奶牛皱胃左方变位不同方法治疗前后瘤胃内纤毛虫数量及pH的变化

采用保守疗法、左侧腹壁切口不切开瘤胃和左侧腹壁切口切开瘤胃治疗皱胃左方变位,治疗前后不同时期对患牛进行瘤胃内纤毛虫数量及内容物pH测定.结果表明,手术治疗采用左侧腹壁切口并切开瘤胃,冲洗、取出网胃内金属异物,手术后1d瘤胃内纤毛虫数量及内容物pH较手术前1d显著下降(P＜0.05),恢复缓慢；采用左侧腹壁切口不切开瘤胃,1d后瘤胃内纤毛虫及内容物pH较手术前1d显著上升(P＜0.05),恢复较快；保守疗法瘤胃内纤毛虫数量及内容物pH变化不显著(P＞0.05)；治疗过程中纤毛虫数量受pH影响较大.     

牛乳溶菌酶概述及应用

溶菌酶又称胞壁质酶,广泛存在于自然界的动物、植物及微生物中。具有抗菌消炎、抗病毒、增强免疫力以及促进组织修复等生物学特性。并且应用于食品、饲料、医学及生物工程之中。针对目前临床中,对于奶牛乳房炎广泛大量使用抗生素,并且存在耐药性、药物残留等现象。重组溶菌酶质粒可以作为基因工程药物替代抗生素对乳房炎的防治,并且安全、无残留、无副作用。同时也还有待于进一步的研发和推广。     

含蛋氨酸二肽影响奶牛乳腺上皮细胞乳蛋白合成相关基因表达

本试验旨在研究含蛋氨酸(Met)二肽对奶牛乳腺上皮细胞(BMECs)内乳蛋白合成相关基因表达的影响。试验分3部分,均采用单因子完全随机试验设计,Met的添加浓度及培养时间分别为60μg/m L(0.402 mmol/L)、48 h。第1部分,培养液添加8种含Met二肽[蛋氨酸-蛋氨酸(P-Met-Met)、蛋氨酸-赖氨酸(P-Met-Lys)、蛋氨酸-色氨酸(P-Met-Trp)、蛋氨酸-苯丙氨酸(P-Met-Phe)、蛋氨酸-苏氨酸(P-Met-Thr)、蛋氨酸-异亮氨酸(P-Met-Ile)、蛋氨酸-亮氨酸(P-Met-Leu)、蛋氨酸-缬氨酸(P-Met-Val)],以不添加二肽为对照,测定BMECs乳蛋白合成相关基因(αs1-酪蛋白、β-酪蛋白、κ-酪蛋白、β-乳球蛋白、Ⅱ型小肽转运载体和氨肽酶氮)的表达量;第2部分,培养液添加8种与上述二肽对应的游离氨基酸(F-Met-Met、F-Met-Lys、F-MetTrp、F-M et-Phe、F-M et-Thr、F-M et-Ile、F-M et-Leu、F-M et-Val),以不添加游离氨基酸为对照,测定BM ECs乳蛋白合成相关基因的表达量;第3部分,用二肽等物质的量替代相应游离氨基酸,测定BMECs乳蛋白合成相关基因的表达量以及细胞内外氨肽酶含量。结果表明:P-Met-Met和P-M et-Lys组较对照组和其他二肽组上调了αs1-酪蛋白和β-酪蛋白基因的表达量,且P-M et-M et组优于P-Met-Lys组。F-Met-Met和F-Met-Lys组较对照组和其他游离氨基酸组显著提高了αs1-酪蛋白基因的表达量(P0.05)。除P-Met-Val和P-Met-Leu组外,其他二肽替代游离氨基酸后均不同程度地提高了乳蛋白和Ⅱ型小肽转运载体基因的表达量,其中P-Met-Met表现出较好的促进效果。总之,含Met二肽等量替代对应的游离氨基酸能够促进乳蛋白基因的表达,其中尤以P-Met-Met的效果最好。     

A comparison of the incidence of tuberculosis in forty-six town supply herds in the manawatu district in 1950–51 and 1951–52

Extract

The forty-six herds concerned were tuberculintested in 1950–51 under the provisions of the appropriate section of the Stock Amendment Act, 1945. The same herds were retested in the season 1951–52 at an interval of approximately one year from the date of the first test.     

