基于线粒体DNA控制区（mtDNA Dloop）序列分析瓢鸡的遗传多样性

 采用PCR产物直接测序技术对云南省镇沅县50只瓢鸡样品的线粒体DNA控制区（mtDNA D loop）第Ⅰ高变区序列进行了分析。结果表明：在所分析的Dloop区序列中（520bp）,T,C,A,G平均含量分别为30.4%,29.6%,27.4%,12.6%;共检测到27个核苷酸多态位点,其中24个为转换,3个为颠换,序列突变率为5.19 %;序列存在13种单倍型,单倍型多样度(H)为0.883±0.028,核苷酸多样度(π值) 为0.01503±0.00099,表明瓢鸡群体内遗传变异较丰富。瓢鸡样品可划分为A,B,E,F,G等5个世系,在NJ无根系统树上聚为5大枝,揭示瓢鸡在遗传组成上具有5个母系血统来源。     

三维激光扫描技术在植物建模中的应用

三维激光扫描技术已经被广泛应用到各个领域,然而针对植物对象的三维扫描以及数据处理,尚存在诸多问题。从植物三维建模的角度出发,分别应用三款不同类型的激光扫描仪对植物形态结构进行扫描实验,分别以代表性的植物对象叶片为实验对象,探索三维激光扫描技术在植物数据获取与模型三维重建中的应用,并且针对扫描实验及数据处理过程中出现的问题进行分析,提出相应的解决方案。     

几种药剂防治竹瘿蜂试验

竹瘿蜂（Aiolomorphus rhopalodes Walker)是早竹主要害虫之一，1a发生1代，经打孔注药防治试验，结果表明，在6月上旬，用40％氧化乐果、50％甲胺磷乳油防治，药效最显著，效果达94％以上。     

Enzootic bovine leukosis application of E.L.I.S.A. technique for the detection of antibodies

A simple microplate method of enzyme-linked immunosorbent assay (E.L.I.S.A.) for detecting antibodies to bovine leukosis virus (B.L.V.) is described. The antigen consisted of a solution containing the two major antigens of the B.L.V. (gp 51 and p 24) obtained by a technique of purification using CN-Br activated Sepharose 4B. This E.L.I.S.A. was compared with the agar gel immunodiffusion test (A.G.I.D.T.) in a study of 545 bovine sera. The total discrepancy rate between the two tests was 11% with a better sensitivity for E.L.I.S.A.     

Priority setting in research for sustainable land use: the case of the Adja Plateau,Benin

TheProjet Recherche Appliquée en Milieu Réel is situated on the Adja Plateau, Southwest Benin. The project is responsible for a FSR&D programme that aims at sustainable use of the so-calledTerres de barre. Technologies tested in this context include seasonal fallowing, usingMucuna pruriens cv. group Utilis (weed control, green manure), planted fallows using fast-growing species such asAcacia auriculiformis and hedgerow intercropping systems (various woody auxiliary species). In this paper the local farming system is analyzed to identify key parameters that determine the possibilities for technology adoption. Parameters include: function and history of fields; tenureship; the field's position with regard to fertility flows in the farming system.The paper also aims at understanding the reasons behind low crop yields, in this case maize. An analysis of the soil's chemical fertility, using a computer-based fertility assessment model and data from literature, shows some anomalies with respect to the availability of nutrients from organic matter. Data presented on the use of green manures and oil palm fallow compared to cultivated fields make it likely that organic matter management is a crucial factor in sustainable use of these soils (maintenance of soil structure and soil life). Given key parameters, a matrix is used to determine potential niches in the farming system for the various biomass production technologies mentioned previously, including the locally developed planted fallow based on oil palm.     

马尾松和杉木干基不同高度直径与胸径相关关系的研究

研究了马尾松的杉木离地面２０ｃｍ以下干基５个不同高度直径与胸径的相关关系，建立起精度达９８．６％，马尾松胸长与干基１０ｃｍ高处直径的数学模型和精度达９８．８％的杉木胸径与干基１５ｃｍ高处直径的数学模型。此两模型可用于被盗杉木的材积核实，森林资源采伐审计和马尾松割脂林木的胸径修正。     

伪狂犬病病毒Ea株糖蛋白gD基因的表达及基因免疫

构建了2个利用人类巨细胞病毒（HCMV）的启动子启动表达伪狂犬病病毒Ea株糖蛋白gD基因的真核表达质粒pCIDI和pcDDI，体外转染BHK－21细胞，用间接免疫荧光法检测，证实糖蛋白gD在细胞中得到表达。用表达质粒pCIDI和pcDDI作为核酸疫苗免疫BALB/c小鼠，ELISA检测小鼠血清中抗伪狂犬病病毒的抗体，结果其滴度为1：128－1：1512。初步证实，用gD基因作为核酸疫苗免疫动物，可以激活机体的免疫应答反应。     

砂藓抗旱相关基因磷脂酶D的克隆和表达分析

磷脂酶D是广泛存在于植物中的一种磷脂酶,在细胞结构组成和生理功能的变化中具有非常重要的作用.在本研究中,以在砂藓(Racomitrium canescens)转录组数据库中获得的一条与抗旱相关的磷脂酶D基因为基础,采用RT-PCR技术克隆得到该基因的全长CDS序列,命名为RcPLD.生物信息学分析结果表明,RcPLD基...     

基于Kinect相机的油麦菜自动化三维点云重建

为了解决传统三维点云重建过程中人工调参费时、费力,且精度得不到保障等问题,提出了一种三维点云自动化配准算法,并应用于油麦菜三维重建.使用Kinect相机采集油麦菜不同视角下的点云数据,通过配准实验分析配准参数的变化规律,继而建立了配准评价体系,实现了两片点云的自动化配准,并通过最小化匹配误差积累将多幅点云变换到同一基准...