Inhibitory effect of 1,25-(OH)2D3 on proliferation of airway smooth muscle cells from passively-sensitized patients

AIM: To investigate the effects of 1,25-dihydroxyvitamin D₃ on the proliferation of passively-sensitized human airway smooth muscle cells (HASMCs), and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)₂D₃ was used as the interventor.The effect of 1,25-(OH)₂D₃ on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by flow cytometry.The expression of proliferating cell nuclear antigen (PCNA) was measured by the method of immunocytochemical staining.RESULTS: 1,25-(OH)₂D₃ at the concentrations of 10^-9-10^-7 mol/L markedly inhibited the cell proliferation and the maximum effect was observed at the concentration of 10^-7 mol/L.This concentration of 1,25-(OH)₂D₃ markedly suppressed the PCNA-positive rate and hampered the G₁/S transition in HASMCs passively-sensitized by asthmatic serum.CONCLUSION: 1,25-(OH)₂D₃ has direct inhibitory effects on the proliferation of passively-sensitized HASMCs in vitro, which may be concerned with the beneficial role of 1,25-(OH)₂D₃ on the prevention and therapy of asthmatic airway remodeling.     

Virtual Studio System Technology

Virtual studio system (VSS), a new technology based on virtual reality, develops fast in recent years. Virtual studio concept replaces real background sets with computer generated 3D synthetic and combines real foreground action, with computer generated backgrounds. As VSS need not construct real background sets and has little limitness of time and space, it is regarded as a revolution in TV programs. This paper mainly discusses about the background, technology feature, application and future.     

玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究

玉米幼叶愈伤组织的形成能力因其形态学部位的不同而呈显著差异, 从基部向上依次递减, 幼叶外植体的内源IAA和ABA水平亦相应地呈递减趋势。 2, 4-D及与BA组合对基部切段的愈伤组织形成无显著影响, 愈伤组织生长符合Logistic曲线, 单独使用BA不利于愈伤组织形成; 采用2 mg/L 2, 4-D与0.5 mg/L BA的组合最有利于胚性愈伤     

小麦(T. aestivum)Waxy-D1 基因缺失材料的发现及分析

利用SDS-PAGE技术, 从河北省及其它省引进小麦地方品种900份中, 鉴定出了普通小麦Waxy-D1基因缺失材料一份, Waxy-B1基因缺失材料6份。 这些材料的发现, 为我国开展品质育种研究打下了基础, 尤其是Waxy-D1隐性材料尤为稀少, 是至今为止世界上发现的第二份含有该隐性基因的地方品种。 在小麦淀粉遗传控制研究和品质育种     

OpenGL在粮仓温度场可视化中的应用

根据粮仓温度监测系统采集到的温度数据,首次在VC 6．0MFC平台上,运用3DSMAX,OpenGL图形工具包,实现了整个粮库外景三维建模以及粮仓温度场的动态三维重现。从而可直观地了解粮仓内部的温度情况。     

山竹果皮总黄酮富集工艺研究

研究D101大孔树脂富集山竹果皮总黄酮的工艺。通过静态、动态相结合的方法,确定D101大孔树脂富集的最佳工艺参数。结果表明,D101大孔树脂对总黄酮动态解析附的效果好于静态,动态解析附的最佳条件为:以流速2 m L/min上样30 m L 2 mg/m L的提取物,且用60 m L 70%乙醇以2 m L/min的流速洗脱,此条件下提取物总黄酮含量由原先的57.8 mg/m L增加到174.2 mg/m L。     

Study on high and steep slope stability Based on 3D laser scanning

Dangerous mountain construction projects block of high and steep slope stability is the important issue of security and disaster prevention, investigation on the surface of the dangerous mountain high and steep slope rock mass structure is the key to the analysis of the problem. The high slope of a major project of the Lancang River, the application of 3D laser scanning technique to study the long-distance survey analysis of rock mass structure analysis method to analyze the distribution of the surface space of the high and steep slope rock mass structure, statistical analysis direction of the advantages of the structure surface and the distribution of rock blocks, as the basis for the application of limit equilibrium analysis method to study the stability of rock blocks of the high and steep slope.     

超高产杂交稻剑叶的光抑制及其77K荧光光谱特性

通过对培矮64S/E32和汕优63进行高光胁迫结果表明: 与籼型杂交稻汕优63比较, 培矮64S/E32的Fv/Fm和ΦPSⅡ、PSⅡ和PSⅠ荧光强度下降的幅度以及F685/F742和F694/F742比率的变化较小。用D1蛋白合成抑制剂CAP处理时,培矮64S/E32 的Fv/Fm和qP下降程度稍高于汕优63,而用叶黄素循环抑制剂DTT处理,结果则反之。表明超高产水稻品     

玉米D亚族bZIP转录因子基因的数据库挖掘、分析、克隆与表达

碱性亮氨酸拉链(basic leucine zipper, bZIP)是真核生物特有的转录因子家族,在高等植物基因表达与调控中起重要作用。本文通过对玉米基因组数据库中收录的全长cDNA序列全基因组范围的bZIP分析,发现其中25个序列编码D亚族bZIP转录因子。针对这些序列进行生物信息学分析、染色体分布和直系同源组的分类分析,发现部分序列可能是由同一基因座编码,但不同方式剪切形成的。对部分基因克隆和测序发现了更多的剪切形式。定量检测其中3个基因在ABA和干旱胁迫条件下的表达发现,其中1个受ABA诱导,2个受ABA抑制,但均不受干旱胁迫影响。表明玉米中D亚族基因可能参与ABA响应。