VH1-44 gene usage defines a subset of canine B-cell lymphomas associated with better patient survival

The use of specific immunoglobulin heavy chain variable region (VH) genes has been associated with increased patient survival in human B-cell lymphomas (hBCL). Given the similarity of human and canine BCL (cBCL) in morphology and clinical treatment, we examined the choice of VH in cBCL and determined whether VH gene selection was a distinct feature associated with survival time in dogs. VH gene selection and mutational status in 52 cBCL, including 29 diffuse large B-cell lymphomas (cDLBCL, the most common subtype of cBCL), were analyzed by comparison with the 80 published canine germline VH gene sequences. We further examined the prognostic impact of the subgroups defined by these features on canine survival. We found that VH1-44 was preferentially expressed in the majority of the 52 cBCLs (60%) as well as in the majority of the cDLBCL subset (59%). VH1-44 gene expression was associated with a statistically better overall survival (p = 0.039) in cBCL patients, as well as in the cDLBCL subset of patients (p = 0.038). These findings suggest that VH gene selection in cBCL is not random and may therefore have functional implications for cBCL lymphomagenesis, in addition to being a useful prognostic biomarker.     

陕西省羚牛mtDNA控制区序列结构和种群遗传多样性分析

为给羚牛(Budorcas taxicolor)秦岭亚种的保护和管理提供有用的信息,调查陕西省秦岭羚牛3个野生种群线粒体DNA(mtDNA)控制区357bp片段的遗传多样性和种群结构。结果显示,67个羚牛个体碱基组成为A+T的平均含量(56.3%)高于G+C含量(43.7%),3个群体mtDNA控制区的变异位点为60个(约占总位点数的17.29%)。核苷酸多样性(Pi)为0.007 48,平均核苷酸差异数(K)为2.052。67个个体分属4个单倍型,单倍型间的平均遗传距离(P)为0.127。说明秦岭羚牛群体mtDNA控制区序列遗传多样性较低,群体间的基因交流较少,有可能出现近亲繁殖的情况。     

凉山不同环境区域烟叶质量分析

为探明凉山不同环境区域烟叶的质量特征,对沿江地带干热河谷地区、山地区域、盆地坝区的烟叶综合质量进行了比较和评价。结果表明,烟叶外观质量表现为山地区域＞河谷区域＞盆地坝区;盆地坝区烟叶烟碱、总氮含量最高,总糖、还原糖、氧化钾、氯含量及总糖／烟碱、钾／氯指标最低,河谷区域与山地区域烟叶化学成分指标均差别不大,但山地区域烟叶总糖、还原糖、氧化钾含量和总糖／烟碱、钾／氯均略低于河谷区域烟叶;山地区域烟叶物理特性最好,盆地坝区烟叶最差;烟叶各类香气物质含量和评吸质量均表现为山地区域＞河谷区域＞盆地坝区。山地区域烤后烟叶综合质量最高,河谷区域烟叶综合质量好于盆地坝区。     

阿尔金山北麓野骆驼生境植被调查研究

阿尔金山北麓是野生双峰驼的主要分布地区之一,在该地区野生双峰驼的生境内设立样线样方,对野生双峰驼生境内沙漠植被组成、地理区系和群落多样性等特征进行调查分析。结果表明,该地区的沙漠植物较为稀少,有9科17属17种植物,植物区系倾向于集中在少数大科和单种属内,优势科属的组成较少;该地区植物科的地理分布型有3种,属有5种地理分布型和2个变型,总体植物区系的温带性质较为明显,并呈现了与古地中海成分的相关性;该地区的植物群落物种组成稀少,结构单一,群落多样性指数较低,这与荒漠植物生境的严酷性有密切关系。     

东海区海洋捕捞产量的时间序列分析研究

利用东海区1956－1995年计40年的海洋捕捞产量统计数据，研究东海区小黄鱼等10个主要种类渔业的发展过程。结果表明：东海区主要种类渔业发展过程符合渔业发展的一般规律，即经历未开放、开发中、成熟和衰退4个阶段，且主要种类如带鱼、大黄鱼、马面Tun等渔业大多处于成熟期或衰退期，从而预测其发展趋势，并提出必须加强渔业管理、控制捕捞强度等使渔业实现可持续发展的管理措施。     

1961~2013年天峻地区草地气候生产力的变化分析

依据天峻县1961~2013年的年平均气温和降水量气象观测资料,采用目前常用的Thomthwaite Memorial模型计算了草地气候生产力TSPV。结果表明,天峻地区的TSPV总体呈现显著增加趋势,尤其是2000年以来,达到年代最大;平均气温对天峻地区TSPV的影响占主导地位,而年降水量的影响相对较小,但不容忽视;气候变化对TSPV影响明显,"暖湿型"气候对草地植被生长最有利,平均增产9.2%~18.3%,"冷湿型"、"冷干型"、"暖干型"气候对草地植被生长均较不利。     

瓠瓜高山栽培品种比较试验

比较分析了2种类型的6个瓠瓜品种在杭州高山露地栽培条件下的生育期、商品性和产量等性状指标。试验结果表明,长棒形品种以浙蒲6号和越蒲2号综合性状优良,在杭州高山地区具有极大推广前景。中棒形品种以浙蒲8号商品性较好,但产量相对偏低,可通过进一步研究其配套栽培技术来提高产量促进推广应用;越蒲1号目前不适合杭州市场消费习惯,但产量高,可作为外销储备品种。     

西藏昌都地区实施草场承包经营责任制的现状和对策

介绍了西藏昌都地区生态环境的基本状况和该地区草场承包经营责任制的发展过程,进一步认识到落实和完善草场承包经营责任制对该地区的重大意义,及其对昌都地区畜牧业发展的重要作用。科学分析了该地区发展草场承包经营责任制的必要性,以及实施草场承包经营责任制进程中存在的问题和所面临的困难,并提出了有利于进一步促进和完善该地区实施草场承包经营责任制的参考性建议。     

Genetic divergence of kingfish from Japan, Australia and New Zealand inferred by microsatellite DNA and mitochondrial DNA control region markers

ABSTRACT: Genetic polymorphism in kingfish, collected from coastal waters of Japan, Australia and New Zealand, were examined using microsatellite (MS) DNA and mitochondrial DNA (mtDNA) control region markers. Sixteen to 25.7 alleles per locus were observed in three MS markers, while the average observed (and expected) heterozygosities were 0.782 (0.918), 0.750 (0.809) and 0.650 (0.888) for Australian, Japanese and New Zealand kingfish, respectively. Twelve mtDNA haplotypes were detected by the digestion of control region sequences with five endonucleases: Hae III, Hinf I Mbo I, Rsa I and Taq I. Significant genetic divergence was observed between the kingfish population from Japan and those from Australia–New Zealand. There was no significant differentiation among the Australian and New Zealand population samples.