饲养水平和遗传特性对XX;mas-1/mas-+鲤鱼(Cyprinus carpio L.)性别决定的影响

饲养水平对E4×E5 (XX ;mas- +/mas- +×XX ;mas- 1/mas - 1)鲤鱼 (CyprinuscarpioL )F1代的性别决定有显著影响 ,高饲养水平和低饲养水平处理组中雌性比率分别为 5 1 5 %和 4 1%。遗传特性对E4 5×E5 (XX ;mas- 1/mas - +×XX ;mas- 1/mas- 1)F1代的影响比饲养水平的影响显著 ,在 2个处理组中只发现 1～ 2尾雌性。 2个饲养水平对E4×E4 5 (XX ;mas- +/mas- +×XX ;mas- 1/mas - +)F1代的影响差异显著 ,高饲养水平 2个处理组中雌性比率分别为 86 2 %和 76 8% ,而低饲养水平 2个处理组中雌性比率分别为 76 8%和 5 7 9% ,高饲养水平处理组的雌性比率与低饲养水平处理 1组 (雌性比率 5 7 9% )相比差异显著。 2个饲养水平条件下E4×E5和E4×E4 5后代的XX ;mas - 1/mas - +鲤鱼性别转化率之间没有显著差异。     

海娜中黄酮类化合物的提取及含量测定

用正交试验提取海娜花和叶中的黄酮类化合物,并在510 nm处测定其吸光度值,计算其含量.结果表明,海娜花和叶中黄酮类化合物的提取分别是花以65℃时、用浓度为95%的乙醇提取1.5 h,叶以65℃时,用浓度为95%的乙醇提取2.5 h最好,得出的海娜花和叶中黄酮含量可分别高迭2.356%和1.569%.     

浅谈各地市花及市树的选择

市树市花的选择过程是规范和具体的,其本身能够代表城市的文化内涵、人文精神和地域特征。除此以外。市树市花的确定还能够有效的推进城市的文化建设和城市基础设施建设,增强人们的城市归属感和自豪感。     

微波消解−电感耦合等离子体质谱快速测定葡萄籽提取物微量元素的研究

采用高压微波消解,建立葡萄籽提取物微量元素ICP−MS检测方法,并对葡萄籽中47种微量元素进行测定,应用芹菜成分分析标准物质对该方法进行验证。结果表明：该方法线性关系良好,相关系数为0.9959～0.9999,微量元素的检出限小于0.1 μg/L。测定值均在标准值范围内,连续测定6次相对标准偏差（RSD）为0.1%～5.4%,回收率为82.7%～112.8%。11批葡萄籽提取物中,K、Ca、Mg、Na、Fe元素含量高,Al、Mn、Ni、Cu、B、Zn、Rb、Sr、Sn、Ba、Mo元素相对较高,Pb、As、Hg、Cd、Cr及15种稀土元素含量较低。验证结果表明,该方法专属性强、灵敏度高。     

葡萄新品种--爱格丽

以 3个欧亚葡萄栽培品种及其 2个中间杂交种为亲本 ,经过 15年培育出酿酒葡萄新品种‘爱格丽’ (Ecolly)。该品种除保持亲本的优质、丰产、抗寒性状以外 ,其最突出的特点是抗病性强 ,霜霉病病情指数为 16.5 ,是对照的 46.6%。适合酿造优质干白葡萄酒。     

三个地方鸡种产蛋性状配合力测定

以文昌鸡、如皋黄鸡、仙居鸡为素材,采用完全双列杂交测定配合力,统计各周龄体重、开产蛋重、产蛋高峰期蛋重、开产至40周龄料蛋比、开产至40周龄平均产蛋率,计算一般配合力和特殊配合力效应值.结果表明:父本对开产至40周龄平均产蛋率及入舍母鸡产蛋数贡献较大,母本对蛋重影响较大;文昌鸡♂×仙居鸡♀组合开产至40周龄料蛋比最低、40周龄产蛋数最高,为最佳配套组合,可在生产中配套使用.     