乳制品进口对我国乳业发展的影响及有关政策建议.

乳制品进口对满足国内需求、促进我国乳业整体水平的提高和生产结构调整起到一定作用。受乳品质量安全事件频发、生鲜乳标准降低、国内需求不断增加和国际贸易环境的影响,近年来乳制品进口量增加,对我国奶牛养殖业形成压力,使众多乳制品企业出现生存危机,侵害了乳业广大生产者和消费者的利益。本文分析了乳制品进口对我国乳业影响,并提出了有关支持和保护我国乳业发展的政策建议。     

公英翘芦散治疗亚临床型奶牛乳房炎的效果研究

本研究旨在初步考察公英翘芦散对亚临床奶牛乳房炎的临床治疗效果。试验选取78头发病牛，其中38头牛为散发，用公英翘芦散治疗；40头牛集中对比治疗，随机分成2组，每组20头，分别用公英翘芦散和公英散进行治疗。用加州乳房炎检验（California mastitis trial，CMT）法检测公英翘芦散治疗后乳区患病状态并判定其治疗效果；用体细胞计数（somatic cell count，SCC）法检测公英翘芦散和公英散治疗后牛奶体细胞数并判定其治疗效果。结果显示，公英翘芦散给药7 d后，患病乳区由75个减少到28个，治疗效果非常显著，治愈率达到62.67%，有效率达到90.67%。与治疗前相比，公英翘芦散和公英散用药1 d后体细胞数均极显著降低（P<0.01），且公英翘芦散组SCC下降幅度明显超过公英散组。公英翘芦散组在给药2 d后体细胞数显著低于公英散组（P<0.05），给药3 d之后均极显著低于公英散组（P<0.01）。用药7 d后，公英翘芦散组治愈率可达68.29%，而公英散组治愈率只达到20.00%，差异极显著（P<0.01）；公英翘芦散组有效率可达97.56%，而公英散组有效率为67.50%，差异极显著（P<0.01），说明公英翘芦散的治疗效果极显著优于公英散（P<0.01）。以上结果说明，公英翘芦散可以作为治疗亚临床奶牛乳房炎的有效药物，为奶牛乳房炎药物的开发提供参考。     

硒的生物学特性与奶牛营养需要的研究进展

对硒的生物学特性,硒对奶牛健康的影响以及奶牛对硒的需要量作了比较全面的综述,为奶牛饲养过程提供科学的参考依据。     

Seroprevalence of chlamydial infection in dairy cattle in Guangzhou, southern China

Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region.     

Soil Chemical Properties and Nutrient Composition of Cocoyam Grown in an Organically Fertilized Soil

ABSTRACT

Field experiments were conducted for two consecutive years to evaluate the influence of cow dung and rice husk application rates on soil chemical properties and nutrient composition of cocoyam cormels. The treatments comprised four rates each of cow dung and rice husk (0, 10, 20, and 30 t/ha) arranged as a factorial experiment using randomized complete block design with three replicates. The treatments were incorporated into the soil 2 weeks before planting of cocoyam each year. After 2 years of cropping, soil samples were collected from the respective plots and analyzed so also cocoyam cormels. Cow dung application positively and significantly (P < .05) affected soil pH, organic matter (OM), and the soil nutrients (r = 0.95, 0.98, and 0.94–1.00, respectively) while rice husk application significantly and positively influenced soil OM, nitrogen and phosphorus (r = 0.98, 0.95, and 0.98, respectively). Aside potassium content that was enhanced, cow dung application did not significantly affect the nutrient composition of the cocoyam cormels. However, 30 t/ha of applied rice husk caused significant reduction in crude protein and fiber contents but significant increase in carbohydrate. There was negative and significant correlation between rice husk and the cormels crude protein (r = ?0.97). A total of 20 t/ha each of the organic fertilizers was found to be optimum for improving soil fertility and invariably yield without compromising the nutrient content of the cocoyam cormels.