洛阳地区园林常绿阔叶树种资源及其应用

为了对洛阳市的生态环境建设提供参考,对洛阳地区常绿阔叶树种进行了实地调查.结果表明,洛阳地区园林中应用的常绿阔叶树种共有58种,其中,乔木9种,灌木39种、藤本4种及竹类6种;野生常绿阔叶树种10余种.并针对应用中存在的问题,提出相应的发展对策.     

Overload of ferric ammonium citrate triggers MAPK pathways by producing high level of reactive oxygen species and induces apoptosis of human hFOB1.19 osteoblast cells

AIM：To investigate the role of reative oxygen species (ROS) generated by iron overload in activating the mitogen-activated protein kinase (MAPK) pathways and apoptosis. METHODS：Cultured human osteoblast cell line hFOB1.19 was treated with ferric ammonium citrate (FAC) at concentrations of 0~500 μmoL/L. The proliferation of hFOB1.19 cells was analyzed by MTT assay. Apoptosis was detected by flow cytometry with Annexin V/PI staining. The expression levels of p-ERK, p-JNK and p-p38 were determined by Western blotting 24 h after treatment with FAC. RESULTS：After treated with FAC, the cell proliferation was inhibited. The early apoptosis and total cell death were significantly increased. The levels of ROS were increased to (35.73±2.52)%, (62.89±4.24)% and (76.06±3.55)% with the increasing doses of FAC treatmen,respectively. The expression levels of p-ERK, p-JNK and p-p38 were also remarkably elevated in FAC groups. CONCLUSION：Iron overload increases intracellular ROS level, thus triggering the MAPK pathways and inducing apoptosis of human hFOB1.19 osteoblast cells.     

云南省古林箐自然保护区物种多样性及保护

古林箐自然保护区位于北回归线以南,哀牢山南端以东的滇东南高原向东南半岛延伸的结合部,南亚热带与热带的过渡地带。在这特殊的自然地理条件下,成为我国三大生物多样性中心之一的滇东南—桂西北的核心部位,区内有发育完整,保存完好的森林生态系统和丰富的生物资源。但在管理中存在许多方面问题,据此提出保护措施。     

甘肃省兴隆山区域丛枝菌根真菌多样性初探

本研究采用Illumina HiSeq分子测序技术,探究了兴隆山东山丛枝菌根(Arbuscular mycorrhizal,AM)真菌多样性及其在不同海拔高度的分布特征。结果表明:1)自兴隆山东山土壤分离获得AM真菌共52种,隶属于4目8科13属,其中球囊霉属(Glomus)和原囊霉属(Archaeospora)为主要物种,分别为20种和9种,共占总物种的55.77%;2)各海拔梯度AM真菌种属分布结果表明,优势属球囊霉属(Glomus)和类球囊霉属(Paraglomus)随海拔变化呈现规律性分布,其余各属均匀分布于不同海拔梯度;球状巨孢囊霉(Gigaspora margarita)、蜜色无梗囊霉(Acaulospora mellea)、白色球囊霉(Glomus albidum)、球囊霉属的Glomus sp.1和Glomus sp.4,还有弯丝硬囊霉(Sclerocystis sinuosa)仅在部分海拔梯度出现,为不同海拔特有种;3)AM真菌丰富度分析表明,从低海拔到高海拔,ACE和Chao1指数总体上表现出“单峰”变化曲线,且部分海拔间差异性显著(P<0.05)。Spearman相关性分析发现,该地区AM真菌种属分布受海拔因子影响较小,两者间不具有显著相关性。综上,兴隆山东山AM真菌资源丰富,物种繁多,具有较大的应用潜力。     

PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响

为探明东北地区常见野生葱属植物耐旱能力,筛选野生葱属植物耐旱种质资源,本研究以野韭（Allium ramosum L.）、山韭（Allium senescens L.）和蒙古韭（Allium mongolicum Regel）3种葱属植物为材料,采用不同浓度的PEG-6000模拟干旱胁迫,分析其种子萌发期渗透调节物质及酶活性的影响,并结合主成分分析和隶属函数分析比较抗旱性。结果表明：干旱胁迫下,3种葱属植物种子萌发期相对电导率显著升高（P<0.05）,丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量及超氧化物歧化酶、过氧化氢酶、过氧化物酶、多酚氧化酶活性均呈现先上升后下降的趋势。3种植物相比,山韭相对电导率最低,渗透调节物质积累量最高,酶活性变化明显。主成分分析表明,4种酶活性之间显示出强相关性。隶属函数分析得出3种植物种子的抗旱性由高到低依次为：山韭 > 野韭 > 蒙古韭。     

黄河兰州段湿地典型植物群落物种多样性研究

以黄河兰州段分布最广泛的芦苇-假苇拂子茅群落和酸模叶蓼-灰绿藜群落为研究对象,研究2个群落的植物物种组成和多样性特征。结果表明:芦苇群落有植物22种,隶属于13科20属,酸模叶蓼群落有植物24种,隶属于11科22属,2个群落的共种有10种,相似性系数为43.48%,菊科、禾本科、藜科、蓼科是该植物区系的优势科。物种多样性的分析说明2个群落的Pielou指数(J)波动比较小,Simpson指数(D)和Shannon-Wiener指数(H)均波动都比较大,并且H指数与J指数存在明显的正比例关系,而与D指数呈显著的反比例关系。方差分析表现出两群落S,H,D和J指数的差异并不明显,说明组成两群落的物种有一定的相似性和连续性,也存在着差异。     

Livestock grazing has minimal effect on the species richness and diversity of semi-arid shrubland in the Western Little Karoo,South Africa

The Succulent Karoo, one of two arid biodiversity hotspots in the world, is known for its high plant species richness, but little is known about the influence of topography and how it mediates the potentially deleterious effects of grazing. Changes in vegetation species composition, cover and species diversity were examined along piosphere gradients on northerly slopes, bottomlands and low-lying plains on 45 farms. Landscapes differed in the plant speciesand life-form composition, species richness and cover, with the more species-rich, heterogeneous grassy northerly slopes distinct from the plains and bottomlands, which were dominated by Pteronia pallens, Psilocaulon junceum and Drosanthenum spp. with more annual and ephemeral species. Overall, species richness declined linearly with decreasing rangeland condition. A weak grazing effect was detected only on the north-facing slopes, where shrubs and grass decreased with intense grazing. Years of overgrazing have resulted in the widespread dominance of P. pallens, especially on the plains and bottomlands, rendering them insensitive to grazing. The current condition of the northern slopes should be maintained and managed such that palatable species can spread to more degraded areas of the landscape.     

Michigan Mute Swan Management: A Case Study to Understand Contentious Natural Resource Management Issues

U.S. state fish and wildlife agencies have responded to perceived ecological and social threats posed by mute swans by implementing population control strategies. In Michigan, some stakeholder groups have vocally opposed particular control activities. To better understand and characterize key aspects of the underlying media dialogue about mute swan management in Michigan, one factor in the development of public perceptions about wildlife issues, we conducted a qualitative content analysis of Michigan-specific online news articles. Results revealed tensions between stakeholder groups about management priorities were rooted in differing ideas about healthy social and ecological systems, appropriate wildlife behavior, and the acceptability of lethal control (i.e., shooting living birds). Characterizing which stakeholder groups aligned with particular arguments might allow managers to tailor and direct messaging to specific audiences.