保存技术在人工授精中的应用,并展望了今后的发展方向,为人工授精技术助力恢复生猪生产提供参考。 相似文献
16.
17.
根据高职院校的人才培养定位,针对《园林植物学》的课程特点,结合课程教学要求,为切实提高学习效果,提出了提高学生学习兴趣的几点建议,以期为其他理论性较强的课程教学提供参考。 相似文献
18.
《Veterinary parasitology》2015,207(1-2):1-6
The success of a Toxoplasma gondii surveillance program in European pig production systems depends partly on the quality of the test to detect infection in the population. The test accuracy of a recently developed serological bead-based assay (BBA) was investigated earlier using sera from experimentally infected animals. In this study, the accuracy of the BBA was determined by the use of sera from animals from two field subpopulations. As no T. gondii infection information of these animals was available, test accuracy was determined through a Bayesian approach allowing for conditional dependency between BBA and an ELISA test. The priors for prevalence were based on available information from literature, whereas for specificity vague non-informative priors were used. Priors for sensitivity were based either on available information or specified as non-informative. Posterior estimates for BBA sensitivity and specificity were (mode) 0.855 (Bayesian 95% credibility interval (bCI) 0.702–0.960) and 0.913 (bCI 0.893–0.931), respectively. Comparing the results of BBA and ELISA, sensitivity was higher for the BBA while specificity was higher for ELISA. Alternative priors for the sensitivity affected posterior estimates for sensitivity of both BBA and ELISA, but not for specificity. Because the difference in prevalence between the two subpopulations is small, and the number of infected animals is small as well, the precision of the posterior estimates for sensitivity may be less accurate in comparison to the estimates for specificity. The estimated value for specificity of BBA is at least optimally defined for testing pigs from conventional and organic Dutch farms. 相似文献
19.
20.