液相色谱—串联质谱法测定动物源性食品中4种β_2~-受体激动剂类药物残留

建立了动物源性食品中4种β2-受体激动剂克伦特罗、莱克多巴胺、沙丁胺醇和特布他林的高效液相色谱—串联质谱测定方法。样品经5%的三氯乙酸酸解提取,Waters Oasis MCX固相萃取柱净化,然后用Aglient ZORBAXSB-Aq(3.5μm,3.0 mm×100 mm)色谱柱分离,以0.1%甲酸乙腈溶液和0.1%甲酸水溶液为流动相进行梯度洗脱,基质匹配标准溶液内标法定量。结果表明,4种β2-受体激动剂在质量浓度1.0~100 g/L内呈良好的线性关系,相关系数R均大于0.995 0;检出限(S/N≥3)均为0.1μg/kg,定量下限(S/N≥10)为0.25 g/kg,在0.5,2,5μg/kg3个质量分数添加水平,回收率在81%~108%,相对标准偏差在1.3%~9.4%。方法灵敏度高、定性准确可用于各种动物源性食品中4种β2-受体激动剂残留的快速检测。     

一种准确简便的东方白鹤性别分子鉴定方法

东方白鹤为国家Ⅰ级重点保护动物，在自然条件下繁殖率很低，进行迁地保护及再引入措施对遗传多样性保护具有举足轻重的作用，成功地进行性别鉴定是其笼养人工繁殖的前提。本研究对4只东方白鹤，运用CHD基因的一对引物2550F／27181K进行了准确简便的性别鉴定，并对其序列结合其他5种远缘鸟类相应序列进行分析，证实了此种方法的科学性和可信度，分析了性别鉴定机理。这种安全、方便、准确的性别分子鉴定方法将有助于东方白鹤迁地保护及再引入措施的实施。通过分析可以看出，此种方法未来存在着广阔的应用前景。     

Comparison of different pH measurement methods in meat

The accuracy of portable pH meters and the nitrazine yellow method was compared with the reference method by determining the pH of 74 beef and 96 pork muscles. The pH was measured directly from the muscle. The results showed statistically significant differences (p less than 0.001) between the different electrometric combinations. Combinations of portable pH meters with puncture electrodes gave systematically higher pH values than the reference method. These differences were not very large but they may be of practical significance. The use of a piercing cover on the electrode to help the insertion of the electrode into the meat is not recommended, since it may cause a rise in pH values. Electrometric methods were found to be more precise than the nitrazine yellow method. On the basis of these findings there still is a need of further harmonization of the methods used for pH measurement of meat.     

Onset of luteal activity in non-foaling mares during the early breeding season in Finland.

The luteal activity in mares was studied in the Equine Research Station (ERS) and in trotting stables (TS) in South-Finland. The mares were Standardbreeds in the TS and mainly Finnhorses in the ERS. Between January and June blood was collected once a week for serum progesterone determinations. The mares in the ERS were distributed in 1 of 3 groups: three-years old not yet in training (N = 38), brood mares (N = 21) and mares in training (N = 47). A 4th group was the mares in training in the trotting stables (N = 73). Every 5th mare in the ERS and every 4th mare in the trotting stables were cycling already at the beginning of the year. Onset of luteal activity in anoestrous mares was most common in the middle of May. Over 95% of the mares were cycling at the beginning of June. In the ERS 40% of the Finnhorse mares in training were cycling through the winter. The three-years old and the brood mares were all anoestrous during winter. They started to cycle on average before the middle of May. Anoestrous training mares started before the middle of April. Anoestrous Finnhorse mares began to cycle later than warm blooded mares in all of the groups studied. Mares which had foaled the previous year were more often anoestrous during the winter than dry mares. The time of year when cycling began in a particular mare tended to be the same from year to year (p less than 0.01).