Evaluation of maturity by use of pH indicators in sawdust-based cultures ofLentinula edodes

Colorimetric pH indicators were examined for their use in judging the culture maturity of sawdust-based cultures of shiitake,Lentinula edodes (Berk.) Pegler. Bromophenol blue (BPB) was the preferred indicator for judging culture maturity. Culture pH changes were detected as changes in color from bluish purple to yellow by direct spraying. The pH values of such cultures varied from 6.3 before inoculation to 4.0 during the fruiting stage. Culture maturity assessment can be used to control fruit body flush timing. Measurement of BPB staining inL. edodes sawdustbased cultures showed a good correlation with fruit body yield of the first flush. This method was concluded to be of value for detecting fruiting potential and therefore may be useful for evaluating flush timing forL. edodes cultivators using sawdust-based methods.     

弘扬茶文化促进社会主义和谐社会建设

中国是茶的故乡,是世界上茶历史最悠久、茶资源最丰富、茶文化最深厚的国家.茶在中国始终扮演着社会交往的最佳礼物和媒介,是人与人之间友谊的桥梁.茶文化的本质是"和谐文化",它引导人们与自然和谐相处,启示人们在人与人的关系上,要彼此尊重,加强交流,启发人们感悟身心的统一.大力弘扬茶文化精神,是建设和谐文化的一笔重要资源,是构建和谐社会的重要载体.     

鸡死胚中绿脓杆菌的分离与鉴定

对来自西宁市某养鸡场的5只死胚,进行分离培养得到5株细菌,经染色镜检、培养特性观察、动物实验,以及生化鉴定等试验,初步诊断这5株分离菌株均为绿脓杆菌。     

Effects of permanent magnetic fields on the proliferation of Phalaenopsis protocorm-like bodies using liquid medium

Magnetic fields (MFs) have been applied for the first time in orchid micropropagation. Protocorm-like bodies (PLBs) – approximately 3 mm in diameter – first derived from leaf segment culture of Phalaenopsis Gallant Beau ‘George Vazquez’, were subcultured every 2 months, and served as initial explants. The proliferation of Phalaenopsis PLBs in liquid medium in the Miracle Pack^® culture system was affected by the action of different intensities and polarities of MFs: 0.1, 0.15 and 0.2 Tesla (T) at North (N) and South (S) poles. The MF of 0.1 T – S resulted in the greatest fresh weight of regenerated PLBs. The average number of neo-PLBs formed per clusters in the PLB treated by MF: 0.1–0.2 T was decreased compared to the control exposed to natural MF (5 × 10⁻⁶ T). The proliferation of PLBs under 0.15 T – MF at both N and S poles for 2 and 7 weeks demonstrated that a longer duration of exposure to an MF of 0.15 T, regardless of the polarity, resulted in greater biomass of newly formed PLBs and smaller average number of newly formed PLBs. The S pole of MF had stronger effects on Phalaenopsis PLBs proliferation than the N pole did in all treatments.     

不同水肥对猩红栎轻基质容器苗生长的影响

对1年生猩红栎轻基质容器苗进行不同的水肥处理试验,结果表明,A1B2组合(浇水1天1次,施肥0.2 g/株)处理时,猩红栎轻基质容器苗质量最好;水分为猩红栎容器苗生长的重要因子。     

邓小平农民思想与新型农民培养

邓小平农民思想是邓小平理论中的重要组成部分,是运用马克思主义基本原理的理论成果。新型农民培养问题是当今社会理论界研究的热点问题,基于邓小平农民思想理论基础,为解决新型农民培养问题找到新思路和新方法,也会对我国三农问题的解决和和谐社会的构建产生重要意义。     

蝙蝠蛾拟青霉高产腺苷液体培养基优化

为了提高蝙蝠蛾拟青霉菌株RCEF0936腺苷的代谢量,以腺苷含量为指标,采用高效液相色谱法(HPLC),在单因子试验的基础上通过正交试验对其高产腺苷的液体培养基进行优化研究。结果表明,在培养温度25℃、摇床转速140rpm/min、摇瓶装液量100ml/250ml、接种量5%、培养时间6d的条件下,蝙蝠蛾拟青霉菌株RCEF0936的高产腺苷的最佳液体培养基配方为:葡萄糖2%,酵母浸出粉2%,硫酸锰0.05%,硫酸锌0.05%;试验中选择的4种因子对蝙蝠蛾拟青霉菌株RCEF0936菌丝体中腺苷含量的影响由大到小依次是硫酸锌酵母浸出粉硫酸锰葡萄糖,且均达到极显著水平(P0.01)。利用优化的培养基培养的蝙蝠蛾拟青霉菌株RCEF0936菌丝体中腺苷含量可达到2.85mg/g,比优化前提高了390%,经统计学分析,两者差异极显著(P0.01)。     

不同培养液中卵形隐藻的生长和主要生化组成的比较

于１９９３年５月￣７月研究了５种培养液中形隐藻的生长及其主要生化成分的组成。结果表明，作者设计的ＦＰＷ培养液培养的卵形隐藻的最大密度和特定生长率均明显高于目前室内研究中普遍使用的ＷＣ培养液培养的相应值；氨基酸及必需氨基酸的比例非常接近；但蛋白质水平及多不饱和脂肪酸比例稍低。ＦＰＷ组成的简单性及由池塘水配制的特点表明，在隐藻规模化培养时，ＦＰＷ较ＷＣ具有更大的实用性。     

甜菜原生质体培养再生植株的研究

以栽培甜菜的未授精胚珠或胚为外植体，在ＭＳ附加ＮＡＡ和ＢＡ的培养基上诱导愈伤组织，选择胚性愈伤组织进行继代培养，从该愈伤组织分离原生质体并以液体浅层法或半固体琼脂糖法培养在一系列培养基上，植板率为０．０１％～１．２％．再生愈伤组织形成后，转入固体培养基（ＭＳＢ）上培养，再转入分化培养基上分化出苗，分化率可达２．５％，由愈伤组织上切下分化苗在生根培养基上很容易诱导生根。     

工厂化循环水鱼类养殖脱氮趋零排放系统研制

为摆脱生物膜脱氮技术的制约,提高循环水脱氮处理效率,营造适合鱼类养殖的富氧环境,增加鱼类养殖经济效益;基于鱼菜共生生态及经济效益,提供一种设计合理、高效环保的工厂化循环水鱼类养殖脱氮趋零排放系统,重点解决种养殖水体营养浓度需求不一致的关键技术瓶颈。将养殖污水经过电迁移和膜浓缩工艺结构,得到能够满足植物营养需求的高氮水,同时得到能够用于鱼类养殖池的循环水,解决鱼菜营养物质浓度需求矛盾,大大提高植物和鱼类的产量,获取鱼菜共生效益。同时,为方便对工厂化循环水鱼类养殖脱氮趋零排放系统的操作,设置了总控制平台和分控制平台,具备24h集中与分布控制性能。应用试验分析表明,经系统处理的养殖水体可以满足鱼菜生长需求,系统研制是成功的。